本文選題：HPLC-DAD + 酸橙枳實(shí)　； 參考：《中國(guó)中藥雜志》2016年17期

【摘要】：采用HPLC-DAD方法建立18個(gè)批次酸橙枳實(shí)以及10個(gè)批次甜橙枳實(shí)的指紋圖譜,通過(guò)對(duì)照品對(duì)照和紫外圖譜指認(rèn)了17個(gè)色譜峰并對(duì)其進(jìn)行含量測(cè)定。色譜條件:Agilent Poroshell 120 SB-C18色譜柱(4.6 mm×100 mm,2.7μm),以流動(dòng)相甲醇-0.1%甲酸水溶液梯度洗脫,流速0.6 m L·min-1,檢測(cè)波長(zhǎng)318 nm,柱溫為30℃。采用中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)(2004A)軟件進(jìn)行指紋圖譜分析,進(jìn)一步通過(guò)SIMCA-P 13.0軟件對(duì)17個(gè)確認(rèn)的成分含量進(jìn)行最小二乘法判別分析,確定區(qū)分酸橙枳實(shí)與甜橙枳實(shí)的差異性標(biāo)記物。該方法具有良好的精密度、穩(wěn)定性和重復(fù)性,為枳實(shí)的質(zhì)量控制和評(píng)價(jià)提供依據(jù)。
[Abstract]:The fingerprint of 18 batches of Citrus aurantii and 10 batches of Citrus aurantii were established by HPLC-DAD. 17 chromatographic peaks were identified and determined by contrast and UV spectra. The chromatographic conditions were as follows: 1: Agilent Poroshell 120 SB-C18 column: 4.6 mm 脳 100mm ~ (2. 7) 渭 m ~ (-1), gradient elution with methanol-0.1% formic acid solution as mobile phase, flow rate 0.6 mL 路min ~ (-1), detection wavelength 318 nm, column temperature 30 鈩，

本文編號(hào)：1820456