本文選題：化妝品　切入點(diǎn)：美白劑　出處：《分析測試學(xué)報》2017年03期

【摘要】：建立了同時測定化妝品中10種美白活性成分及2種禁用成分的高效液相色譜分析方法。水基、乳液等含油脂較少的樣品采用0.02 mol/L磷酸二氫鉀溶液(pH 6.0)直接提取;油脂含量高的樣品及蠟基、粉基類的樣品先加入2.5 m L二氯甲烷溶解后再用0.02 mol/L磷酸二氫鉀溶液(pH 6.0)提取。提取液在9 500r/min下離心后用0.22μm濾膜過濾。樣品采用Eclipse XDB-C_(18)色譜柱為固定相,以0.02 mol/L磷酸二氫鉀溶液(pH 6.0)和甲醇溶液為流動相,梯度洗脫,流速為1.0 m L/min,柱溫為25℃,使用二極管陣列檢測器(DAD)進(jìn)行檢測,檢測波長為230 nm和250 nm,外標(biāo)法定量。結(jié)果顯示:12種化合物在2.5~100 mg/L范圍內(nèi)線性關(guān)系良好,相關(guān)系數(shù)(r)均大于0.999 0。方法的定量下限(以信噪比為10計)為0.006 5%~0.025%,添加水平為0.025%~0.5%時回收率為87%~102%,相對標(biāo)準(zhǔn)偏差均小于4%。該方法前處理簡單、回收率高、精密度好,適用于化妝品中10種美白活性成分及2種禁用成分的快速測定。
[Abstract]:A high performance liquid chromatography ( HPLC ) method for simultaneous determination of 10 kinds of whitening active components and two banned components in cosmetics was established . The samples with low oil content were directly extracted with 0.02 mol / L potassium dihydrogen phosphate solution ( pH 6.0 ) . The samples with high fat content were separated by using 0.026mol / L potassium dihydrogen phosphate solution ( pH 6.0 ) . The results showed that the recoveries were 87 % 锝，

本文編號：1726674