本文選題：纖維素紙板 + 碳化電極��； 參考：《環(huán)境科學(xué)學(xué)報》2017年02期

【摘要】：采用Solexa高通量測序技術(shù)和蛋白定量方法,分析了碳化纖維素紙板制備的層狀波紋碳(LCC)電極與石墨板(GP)電極在生物電化學(xué)系統(tǒng)運(yùn)行過程形成的生物膜微生物群落結(jié)構(gòu)和生物量差異.結(jié)果表明,LCC電極良好的生物電化學(xué)性能不僅取決于其良好的導(dǎo)電性能,還與其表面生物膜微生物群落結(jié)構(gòu)有關(guān);LCC電極表面生物膜微生物量高,且對產(chǎn)電微生物的富集效果好.LCC電極與GP電極表面生物膜分別得到16S rRNA基因V3區(qū)優(yōu)化序列12643條和12837條,經(jīng)97%相似度歸并后獲得的OTUs數(shù)量分別為2786和3130;α多樣性分析顯示,GP電極生物膜微生物多樣性相對更豐富.Proteobacteria、Firmicutes和Bacteroidetes在兩種電極生物膜中含量最為豐富,這3個門細(xì)菌序列數(shù)分別占總序列數(shù)的76%(LCC)和85%(GP).在屬分類水平上,LCC電極生物膜由383個屬的細(xì)菌構(gòu)成,而GP電極生物膜則有456個屬.深入分析電極生物膜微生物群落結(jié)構(gòu)有助于進(jìn)一步認(rèn)識LCC電極提高生物電化學(xué)系統(tǒng)產(chǎn)電效率的機(jī)理.
[Abstract]:Solexa high-throughput sequencing technique and protein quantification method were used. The biofilm microbial community structure and biomass difference between laminated corrugated carbon dioxide (LCC) electrode and graphite plate GPCC electrode prepared by carbonized cellulose paperboard in the operation of bioelectrochemical system were analyzed. The results showed that the good bioelectrochemistry properties of LCC electrode not only depended on its good electrical conductivity, but also related to the structure of biofilm microbial community on the surface of LCC electrode and the high biomass of biofilm microorganism on the surface of LCC electrode. The optimal sequence of 16s rRNA gene V3 region was obtained from the biofilm on the surface of LCC electrode and GP electrode, respectively, and the optimized sequences were 12643 and 12837, respectively. The number of OTUs obtained by merging 97% similarity was 2786 and 3130, respectively, and 偽 diversity analysis showed that the microbial diversity of biofilm was relatively more abundant. Proteobacteriae Firmicutes and Bacteroidetes were the most abundant in the biofilm of the two electrodes. The number of these three bacteria accounted for 76% of the total number of sequences, and 85% of the total number of bacteria. The LCC electrode biofilm is composed of 383 genera of bacteria and 456 genera of GP electrode biofilm. Further analysis of the microbial community structure of the electrode biofilm is helpful to further understand the mechanism of the LCC electrode increasing the efficiency of the bioelectrochemical system.
【作者單位】： 廈門理工學(xué)院環(huán)境科學(xué)與工程學(xué)院;中國科學(xué)院城市環(huán)境研究所;江西師范大學(xué)化工學(xué)院;
【基金】：福建省中青年教師教育科研項(xiàng)目(No.JA15372) 國家自然科學(xué)基金(No.51208490) 廈門理工學(xué)院高層次人才科技類項(xiàng)目(No.E2015028)~~
【分類號】：O646

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