本文選題：臭氧 + 熒光探針�。� 參考：《鄭州大學》2017年碩士論文

【摘要】：活性氧是自然界中普遍存在的一類化學性質非�；顫姷奈镔|。而臭氧(O_3)又是活性氧中最典型的一種。由于是三個氧原子組成,所以,人們在日常生活中狹義地把活性氧定義為臭氧。對人類來講,臭氧是一把雙刃劍,有益也有害。本論文首次報道了一種新型的用于檢測臭氧的有機小分子熒光探針。該小分子熒光探針在合成過程中表現(xiàn)出原料廉價,產率高,產物易于提純和化學性質穩(wěn)定等優(yōu)良性能�；谟袡C小分子熒光探針對環(huán)境、活體的適用性和對臭氧的選擇性響應,探針體系的熒光光譜會發(fā)生改變,從而實現(xiàn)對臭氧的檢測,并且成功將其用于活細胞的可視化熒光成像和環(huán)境中臭氧的半定量檢測。本論文主開展了以下方面的研究工作。首先,根據(jù)臭氧對丁-3-烯這一特定基團的識別,端位烯烴與臭氧反應通常經(jīng)歷臭氧環(huán)化和β-消除反應兩大步驟,其中β-消除反應是決速步驟,我們以此為主要出發(fā)點設計合成了一種以7-氨基-4-甲基香豆素為母體的用于檢測臭氧的熒光探針Cou-Bu。隨后我們在室溫條件下探究了該探針各種光譜性能,發(fā)現(xiàn)該熒光探針合成操作簡單,具有瞬間反應的特性,同時還表現(xiàn)出了靈敏度高、選擇性專一等優(yōu)點。其次,為了探索其生物應用價值,我們借助于熒光共聚焦顯微成像技術,把熒光探針Cou-Bu成功應用于活細胞中臭氧的可視化熒光成像�；罴毎麅韧瑫r存在的其它活性氧、抗氧化物質以及金屬離子也不對臭氧的檢測產生干擾。同時發(fā)現(xiàn)該探針表現(xiàn)出生物兼容性好,對細胞的損傷小等優(yōu)點。最后,我們還開展了環(huán)境中臭氧檢測相關方面的工作,制作了該探針的臭氧檢測試紙。常規(guī)的檢測環(huán)境中臭氧的方法只能達到定性的結果,我們的檢測試紙初步實現(xiàn)了半定量檢測環(huán)境中臭氧的目標。
[Abstract]:Reactive oxygen species (Ros) are one of the most active substances in nature. Ozone O _ 3) is one of the most typical reactive oxygen species. Reactive oxygen is defined as ozone in a narrow sense in daily life because it is made up of three oxygen atoms. For humans, ozone is a double-edged sword, beneficial and harmful. In this paper, a novel organic small molecule fluorescence probe for ozone detection is reported for the first time. The low molecular fluorescence probe showed excellent properties such as cheap raw materials, high yield, easy purification and stable chemical properties. Based on the applicability of organic small molecule fluorescence probe to environment, in vivo and the selective response to ozone, the fluorescence spectrum of the probe system will change, thus the ozone detection can be realized. It has been successfully used for visual fluorescence imaging of living cells and semi-quantitative detection of ozone in the environment. This thesis has carried out the following research work. First, based on the ozone recognition of a specific group of butadiene, the reaction of terminal olefin with ozone usually goes through two major steps: ozone cyclization and 尾 -elimination, in which the 尾 -elimination reaction is a critical step. We have designed and synthesized a fluorescent probe Cou-Bu-using 7-amino-4-methylcoumarin as the parent for the detection of ozone. Then we investigated the various spectral properties of the probe at room temperature. It was found that the fluorescent probe had the advantages of simple synthesis, instant reaction and high sensitivity and selectivity. Secondly, in order to explore its biological application value, we successfully applied fluorescent probe Cou-Bu to visualize fluorescence imaging of ozone in living cells by means of fluorescence confocal microscopy. Other reactive oxygen species, antioxidants and metal ions present in living cells do not interfere with ozone detection. At the same time, it was found that the probe had the advantages of good biological compatibility and less damage to cells. Finally, we also carried out the related work of ozone detection in the environment, and made the ozone detection paper of the probe. The conventional method of detecting ozone in the environment can only achieve qualitative results. Our test paper has initially achieved the goal of semi-quantitative detection of ozone in the environment.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3

【相似文獻】

相關期刊論文 前10條

1 王可;馬會民;;對生物環(huán)境因素敏感的熒光探針[J];化學進展;2010年08期

2 王姍姍;;小分子熒光探針在硫醇檢測中的最新研究進展[J];科技信息;2010年23期

3 向雨秘;龍少波;朱R，

本文編號：1785254