本文選題：山姜屬 + 革葉山姜�。� 參考：《海南師范大學(xué)》2016年碩士論文

【摘要】：革葉山姜(Alpinia coriacea)為姜科(Zingiberaceae)山姜屬植物,是海南特有的藥用植物,通常生長于山地疏林中。山姜屬植物的果實、根莖等都具有很高的藥用價值,具有散寒燥濕、消炎止痛、暖胃止嘔等功效。該屬植物中的紅豆蔻、草豆蔻、益智以及高良姜等藥用植物被中國藥典收錄。截至目前為止尚未見任何關(guān)于革葉山姜化學(xué)成分及其藥理活性的研究報道。本課題組前期研究中發(fā)現(xiàn)革葉山姜的乙醇提取物具有顯著的生物活性,為了合理開發(fā)利用革葉山姜這一海南特有植物資源,篩選出具有較好活性的單體化合物,本課題組首次對革葉山姜進(jìn)行了化學(xué)成分和藥理活性的研究,為革葉山姜的合理開發(fā)與利用提供理論依據(jù)。本研究綜合運用硅膠柱層析、Sephadex LH-20柱層析、ODS柱層析、HPLC等色譜分離技術(shù)從革葉山姜的乙醇粗提物中分離得到30個化合物。利用多種波譜分析技術(shù)(如IR、UV、HRESI-MS、NMR等),結(jié)合與相關(guān)文獻(xiàn)數(shù)據(jù)比對,將它們分別鑒定為:alpicoriacine A(1),zedoalactone B(2),zedoalactone A(3),zedoarolide A(4),7-hydroxypiperitone(5),(E)-labda-8(17),12,14-trien-15(16)-olide(6),喬松素(7),芹菜素(8),良姜素(9),山奈酚(10),5,7,3′,4′-四羥基二氫黃酮(11),3,5-二羥基-7,4'-二甲氧基黃酮(12),槲皮素(13),5,7,3?,4?,5?-pentamethoxyflavone(14),對羥基苯甲醛(15),4-甲氧基苯甲酸(16),肉桂酸(17),丁香酸(18),丁香酸甲酯(19),3,4-二羥基苯甲酸(20),?-tocospiros A(21),6α-羥基豆甾-4-烯-3-酮(22),豆甾-4-烯-3-酮(23),7-酮基-β-谷甾醇(24),5α,8α-表二氧-(22E,24R)-麥角甾-6,22-二烯-3β-醇(25),豆甾醇(26),6β-羥基豆甾-4-烯-3-酮(27),豆甾-4-烯-3,6-二酮(28),β-谷甾醇(29)和β-胡蘿卜苷(30)。其中,化合物1為新化合物,化合物1-6為萜類化合物,化合物7-14為黃酮類化合物,化合物15-20為酚酸類化合物,化合物22-30為甾體類化合物�；衔�1-5、8、12、14、16、18-19、21-25和28為首次從山姜屬植物中分離得到;所有化合物均為首次從該植物中分離得到。抗菌活性測試結(jié)果表明化合物2、6、8、15和26對四聯(lián)球菌都顯示出一定的抑制活性,其中化合物2的MIC值為10μg/mL,其余MIC值均為20μg/mL。化合物2、4和25對大腸桿菌顯示出抑菌活性,MIC值為5μg/mL�；衔�25對金黃色葡萄球菌和枯草芽孢桿菌的MIC值均為10μg/mL�？鼓[瘤活性測試結(jié)果表明:化合物2、6和27對宮頸癌Hela細(xì)胞、人肝癌SMMC-7721細(xì)胞以及肺腺癌A-549細(xì)胞有一定的抑制活性,化合物23對宮頸癌Hela細(xì)胞有一定抑制活性,革葉山姜的揮發(fā)油對宮頸癌Hela細(xì)胞和肺腺癌A-549細(xì)胞有一定抑制活性。
[Abstract]:Alpinia coriacea is a plant of Zingiberaceae.Alpinia coriacea is a medicinal plant endemic to Hainan. The fruit, root and stem of Zingiber have high medicinal value, such as dispelling cold and dampness, eliminating inflammation and relieving pain, warming stomach and stopping vomiting and so on. The Chinese Pharmacopoeia includes red bean cardamom, cardamom, alpinia and alpinia. So far, no studies have been reported on the chemical constituents and pharmacological activities of Ginger. In our earlier study, we found that the ethanol extract of Ginger had significant biological activity. In order to develop and utilize Ginger, a unique plant resource in Hainan Province, the monomer compounds with good activity were screened out. The chemical constituents and pharmacological activities of Ginger were studied for the first time in order to provide theoretical basis for the rational development and utilization of Ginger. In this study, a total of 30 compounds were isolated from the crude ethanol extracts of Ginger by using silica gel column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography and other chromatographic techniques. By using various spectral analysis techniques (such as IRUVX HRESI-MSNMR, etc.), and comparing with the relevant literature data, 灝嗗畠浠垎鍒壌瀹氫負(fù):alpicoriacine A(1),zedoalactone B(2),zedoalactone A(3),zedoarolide A(4),7-hydroxypiperitone(5),(E)-labda-8(17),12,14-trien-15(16)-olide(6),涔旀澗绱，

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