本文選題：D + L-扁桃酸　； 參考：《云南師范大學(xué)》2016年碩士論文

【摘要】：本論文主要工作有以下幾個(gè)方面:1.簡要介紹了手性及手性分離的意義、手性分離方法、手性拆分的現(xiàn)實(shí)意義及纖維素在手性分離中的應(yīng)用。重點(diǎn)介紹了高效液相色譜手性固定相對(duì)手性化合物的拆分和膜分離技術(shù)。2.纖維素是天然的多糖,分子內(nèi)含有大量的羥基和手性位點(diǎn),以纖維素為膜材料,制備了纖維素膜,考察了纖維素濃度、原料液濃度、浸泡時(shí)間、浸泡溫度等因素對(duì)膜吸附性能的影響。實(shí)驗(yàn)結(jié)果表明:在最優(yōu)的實(shí)驗(yàn)條件下,該膜對(duì)D,L-扁桃酸表現(xiàn)出較好的手性吸附性能,e.e.%值達(dá)到48.9%。實(shí)驗(yàn)還制備了纖維素手性固相萃取劑,將其填充到固相萃取管中,對(duì)D,L-扁桃酸進(jìn)行萃取研究,并對(duì)固相萃取條件進(jìn)行優(yōu)化。纖維素固相萃取劑對(duì)D,L-扁桃酸的萃取也具有一定的手性選擇性。3.將纖維素溶解在DMAc-LiCl中形成鑄膜液,將其涂覆在3-氨丙基三乙氧基硅膠上,制備了纖維素手性柱,采用不同體積比的正己烷/異丙醇、甲醇/水、純甲醇、純水作為流動(dòng)相,用高效液相色譜拆分D,L-扁桃酸,得到一定的分離效果。將纖維素膜取代纖維素手性柱進(jìn)行膜色譜研究,實(shí)驗(yàn)表明纖維素手性膜色譜對(duì)D,L-扁桃酸也有一定的手性識(shí)別能力。4.將制備的纖維素膜,分別以濃度差和壓力差為膜過程驅(qū)動(dòng)力,考察了纖維素濃度、原料液濃度、滲過時(shí)間和流速不同時(shí),其對(duì)D,L-扁桃酸吸附效果的影響,在最優(yōu)的實(shí)驗(yàn)條件下,該膜對(duì)D,L-扁桃酸的手性拆分的e.e.%值分別達(dá)到70.1%和20.9%。
[Abstract]:The main work of this paper is as follows: 1. The significance of chirality and chiral separation, the method of chiral separation, the practical significance of chiral separation and the application of cellulose in chiral separation were briefly introduced. The resolution and membrane separation techniques of chiral stationary relative chiral compounds by high performance liquid chromatography (HPLC). Cellulose is a natural polysaccharide with a large number of hydroxyl groups and chiral sites in the molecule. Cellulose membrane was prepared with cellulose as the membrane material. The concentration of cellulose, the concentration of raw material solution and the soaking time were investigated. The influence of soaking temperature on the adsorption performance of the membrane. The experimental results show that under the optimum experimental conditions, the chiral adsorption property of the film for Dnlmandelic acid is 48.9%. Cellulose chiral solid phase extractant was also prepared and filled in a solid phase extraction tube to study the extraction of DL- mandelic acid. The conditions of solid phase extraction were optimized. Cellulose solid phase extractant also has chiral selectivity. Cellulose was dissolved in DMAc-LiCl to form a casting solution and coated on 3-aminopropyl triethoxy silica gel. The chiral cellulose column was prepared by using n-hexane / isopropanol, methanol / water, pure methanol and pure water as mobile phase. Dichlor-mandelic acid was separated by high performance liquid chromatography (HPLC). Cellulose membrane instead of cellulose chiral column was studied by membrane chromatography. The experimental results showed that cellulose chiral membrane chromatography also had a certain chiral recognition ability to DnL- mandelic acid. The effects of different concentration of cellulose, concentration of raw material liquid, permeation time and flow rate on the adsorption of DL- mandelic acid were investigated by using the concentration difference and pressure difference as the driving force of the membrane process, respectively, under the optimum experimental conditions, the effect of the cellulose membrane on the adsorption of DL- mandelic acid was studied under the optimum experimental conditions. The chiral resolution value of the membrane was 70.1% and 20.9%, respectively.
【學(xué)位授予單位】：云南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O636.11

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