本文選題：電化學生物傳感器 + 比率��； 參考：《青島大學》2017年碩士論文

【摘要】：近年來,由于人口老齡化日益嚴重、生態(tài)環(huán)境遭到破壞、不健康的生活方式等多方面因素的影響,腫瘤患者人數(shù)逐年增加。而腫瘤化療也取得了相當大的突破,腫瘤患者的生存時間被大大延長。然而患者機體由于代謝不完全導致的藥物殘留形成的嚴重副作用也會因此對人體造成二次傷害。因此,藥物治療在一定程度上受到了限制。測定體液中殘余藥品,尤其是血液樣品中藥品的濃度對于用藥安全的評估具有重要意義。電化學傳感器因其具有靈敏度高、選擇性好等優(yōu)點,一直是分析科學中一個非�；钴S的研究前沿。隨著對傳感器的結構構建過程的不斷深入探究,比率電化學生物傳感器越來越廣泛地引起科研工作者的關注。本文基于比率電化學檢測方法,構建電化學傳感器用于抗腫瘤藥物如鹽酸阿霉素DOX和槲皮素Qu的檢測:一、基于裸玻碳電極GCE檢測抗腫瘤藥物DOX的比率電化學生物傳感器。利用DOX與亞甲基藍MB電化學活性高的特性,將二者直接加入電解液中,而無需對電極進行任何修飾;DOX與GCE之間發(fā)生氧化還原,出現(xiàn)顯著的電信號;而MB作為參比出現(xiàn)穩(wěn)定的信號;在控制MB濃度不變的情況下,隨著DOX加入量的增大,DOX引起的電信號逐漸增強;通過電化學工作站對DOX和MB的電信號進行檢測,實現(xiàn)了對DOX的比率雙重電信號快速和高靈敏檢測。比率法可以明顯降低源自環(huán)境和個人操作因素的電化學傳感器的固有系統(tǒng)誤差和偶然誤差,從而顯著增強實驗方案的再現(xiàn)性和穩(wěn)定性。二、基于比率檢測方法,我們通過直接使用裸GCE作為信號傳感平臺構建了一個簡易的電化學傳感器來靈敏地檢測電解液中的Qu。此外,硫堇TH被設為內(nèi)部參考電化學探針。IQu/ITH與[Qu]之間具有良好的線性關系,同時檢測限低至3.1 nM。在實際食品樣品的干擾實驗中,該比率檢測方法對Qu具有高度的選擇性和靈敏性。與之前報道的研究相比,本實驗取得了更寬的檢測范圍(0-15μM),該研究方法的優(yōu)點是Qu的電化學測定可以直接在電解液中進行,類似于在水性介質(zhì)中被廣泛認可的熒光傳感方法。該方法操作簡單,應用廣泛,可以擴展到電解液中的其他類似比率電化學傳感器的構建,這將有助于電化學傳感器的進一步發(fā)展。
[Abstract]:In recent years, the number of cancer patients has increased year by year due to the aging of population, the destruction of ecological environment, unhealthy lifestyle and other factors. Tumor chemotherapy has also made a considerable breakthrough, tumor patients survival time has been greatly extended. However, severe side effects caused by incomplete metabolism can also cause secondary injury. Therefore, drug treatment is limited to some extent. It is important to determine the concentration of residual drugs in body fluid, especially in blood samples. Electrochemical sensors have been a very active research frontier in analytical science because of their high sensitivity and good selectivity. With the research on the structure of the sensor, the ratio of electrochemical biosensor has attracted more and more attention of researchers. Based on the ratio electrochemical detection method, an electrochemical sensor was constructed for the detection of antitumor drugs such as adriamycin hydrochloride (DOX) and quercetin qu. Firstly, a ratio electrochemical biosensor based on bare glassy carbon electrode (GCE) was used to detect the antitumor drug DOX. Based on the high electrochemical activity of DOX and methylene blue MB, they were directly added into the electrolyte without any modification of the electrode between dox and GCE. MB is used as a reference signal. When the concentration of MB is kept constant, the electrical signal caused by DOX and MB is gradually enhanced with the increase of the amount of DOX, and the electrical signals of DOX and MB are detected by electrochemical workstation. The rate dual signal detection of DOX is fast and highly sensitive. The ratio method can obviously reduce the inherent systematic error and accidental error of the electrochemical sensor derived from the environmental and personal operating factors, thus significantly enhancing the reproducibility and stability of the experimental scheme. Secondly, based on the ratio detection method, we constructed a simple electrochemical sensor by using naked GCE as the signal sensing platform to detect the Qui in electrolyte sensitively. In addition, thionine th has a good linear relationship with [qu] as an internal reference electrochemical probe. The detection limit is as low as 3.1 nm. The ratio detection method is highly selective and sensitive to qu in the interference test of real food samples. Compared with previous studies, a wider detection range of 0-15 渭 m ~ (-1) has been obtained. The advantage of this method is that the electrochemical determination of qu can be carried out directly in electrolyte, which is similar to the widely accepted fluorescence sensing method in aqueous media. The method is simple in operation and widely used, and can be extended to the construction of other similar ratio electrochemical sensors in electrolyte, which will be helpful to the further development of electrochemical sensors.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.1;TP212.3;TQ460.72

【參考文獻】

相關期刊論文 前1條

1 Yuan-Yuan Yao;Long Zhang;Zi-Fei Wang;Jing-Kun Xu;Yang-Ping Wen;;Electrochemical determination of quercetin by self-assembled platinum nanoparticles/poly(hydroxymethylated-3,4-ethylenedioxylthiophene) nanocomposite modified glassy carbon electrode[J];Chinese Chemical Letters;2014年04期

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本文編號：1851202