發(fā)布時(shí)間：2018-05-06 06:31

  本文選題：電化學(xué)生物傳感器 + 比率�。� 參考：《青島大學(xué)》2017年碩士論文

【摘要】：近年來(lái),由于人口老齡化日益嚴(yán)重、生態(tài)環(huán)境遭到破壞、不健康的生活方式等多方面因素的影響,腫瘤患者人數(shù)逐年增加。而腫瘤化療也取得了相當(dāng)大的突破,腫瘤患者的生存時(shí)間被大大延長(zhǎng)。然而患者機(jī)體由于代謝不完全導(dǎo)致的藥物殘留形成的嚴(yán)重副作用也會(huì)因此對(duì)人體造成二次傷害。因此,藥物治療在一定程度上受到了限制。測(cè)定體液中殘余藥品,尤其是血液樣品中藥品的濃度對(duì)于用藥安全的評(píng)估具有重要意義。電化學(xué)傳感器因其具有靈敏度高、選擇性好等優(yōu)點(diǎn),一直是分析科學(xué)中一個(gè)非�；钴S的研究前沿。隨著對(duì)傳感器的結(jié)構(gòu)構(gòu)建過(guò)程的不斷深入探究,比率電化學(xué)生物傳感器越來(lái)越廣泛地引起科研工作者的關(guān)注。本文基于比率電化學(xué)檢測(cè)方法,構(gòu)建電化學(xué)傳感器用于抗腫瘤藥物如鹽酸阿霉素DOX和槲皮素Qu的檢測(cè):一、基于裸玻碳電極GCE檢測(cè)抗腫瘤藥物DOX的比率電化學(xué)生物傳感器。利用DOX與亞甲基藍(lán)MB電化學(xué)活性高的特性,將二者直接加入電解液中,而無(wú)需對(duì)電極進(jìn)行任何修飾;DOX與GCE之間發(fā)生氧化還原,出現(xiàn)顯著的電信號(hào);而MB作為參比出現(xiàn)穩(wěn)定的信號(hào);在控制MB濃度不變的情況下,隨著DOX加入量的增大,DOX引起的電信號(hào)逐漸增強(qiáng);通過(guò)電化學(xué)工作站對(duì)DOX和MB的電信號(hào)進(jìn)行檢測(cè),實(shí)現(xiàn)了對(duì)DOX的比率雙重電信號(hào)快速和高靈敏檢測(cè)。比率法可以明顯降低源自環(huán)境和個(gè)人操作因素的電化學(xué)傳感器的固有系統(tǒng)誤差和偶然誤差,從而顯著增強(qiáng)實(shí)驗(yàn)方案的再現(xiàn)性和穩(wěn)定性。二、基于比率檢測(cè)方法,我們通過(guò)直接使用裸GCE作為信號(hào)傳感平臺(tái)構(gòu)建了一個(gè)簡(jiǎn)易的電化學(xué)傳感器來(lái)靈敏地檢測(cè)電解液中的Qu。此外,硫堇TH被設(shè)為內(nèi)部參考電化學(xué)探針。IQu/ITH與[Qu]之間具有良好的線(xiàn)性關(guān)系,同時(shí)檢測(cè)限低至3.1 nM。在實(shí)際食品樣品的干擾實(shí)驗(yàn)中,該比率檢測(cè)方法對(duì)Qu具有高度的選擇性和靈敏性。與之前報(bào)道的研究相比,本實(shí)驗(yàn)取得了更寬的檢測(cè)范圍(0-15μM),該研究方法的優(yōu)點(diǎn)是Qu的電化學(xué)測(cè)定可以直接在電解液中進(jìn)行,類(lèi)似于在水性介質(zhì)中被廣泛認(rèn)可的熒光傳感方法。該方法操作簡(jiǎn)單,應(yīng)用廣泛,可以擴(kuò)展到電解液中的其他類(lèi)似比率電化學(xué)傳感器的構(gòu)建,這將有助于電化學(xué)傳感器的進(jìn)一步發(fā)展。
[Abstract]:In recent years, the number of cancer patients has increased year by year due to the aging of population, the destruction of ecological environment, unhealthy lifestyle and other factors. Tumor chemotherapy has also made a considerable breakthrough, tumor patients survival time has been greatly extended. However, severe side effects caused by incomplete metabolism can also cause secondary injury. Therefore, drug treatment is limited to some extent. It is important to determine the concentration of residual drugs in body fluid, especially in blood samples. Electrochemical sensors have been a very active research frontier in analytical science because of their high sensitivity and good selectivity. With the research on the structure of the sensor, the ratio of electrochemical biosensor has attracted more and more attention of researchers. Based on the ratio electrochemical detection method, an electrochemical sensor was constructed for the detection of antitumor drugs such as adriamycin hydrochloride (DOX) and quercetin qu. Firstly, a ratio electrochemical biosensor based on bare glassy carbon electrode (GCE) was used to detect the antitumor drug DOX. Based on the high electrochemical activity of DOX and methylene blue MB, they were directly added into the electrolyte without any modification of the electrode between dox and GCE. MB is used as a reference signal. When the concentration of MB is kept constant, the electrical signal caused by DOX and MB is gradually enhanced with the increase of the amount of DOX, and the electrical signals of DOX and MB are detected by electrochemical workstation. The rate dual signal detection of DOX is fast and highly sensitive. The ratio method can obviously reduce the inherent systematic error and accidental error of the electrochemical sensor derived from the environmental and personal operating factors, thus significantly enhancing the reproducibility and stability of the experimental scheme. Secondly, based on the ratio detection method, we constructed a simple electrochemical sensor by using naked GCE as the signal sensing platform to detect the Qui in electrolyte sensitively. In addition, thionine th has a good linear relationship with [qu] as an internal reference electrochemical probe. The detection limit is as low as 3.1 nm. The ratio detection method is highly selective and sensitive to qu in the interference test of real food samples. Compared with previous studies, a wider detection range of 0-15 渭 m ~ (-1) has been obtained. The advantage of this method is that the electrochemical determination of qu can be carried out directly in electrolyte, which is similar to the widely accepted fluorescence sensing method in aqueous media. The method is simple in operation and widely used, and can be extended to the construction of other similar ratio electrochemical sensors in electrolyte, which will be helpful to the further development of electrochemical sensors.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O657.1;TP212.3;TQ460.72

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Yuan-Yuan Yao;Long Zhang;Zi-Fei Wang;Jing-Kun Xu;Yang-Ping Wen;;Electrochemical determination of quercetin by self-assembled platinum nanoparticles/poly(hydroxymethylated-3,4-ethylenedioxylthiophene) nanocomposite modified glassy carbon electrode[J];Chinese Chemical Letters;2014年04期

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本文編號(hào)：1851202