本文選題：SERS　切入點(diǎn)：柔性　出處：《江蘇科技大學(xué)》2017年碩士論文

【摘要】：表面增強(qiáng)拉曼光譜(Surface enhanced Raman spectroscopy,SERS)自發(fā)現(xiàn)以來就備受關(guān)注,隨著激光技術(shù)等技術(shù)發(fā)展,SERS技術(shù)的不斷進(jìn)步,該技術(shù)已經(jīng)發(fā)展到用來定量檢測(cè)的階段,而且圍繞該技術(shù)也產(chǎn)生了很多的新型基底模型。但是,SERS技術(shù)在普及性上還沒有得到廣泛的應(yīng)用。論文中通過采用不同的制備方法得到了四種不同類型的基底,同時(shí)對(duì)基底各方面的的性能也進(jìn)行了比較詳細(xì)的表征與研究。主要的研究?jī)?nèi)容與結(jié)果如下:1.利用聚多巴胺(PDA)作為二級(jí)反應(yīng)平臺(tái)和原位還原劑,以銀納米顆粒(AgNPs)為基礎(chǔ)制備Si/PDA/AgNPs基底。該基底表面均勻,有非常良好的信號(hào)重現(xiàn)性,各個(gè)特征峰的峰強(qiáng)的相對(duì)標(biāo)準(zhǔn)偏差(RSD)值都在10%以內(nèi),遠(yuǎn)低于20%的常規(guī)值。基底所能測(cè)量到的羅丹明6G(R6G)的最低濃度達(dá)到了10~(-8)M,增強(qiáng)因子(EF)達(dá)到了3.6×106。該方案在制備基底的過程中,充分利用了PDA的特性,制備過程中沒有使用其它的還原劑,制備的步驟簡(jiǎn)單,反應(yīng)過程綠色環(huán)保。解決了不少基底制備過程中的,還原復(fù)雜、有污染的弊端,在制備工藝的理念上更加的先進(jìn)。2.實(shí)驗(yàn)在第一個(gè)體系的基礎(chǔ)上,制備PDA/AgNPs/PDA型“三明治”結(jié)構(gòu)基底。實(shí)驗(yàn)中PDA不但被當(dāng)作二級(jí)反應(yīng)平臺(tái),還被作為還原劑和保護(hù)膜使用。該“三明治”結(jié)構(gòu)基底有很長(zhǎng)的保存時(shí)間,在測(cè)試初期,由于PDA保護(hù)膜的覆蓋,信號(hào)強(qiáng)度出現(xiàn)了一定的衰減,但衰減未達(dá)到一個(gè)數(shù)量級(jí)�！叭髦巍苯Y(jié)構(gòu)基底在后續(xù)測(cè)試中信號(hào)強(qiáng)度比普通基底更強(qiáng)更穩(wěn)定,作為對(duì)照組的普通活性基底信號(hào)強(qiáng)度則隨著時(shí)間的推移,呈現(xiàn)一種遞減趨勢(shì)。而且,“三明治”結(jié)構(gòu)基底在六個(gè)月后極限時(shí)間測(cè)試時(shí)還有信號(hào)。作為對(duì)照,普通基底已無(wú)法檢測(cè)到R6G的信號(hào)。在惡劣紫外光環(huán)境下的測(cè)試結(jié)果也表明:其性能即使在較長(zhǎng)時(shí)間紫外光照射后基底依然有信號(hào),相比與普通活性基底優(yōu)勢(shì)明顯。3.論文中還進(jìn)行了柔性SERS基底的開發(fā),利用聚對(duì)苯二甲酸乙二醇酯(PET)和聚酯纖維布兩種比較常見而且價(jià)格低廉的原材料進(jìn)行基底的制作,制備的基底能測(cè)量到的R6G極限濃度也達(dá)到了10-8M,基底的重現(xiàn)性也很好,基底非常的均勻。而且基底在經(jīng)過100次彎折后,在測(cè)試中信號(hào)強(qiáng)度幾乎沒有變化,說明柔性基底的抗性非常好。4.最后,方案設(shè)計(jì)核殼結(jié)構(gòu)模型的SERS基底,與銀溶膠類基底類似,實(shí)驗(yàn)中采用納米銀顆粒包裹二氧化硅以及用PDA包裹納米銀粒子兩種方案,對(duì)兩種基底的穩(wěn)定性能進(jìn)行研究。對(duì)比普通銀膠基底,兩種基底在性能保存時(shí)間上都有較大優(yōu)勢(shì)。由于在測(cè)試中在探針分子溶液的滴加上與平面或固體基底不同,所以表征中不再計(jì)算EF,而且由于采用核殼結(jié)構(gòu)的目的就是對(duì)基底進(jìn)行保護(hù),從測(cè)試結(jié)果中可以看出,該模型完全達(dá)到了設(shè)計(jì)要求。論文中制備了四類活性基底,并且研究了基底的各種性能,發(fā)現(xiàn)基底在較長(zhǎng)時(shí)間內(nèi)都能保持性能的穩(wěn)定,這使得它的應(yīng)用前景變得更加多樣。而且,由于其具有較長(zhǎng)的“保質(zhì)期”,所以其具有成為更加普及的消費(fèi)品的潛力。
[Abstract]:Surface enhanced Raman spectroscopy (Surface enhanced Raman spectroscopy, SERS) has attracted much attention since it was discovered, along with the development of laser technology, the development of SERS technology, this technology has been used to develop quantitative detection stage, and around it also generate a new substrate model a lot. However, SERS technology has not been widely the application in the popularization of the thesis. By using different preparation methods and obtained four kinds of substrates of different types, and the performance of all aspects of the substrate were also characterized and studied in detail. The main research contents and results are as follows: 1. using polydopamine (PDA) as the two order reaction platform and in situ reduction agent, with silver nanoparticles (AgNPs) as the basis for preparing Si/PDA/AgNPs substrate. The substrate surface is uniform, the signal is very good reproducibility and the relative standard of each feature of the peak of strong The standard deviation (RSD) values are less than 10%, far lower than the conventional value of 20%. The substrate can be measured