本文選題：不同產(chǎn)地 + 砂仁　； 參考：《中國實驗方劑學(xué)雜志》2017年08期

【摘要】：目的:建立高效液相色譜法(HPLC)同時測定砂仁中原兒茶酸和香草酸的含量,并對不同產(chǎn)地砂仁的原兒茶酸和香草酸含量進行比較。方法:采用Phenomenex Luna C_(18)色譜柱(4.6 mm×250 mm,5μm),以甲醇-0.1%甲酸水為統(tǒng)計相進行梯度洗脫,柱溫35℃,流速0.8 mL·min~(-1),進樣體積15μL,檢測波長260 nm。結(jié)果:原兒茶酸在0.000 7~0.008 9 g·L~(-1)呈良好的線性關(guān)系,平均回收率100.4%(RSD 3.2%);香草酸在0.000 6~0.030 3 g·L~(-1)呈良好的線性關(guān)系,平均回收率99.3%(RSD 2.8%)。對24批不同產(chǎn)地砂仁樣品的原兒茶酸及香草酸含量測定結(jié)果進行聚類分析,結(jié)果顯示不同產(chǎn)地砂仁樣品分為兩大類,一類為陽春砂與綠殼砂,另一類為進口殼砂仁、艷山姜偽品及加工方式為生曬的云南產(chǎn)陽春砂。結(jié)論:該方法簡便、準(zhǔn)確、重復(fù)性好,可用于不同產(chǎn)地砂仁中原兒茶酸和香草酸含量測定,為鑒別不同產(chǎn)地的砂仁樣品提供參考。砂仁中香草酸含量受其加工方式影響含量變化明顯。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for simultaneous determination of protocatechuic acid and vanillic acid in Amomum villosa, and to compare the contents of protocatechuic acid and vanillic acid in Amomum villosum from different areas. Methods: a Phenomenex Luna Che _ (18) column of 4.6 mm 脳 250 mm ~ (5 渭 m) was used for gradient elution with methanol-0.1% formic acid water as the statistical phase. The column temperature was 35 鈩，

本文編號：1863343