本文選題：甲胎蛋白 + 導電聚合物　； 參考：《石河子大學》2016年碩士論文

【摘要】：研究表明,目前唯一可以用來篩選和檢測腫瘤標志物的重要標志是人體血清中甲胎蛋白(AFP)的含量,及時發(fā)現(xiàn)與診斷腫瘤的出現(xiàn)可以準確且靈敏地檢測出血清中的AFP含量。傳統(tǒng)免疫監(jiān)測方法由于分析時間長、分析儀器昂貴及需要專業(yè)操作人員等限制了其發(fā)展,此時具有操作簡單、靈敏度高、測定時間較短及成本低等特點的免疫傳感器得到了研究者的強烈關注。本文根據(jù)不同的電極修飾材料制備了高靈敏度及工作性能穩(wěn)定的電化學免疫傳感器。電化學免疫傳感器中我們主要研究的是敏感界面電活性材料的修飾。在本文中我們應用的是不易在電極表面流失,易于固定在電極表面的導電高分子聚合物。導電聚合物目前在傳感器中廣泛應用主要是由于其優(yōu)良的性質(環(huán)境友好性、高電導率、穩(wěn)定性好等)。導電聚合物如聚苯胺(PANI)、聚吡咯(PPy)、聚鄰苯二胺(PoPD)等經過摻雜后導電性能有著很大的改善,利用此類型材料構建的電化學免疫傳感器的性能能夠得到很大的提升。最近幾年,仿生納米材料因其具有成膜性優(yōu)良、制備簡單及生物相容性好的特性,在免疫傳感器的研制中成為研究方向的著力點之一。聚多巴胺目前作為一類很好的二級反應平臺引起了人們的廣泛關注,這主要是由于聚多巴胺的結構中含有氨基和酚羥基,從而使其生物相容性良好,是目前眾多生物友好型大分子中的一種。本論文采用電聚合及電沉積方法將導電聚合物固定在電極上從而構建出一系列高度靈敏和穩(wěn)定性優(yōu)良的電化學免疫傳感器;試驗中采用電化學分析技術對免疫電極的自組裝過程和電極界面進行了表征并探討了免疫電極在生物分析方面的應用。本論文主要研究內容如下:1.基于摻雜態(tài)PANI構建的甲胎蛋白免疫傳感器:組裝完成了具有有高選擇性、穩(wěn)定性、靈敏性的新型非標記型電流型免疫傳感器,主要用于甲胎蛋白(AFP)的檢測。采用電聚合方法將聚苯胺薄膜修飾在玻碳電極表面,接著將甲胎蛋白抗體(anti-AFP)修飾在聚苯胺表面。實驗表明此免疫傳感器的線性范圍為0.01-50 ng/mL,檢出限可以達到0.008 ng/mL。此免疫傳感器在制備過程中體現(xiàn)出了選擇性高、重現(xiàn)性及穩(wěn)定性好的優(yōu)點。2.基于PPy及PDA構建的甲胎蛋白免疫傳感器:聚吡咯/聚多巴胺(PPy/PDA)非標記型電化學免疫傳感器的成功組裝,可以用于檢測甲胎蛋白(AFP)。采用電沉積方法將吡咯(Py)沉積在玻碳電極表面形成聚吡咯(PPy)膜,然后利用多巴胺(DA)在弱堿性溶液中可以自氧化聚合,在PPy表面包覆成膜修飾在電極表面以固定anti-AFP。聚多巴胺膜的優(yōu)良的生物相容性為該傳感器固定抗體數(shù)量的提高提供了有利條件。實驗表明此免疫傳感器的線性范圍為0.02-80 ng/mL,檢出限為0.01ng/mL。3.基于PANI及PDA構建的甲胎蛋白免疫傳感器:構建了基于聚苯胺/聚多巴胺(PANI/PDA)檢測甲胎蛋白抗原(AFP)的非標記電化學免疫傳感器。采用電聚合方法將聚苯胺(PANI)薄膜修飾在玻碳電極表面,然后利用多巴胺(DA)在弱堿性溶液中自聚合為PDA,在PANI表面包覆成膜修飾在電極表面以固定anti-AFP。對聚苯胺的摻雜改性拓寬了其pH適應范圍,提高了其在傳感器中的應用,此外,聚多巴胺薄膜表面富集了大量的氨基和羥基為該傳感器修飾蛋白提供里良好的生物環(huán)境。此免疫傳感器的線性范圍為0.01-80 ng/mL,檢出限為0.007 ng/mL。4.基于氮摻雜多孔碳及PoPD構建的甲胎蛋白免疫傳感器:利用氮摻雜多孔碳的良好的生物相容性提高了鄰苯二胺的電化學活性,增加了AFP的固載數(shù)量且保持蛋白的活性,加快電子傳遞,從而增加免疫傳感器的靈敏度。且聚鄰苯二胺表面含有很多氨基及亞氨基等,可以提供很多再生基團,為其固載anti-AFP提供了優(yōu)良條件。在優(yōu)化條件下,免疫傳感器的檢測范圍為0.005-100ng/mL,檢出限為0.0006 ng/mL。
[Abstract]:The research shows that the only important sign can be used to screening and detection of tumor markers is in human serum alpha fetoprotein (AFP) content, timely detection and diagnosis of tumor can accurately and sensitively detect hemorrhage AFP content in Qing Dynasty. The traditional immune monitoring method by analysis of long, expensive and needs analysis instruments the operation of professional personnel and limited its development. This has the advantages of simple operation, high sensitivity, immune sensor for determination of shorter and low cost has been a strong concern of the researchers. According to the different electrode modification material for electrochemical immunosensor with high sensitivity and stable performance were prepared. The electrochemical immunosensor in our main study on the modification is sensitive interface electrically active material. We used in this paper is not easy to be lost in the electrode surface, easy to be fixed on the electrode surface guide The electric conductive polymer. The polymer is widely used in the sensor is mainly due to its excellent properties (environmental friendliness, high conductivity, good stability etc.). Conductive polymers such as polyaniline (PANI), polypyrrole (PPy), poly benzene amine two (PoPD) after doping conductive performance has greatly improved the performance of electrochemical immunosensor, this type of material can be greatly improved. In recent years, biomimetic nano materials because of its excellent film-forming properties, simple preparation and characteristics: good biocompatibility, become the focus of research in the development of immune sensor. Two order reaction platform polydopamine at present, as a kind of good has aroused extensive attention, this is mainly due to the structure of poly dopamine containing amino