本文選題：槲皮素　切入點：熒光探針　出處：《光譜學與光譜分析》2016年11期

【摘要】：槲皮素為天然黃酮類化合物,廣泛存在于植物的根、莖、葉、花和果實中。槲皮素作為熒光探針檢測氟離子不僅具有較好的選擇性和靈敏度,而且與合成的熒光探針比,還具有來源廣、環(huán)保、無毒等優(yōu)點。實驗將不同陰離子(F~-,Cl~-,Br~-,I~-,ClO_4~-,H_2PO_4~-)分別加入到槲皮素的二甲基亞砜(DMSO)溶液中,考查槲皮素溶液的熒光強度變化。實驗發(fā)現(xiàn)當加入氟離子后,槲皮素在500nm處的熒光發(fā)射峰的強度降低,發(fā)生熒光猝滅,且其猝滅程度隨著氟離子濃度的增大而改變,即熒光強度隨著氟離子濃度的增大而減小,并呈線性變化。而其他陰離子的加入對槲皮素和槲皮素-氟離子體系的熒光發(fā)射強度影響不大,說明其他陰離子不影響槲皮素對氟離子的識別,顯示了槲皮素對氟離子具有較好的選擇性。由熒光滴定光譜和熒光滴定曲線得到槲皮素對氟離子的滴定方程為:y=-13.36x+173.4,線性關系為R2=0.991,線性范圍為1.0×10~(-6)~8.0×10~(-6)mol·L~(-1),最低檢測限為1.0×10-7 mol·L~(-1),表明槲皮素對氟離子的識別具有較高的靈敏度。進一步實驗表明槲皮素識別氟離子的機理可能是氟離子的加入破壞了溶液體系的氫鍵,改變了槲皮素分子的共軛狀態(tài),發(fā)生分子內電荷轉移,促使槲皮素熒光猝滅。用該法成功檢測了樣品中微量氟離子,回收率為100.67%~102.44%,精確度較好,測定結果穩(wěn)定。
[Abstract]:Quercetin, a natural flavonoid, is widely found in the roots, stems, leaves, flowers and fruits of plants. Quercetin as a fluorescent probe not only has good selectivity and sensitivity, but also has better sensitivity than the synthetic fluorescent probe. It also has the advantages of wide source, environmental protection, non-toxicity and so on. In the experiment, different anionic anions were added to the dimethyl sulfoxide dimethyl sulfoxide (DMSOO) solution, and the fluorescence intensity of the quercetin solution was examined. It was found that the fluorescence intensity of the quercetin solution was changed with the addition of fluorine ion, and the different anions were added to the dimethyl sulfoxide dimethyl sulfoxide dimethyl sulfoxide (DMSO) solution. The intensity of fluorescence emission peak of quercetin at 500nm decreases, fluorescence quenching occurs, and the quenching degree changes with the increase of fluoride concentration, that is, the fluorescence intensity decreases with the increase of fluoride concentration. The addition of other anions had little effect on the fluorescence emission intensity of quercetin and quercetin-fluorine ion system, indicating that other anions did not affect the recognition of fluorine ions by quercetin. The results show that quercetin has good selectivity for fluorine ion, the titration equation of quercetin for fluorine ion is: yyyz-13.36x 173.4, the linear relation is R2O0.991.The linear range is 1.0 脳 1010-7 mol / L ~ (-1), the detection limit is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1), the linear range is 1.0 脳 10 ~ (-7) mol / L ~ (-1). The results show that quercetin has a high sensitivity to the recognition of fluorine ions. Further experiments show that the mechanism of quercetin recognition of fluorine ions may be that the addition of fluorine ions destroys the hydrogen bonds of the solution system. The conjugated state of quercetin molecule was changed and the intramolecular charge transfer occurred, which promoted the fluorescence quenching of quercetin. The method was used to determine trace fluorine ion in the sample successfully. The recovery rate was 100.677.The precision was good and the determination result was stable.
【作者單位】： 南京林業(yè)大學化學工程學院;南京林業(yè)大學南方現(xiàn)代林業(yè)協(xié)同創(chuàng)新中心;南京林業(yè)大學理學院;南京林業(yè)大學現(xiàn)代分析測試中心;南京林業(yè)大學林學院;
【基金】：國家林業(yè)局林業(yè)公益性行業(yè)科研專項項目(20120460102) 江蘇省第四期“333高層次人才培養(yǎng)工程”科研項目(BRA2012171) 國家科技支撐計劃課題項目(2012BAD21B04) 南京林業(yè)大學優(yōu)秀博士學位論文創(chuàng)新基金項目資助
【分類號】：O657.3

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