本文選題：銀納米粒子 + 動(dòng)力學(xué)�。� 參考：《青島科技大學(xué)》2017年碩士論文

【摘要】：銀納米材料因其自身具有獨(dú)特的物理與化學(xué)性能,在催化、抑菌、電學(xué)、光學(xué)等領(lǐng)域有著廣泛的應(yīng)用。銀納米粒子的性質(zhì)主要取決于它的形貌和尺寸,因此研究銀納米顆粒的可控制備具有重要的意義。在合適的入射光照射下,銀納米粒子表現(xiàn)出優(yōu)異的表面等離子體共振效應(yīng),這種共振效應(yīng)在熒光、拉曼信號(hào)增強(qiáng)方面有著廣泛的應(yīng)用。本文在傳統(tǒng)制備銀納米粒子方法的基礎(chǔ)上,通過(guò)優(yōu)化實(shí)驗(yàn)條件制備了球形、粒徑相對(duì)均一的銀納米粒子。在此基礎(chǔ)上利用銀納米粒子優(yōu)異的表面等離子共振增強(qiáng)熒光效應(yīng),實(shí)現(xiàn)對(duì)Cu~(2+)、焦磷酸根、凝血酶的靈敏檢測(cè)。本文主要進(jìn)行了以下三個(gè)方面的研究。1.在傳統(tǒng)檸檬酸鈉還原硝酸銀制備銀納米粒子的基礎(chǔ)上,研究了動(dòng)力學(xué)控制銀納米粒子生長(zhǎng)的影響因素。通過(guò)控制種子量、pH值以及溫度,可控得到30-157 nm的類(lèi)球形銀納米粒子。2.利用銀納米粒子表面等離子體共振增強(qiáng)熒光的特性,通過(guò)調(diào)控SiO_2殼層的厚度,實(shí)現(xiàn)Ag@SiO_2對(duì)CdS量子點(diǎn)熒光強(qiáng)度的最大增強(qiáng),實(shí)驗(yàn)表明SiO_2的厚度為14 nm時(shí),熒光強(qiáng)度達(dá)到2.3倍最大增強(qiáng)。利用CdS量子點(diǎn)對(duì)Cu~(2+)特異性選擇及PPi與Cu~(2+)之間具有的較強(qiáng)的結(jié)合能力,實(shí)現(xiàn)了對(duì)Cu~(2+)、焦磷酸根的靈敏檢測(cè)。3.利用銀納米粒子表面等離子體共振增強(qiáng)熒光的特性,通過(guò)DNA堿基互補(bǔ)配對(duì)的原則,將有機(jī)熒光染料Cy5帶到Ag@SiO_2表面,并探究了不同殼層厚度的Ag@SiO_2對(duì)熒光材料Cy5熒光強(qiáng)度的增強(qiáng)作用,最終實(shí)現(xiàn)了對(duì)Cy5熒光強(qiáng)度6.3倍的增強(qiáng)。利用凝血酶與適配體之間特異性結(jié)合以及氧化石墨烯與熒光材料Cy5之間的熒光共振能量轉(zhuǎn)移效應(yīng),實(shí)現(xiàn)了對(duì)凝血酶的靈敏檢測(cè)。
[Abstract]:Silver nanomaterials have been widely used in catalysis, bacteriostatic, electrical and optical fields because of their unique physical and chemical properties. The properties of silver nanoparticles mainly depend on their morphology and size, so it is of great significance to study the controllable preparation of silver nanoparticles. Silver nanoparticles exhibit excellent surface plasmon resonance (SPR) effect under appropriate incident light, which is widely used in fluorescence and Raman signal enhancement. Based on the traditional method of preparing silver nanoparticles, spherical and relatively homogeneous silver nanoparticles were prepared by optimizing the experimental conditions. On this basis, the excellent surface plasmon resonance enhanced fluorescence effect of silver nanoparticles was used to realize the sensitive detection of Cu~(2, pyrophosphate and thrombin. This paper mainly carries on the following three aspects of research. 1. Based on the reduction of silver nitrate by sodium citrate to silver nanoparticles, the factors influencing the growth of silver nanoparticles were studied. By controlling the amount of seed pH and temperature, the spherical silver nanoparticles of 30-157 nm were obtained. Using the characteristics of silver nanoparticles surface plasmon resonance enhanced fluorescence, the maximum fluorescence intensity of Ag@SiO_2 on CdS quantum dots is realized by adjusting the thickness of SiO_2 shell. The experimental results show that the thickness of SiO_2 is 14 nm. Fluorescence intensity reached 2.3 times maximum enhancement. The specific selection of Cu~(2 by CdS quantum dots and the strong binding ability between PPi and Cu~(2) were used to realize the sensitive detection of Cu~(2 and pyrophosphate. Based on the surface plasmon resonance enhanced fluorescence of silver nanoparticles and the principle of complementary matching of DNA bases, the organic fluorescent dye Cy5 was brought to the surface of Ag@SiO_2. The enhancement effect of Ag@SiO_2 with different shell thickness on the fluorescence intensity of Cy5 was investigated. Finally, the fluorescence intensity of Cy5 was enhanced by 6.3 times. The specific binding between thrombin and aptamer and the fluorescence resonance energy transfer effect between graphene oxide and fluorescent material Cy5 were used to detect thrombin sensitively.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TB383.1;O657.3

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本文編號(hào)：1781483