本文選題：DNA修飾電極　切入點：電化學(xué)傳感器　出處：《高等學(xué)校化學(xué)學(xué)報》2016年07期

【摘要】：利用銅離子(Cu~(2+))可與DNA分子中的堿基相互作用形成絡(luò)合物的性質(zhì),將Cu~(2+)富集在DNA修飾電極表面,進而采用微分脈沖伏安法(DPV)實現(xiàn)了銅離子的檢測.此外,由于乙二胺四乙酸(EDTA)對Cu~(2+)具有更強的絡(luò)合能力,富集于DNA修飾電極表面的Cu~(2+)很容易被洗脫液中的EDTA絡(luò)合,從而實現(xiàn)修飾電極的再生和重復(fù)利用.實驗結(jié)果表明,在最佳實驗條件下,Cu~(2+)濃度在2.0×10-6~1.0×10-5mol/L和2.0×10-5~1.0×10-4mol/L范圍內(nèi)與其相對還原峰電流強度(I-I0)呈良好的線性關(guān)系,且該傳感器簡單、穩(wěn)定,可循環(huán)使用.
[Abstract]:The copper ion was determined by differential pulse voltammetry (DPV). The copper ion can interact with the base group in the DNA molecule to form a complex. The Cu~(2) is enriched on the surface of the DNA modified electrode, and then the copper ion is detected by differential pulse voltammetry. Because ethylenediamine tetraacetic acid (EDTA) has stronger complexing ability to Cu~(2, Cu~(2 enriched on the surface of DNA modified electrode is easy to be complexed with EDTA in eluent, thus the modified electrode can be regenerated and reused. Under the optimum experimental conditions, the relative reduction peak current intensity (I-I0) of the sensor is linear in the range of 2.0 脳 10 ~ (-6) 10-5mol/L and 2.0 脳 10 ~ (-5) 10-4mol/L, and the sensor is simple, stable and recyclable.
【作者單位】： 陜西師范大學(xué)化學(xué)化工學(xué)院陜西省生命分析化學(xué)重點實驗室;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號:21575085)資助~~
【分類號】：O657.1

【相似文獻】

相關(guān)期刊論文 前10條

1 陳繪麗 ,楊頻;A Novel Cobalt(III) Mixed-polypyridyl Complex: Synthesis, Characterization and DNA Binding[J];Chinese Journal of Chemistry;2002年12期

2 陳婧,康敬萬;Interaction between Eu(bpy)_3~(3+) Complex and DNA by Fluorophotometry[J];Journal of Rare Earths;2003年S1期

3 何忠效,顧郁;ENHANCEMENT OF THE SALT EFFECT ON THE CONFORMATION OF M. L. DNA MOLECULES BY METHYLATION[J];Science in China,Ser.B;1990年06期

4 ;STUDIES ON THE SYNTHESIS AND DNA-BINDING ABILITY OF ZINC FINGER MOTIF[J];Chinese Chemical Letters;1994年12期

5 張志剛,楊頻;二茂鈦二甘氨酸鹽酸鹽與DNA作用機理的研究[J];高等學(xué)�；瘜W(xué)學(xué)報;1997年08期

6 ;Regulation of Cleavage Activity of Ser-His Dipeptide on DNA by Phosphorylation[J];Chinese Chemical Letters;1997年07期

7 ;A Special Property of DNA--as an Electrocatalyst[J];Chinese Chemical Letters;1998年03期

8 倪京滿,楊曉武,沈健恒,賈正平,王銳;Synthesis, Conformation, Biological Activity and DNA-Binding Characteristics of Antitumor Drug Actinomycin D Analogs[J];廈門大學(xué)學(xué)報(自然科學(xué)版);1999年S1期

9 戴偉民,吳安心;Allylic Rearrangement Strategy for in situ Formation of Potent DNA-Cleaving 10-Membered Ring Enediynes[J];廈門大學(xué)學(xué)報(自然科學(xué)版);1999年S1期

10 AlbertS.C.Chan,K-LDanielLee;Study on the Design and Synthesis of DNARecognition Molecule - Polyamide[J];廈門大學(xué)學(xué)報(自然科學(xué)版);1999年S1期

相關(guān)會議論文 前10條

1 韓璐;劉r，

本文編號：1674493