and phenolic hydroxyl groups, making it a good biocompatibility, is the current number of bio friendly A type of large molecules. This paper uses electro polymerization and method of electro deposition of conductive polymer immobilized on the electrode in order to construct a series of highly sensitive stability and excellent electrochemical immunosensor; test by electrochemical analysis technique on immune electrode self-assembly process and electrode interface were characterized and discussed immunoelectrode application in biological analysis. The main contents of this dissertation are as follows: 1. AFP immunosensor based on PANI doped: assembled with high selectivity, stability, novel label free amperometric immunosensor sensitivity, mainly used for alpha fetoprotein (AFP) detection. Polymerization of polyaniline films modified on the surface of glassy carbon electrode using electricity, then the alpha fetoprotein antibody (anti-AFP) in surface modification of polyaniline. The experiment shows that the immune sensor linear range 0.01-50 ng/mL, the detection limit can reach 0.008 ng/mL. the immune sensor in the preparation process showed high selectivity, reproducibility and stability of the advantages of.2. based on the AFP immunosensor of PPy and PDA Construction: polypyrrole / polydopamine (PPy/PDA) label free electrochemical immunosensor successfully assembled, can be used for the detection of alpha fetoprotein (AFP). By using the method of electro deposition of pyrrole (Py) deposited on the surface of glassy carbon electrode polypyrrole (PPy) film, and then use the dopamine (DA) can self oxidation polymerization in alkaline solution, the PPy coating film on the electrode surface modified with fixed anti-AFP. polydopamine film excellent biocompatibility provides favorable conditions for the number of sensor fixing antibodies increased. Experiments show that the linear range of the immunosensor for 0.02-80 ng/mL, the detection limit is 0.01ng/mL.3. based on PANI and PDA construction AFP immunosensor: Construction of the polyaniline / poly dopamine (PANI/PDA) based on the detection of alpha fetoprotein (AFP) antigen label free electrochemical immunosensor. Polymerization method using electric polyaniline (PANI) film modified on glassy carbon electrode surface, and then use the dopamine (DA) in alkaline solution to self polymerization of PDA. On the surface of PANI film coated on the electrode surface modified with anti-AFP. fixation of polyaniline doped pH to broaden its scope, improve its application in sensors, in addition, the polydopamine film surface enrichment of a large number of amino and hydroxyl groups for the sensor protein modification of good environment. The linear range of the organisms immune the sensor is 0.01-80 ng/mL, a detection limit of 0.007 ng/mL.4. AFP immunosensor based on nitrogen doped porous carbon and PoPD Construction: the use of nitrogen doped porous carbon with good biocompatibility To improve the electrochemical activity of benzene amine two, increase the number of supported and maintained the activity of the protein AFP, accelerate the electron transfer, thereby increasing the sensitivity of the immunosensor. And the poly phthalate two amine surface contains many amino and imino groups, can provide a lot of regeneration, provides excellent conditions for the immobilization of anti-AFP. Under the optimized conditions, the detection range of immune sensor is 0.005-100ng/mL, the detection limit was 0.0006 ng/mL.

【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：O657.1

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