by the Luo Danming 6G (R6G) is the lowest concentration reached 10~ (-8) M, the enhancement factor (EF) reached 3.6 * 106. the preparation process of substrate in the system, make full use of the PDA the characteristics, preparation process without the use of other reducing agent, the preparation procedure is simple, green reaction process. To solve a lot of basement in the preparation process, reducing complexity, drawbacks of pollution, in the preparation process of the concept of more advanced.2. experiment based on the first system, system preparation of PDA/AgNPs/PDA type sandwich structure. The PDA substrate was not only as a two order reaction platform, also as a reducing agent and the use of the protective film. The "sandwich" structure of substrate has a long storage time, at the beginning of the test, the PDA protective film covering, there was a certain signal strength attenuation, But the attenuation did not reach a magnitude. The signal intensity of basal sandwich structure in the subsequent test is more stable than ordinary basement is stronger, as a common substrate signal strength in the control group with the passage of time, showing a decreasing trend. Moreover, the "basal sandwich structure" in the six months after the time limit test there was a signal. As a control, a common base has been unable to detect the R6G signal. In the harsh environment of the UV test results show that the performance even in a long time after ultraviolet irradiation is still a basal signal, compared with the ordinary active substrate have obvious advantages in the paper of.3. also developed flexible SERS substrate, using polyethylene terephthalate glycol ester (PET) and polyester fiber cloth made of two kinds of raw material is relatively common and low price of the substrate, the R6G limit of the prepared substrate can be measured by the concentration also At 10-8M, the basal reproducibility is also very good, very uniform. And the basal substrate after 100 after bending, in the test signal intensity is almost no change resistant flexible substrate is very good.4. finally, SERS base design core-shell structure model, similar to the silver sol substrate class, experiment the silver nanoparticles coated silica and silver nanoparticles coated by PDA two solution, to study stability of two kinds of substrate. Compared with common silver colloids, two kinds of substrate have great advantages in performance. Due to save time in the test on the probe molecule solutions adding on substrate and plane or solid different, so no longer in the characterization of EF is calculated, and the core-shell structure is to protect the substrate from the test results it can be seen that the model meets the design requirements. The preparation of four papers Type of substrate, and the study of the various properties of the substrate, the substrate that can maintain the performance in a relatively long period of stability, which makes its application become more diverse. But, because of its long shelf life, so it has become a more popular consumer potential.

【學(xué)位授予單位】：江蘇科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.37

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