本文選題：光電化學(xué)　切入點(diǎn)：免疫傳感　出處：《南京大學(xué)》2016年碩士論文

【摘要】：光電化學(xué)生物傳感是將光電化學(xué)過(guò)程與生物分子識(shí)別過(guò)程相結(jié)合而發(fā)展起來(lái)的一種新的傳感技術(shù)。其檢測(cè)原理是：光照下識(shí)別元件和目標(biāo)分子之間的生物識(shí)別作用而產(chǎn)生相應(yīng)電信號(hào)的改變。相比較于傳統(tǒng)的光學(xué)方法,由于其具有裝置簡(jiǎn)單、價(jià)格低廉、易于微型化、背景信號(hào)低、靈敏度高等優(yōu)點(diǎn),光電化學(xué)生物傳感受到了越來(lái)越多的關(guān)注。最近十幾年來(lái),各種類型的傳感模式相繼出現(xiàn),多種目標(biāo)分析物如生物標(biāo)記物、DNA序列、細(xì)胞及其他生物分子被成功地檢測(cè)。本論文采用標(biāo)記性策略構(gòu)建了高靈敏的光電化學(xué)免疫傳感器,主要內(nèi)容如下：1.基于CdSe@ZnS敏化TiO2NWs/Au復(fù)合結(jié)構(gòu)及Ab2@V2+信號(hào)放大的光電化學(xué)免疫傳感對(duì)CA1 9-9的高靈敏檢測(cè)利用水熱法制備了Ti02納米線(TiO2NWs)。將TiO2NWs懸浮液滴涂在ITO電極上,高溫?zé)Y(jié)后形成一層致密的膜。將Au納米顆粒沉積到TiO2NWs表面形成TiO2NWs/Au復(fù)合結(jié)構(gòu)。利用層層組裝方法將CdSe@ZnS核殼量子點(diǎn)修飾于電極表面,形成TiO2NWs/Au/CdSe@ZnS敏化結(jié)構(gòu),并作為傳感電極基底用來(lái)固定CA19-9捕獲抗體(Abl)。將吡啶分子(V2+)標(biāo)記在CA19-9信號(hào)抗體(Ab2)上形成Ab2@V2+連接物,用作為信號(hào)放大元件。由于TiO2NWs/Au/CdSe@ZnS敏化結(jié)構(gòu)優(yōu)越的光電化學(xué)性能和Ab2@V2+連接物顯著的信號(hào)放大作用,所構(gòu)建的光電化學(xué)免疫傳感器顯示了對(duì)CA19-9的高靈敏檢測(cè),同時(shí)具有良好的特異性、重復(fù)性及穩(wěn)定性。本工作構(gòu)建的光電化學(xué)平臺(tái)適用于各種類型的高靈敏光電化學(xué)免疫分析法,尤其針對(duì)于疾病相關(guān)的生物標(biāo)記物的微量或痕量測(cè)定。2.基于CdSeTe@CdS:Mn核殼量子點(diǎn)敏化Ti02及CuS納米晶標(biāo)記的增強(qiáng)型光電化學(xué)夾心免疫傳感首先將Ti02納米顆粒懸浮液滴涂在ITO電極上,高溫?zé)Y(jié)后形成一層致密的膜。采用靜電吸附方法將CdSeTe三元合金量子點(diǎn)(AQDs)修飾于ITO/TiO2電極上,繼而通過(guò)連續(xù)離子層吸附與反應(yīng)技術(shù)將CdS:Mn量子點(diǎn)沉積于CdSeTe AQDs表面,形成TiO2/CdSeTe@CdS:Mn敏化結(jié)構(gòu),并作為傳感電極光電化學(xué)基底用于固定CEA捕獲抗體(Ab1)；CEA信號(hào)抗體(Ab2)用CuS納米晶(NCs)標(biāo)記,形成Ab2-CuS連接物,作為傳感器的信號(hào)放大元件。由于TiO2/CdSeTe@CdS:Mn敏化結(jié)構(gòu)優(yōu)越的光電化學(xué)性能及Ab2-CuS連接物顯著的信號(hào)放大作用,所設(shè)計(jì)的光電化學(xué)夾心免疫傳感器顯示了對(duì)目標(biāo)抗原的高靈敏檢測(cè),同時(shí)還表現(xiàn)了良好的重現(xiàn)性、特異性及穩(wěn)定性,為光電化學(xué)免疫分析提供了一種新型的構(gòu)建平臺(tái)。
[Abstract]:Photochemical biosensor is a new sensing technology which combines photochemical process with biomolecular recognition process. In contrast to traditional optical methods, Because of its advantages of simple device, low price, easy miniaturization, low background signal and high sensitivity, photochemical biological transmission has attracted more and more attention. A variety of target analytes such as biomarkers such as DNA sequences, cells and other biomolecules have been successfully detected. In this paper, a highly sensitive photochemical immunosensor was constructed using labeling strategies. The main contents are as follows: 1.The high sensitivity detection of CA1 9-9 by photochemical immunosensor based on CdSe@ZnS sensitized TiO2NWs/Au composite structure and Ab2@V2 signal amplification. The Ti02 nanowires TiO2NWsN were prepared by hydrothermal method. The TiO2NWs suspension droplet was coated on the ITO electrode. After sintering at high temperature, a dense film was formed. Au nanoparticles were deposited on the surface of TiO2NWs to form TiO2NWs/Au composite structure. CdSe@ZnS core-shell quantum dots were modified on the electrode surface by layer-by-layer assembly method to form TiO2NWs/Au/CdSe@ZnS sensitized structure. It was used as the substrate of the sensing electrode to immobilize the CA19-9 capture antibody, and the pyridine molecule V2 was labeled on the CA19-9 signal antibody (Ab2) to form a Ab2@V2 junction. Because of the superior photochemical properties of TiO2NWs/Au/CdSe@ZnS sensitized structure and the remarkable signal amplification effect of Ab2@V2 connectors, the photochemical immunosensor developed in this paper shows a high sensitivity to CA19-9 detection and has good specificity. Reproducibility and stability. The photochemical platform constructed in this work is suitable for various types of highly sensitive photochemical immunoassay. Especially for the trace or trace determination of disease-related biomarkers. (2) enhanced photoelectrochemical sandwich immunosensors based on CdSeTe@CdS:Mn core-shell quantum dots sensitized Ti02 and CuS nanocrystalline labeling first coated Ti02 nanoparticles suspension droplets on ITO electrodes. A dense film was formed after sintering at high temperature. The CdSeTe ternary alloy quantum dots were modified on the ITO/TiO2 electrode by electrostatic adsorption, and then the CdS:Mn quantum dots were deposited on the surface of CdSeTe AQDs by continuous ion layer adsorption and reaction technique, and the TiO2/CdSeTe@CdS:Mn sensitized structure was formed. It was used as the photochemical substrate of the sensing electrode for immobilization of CEA capture antibody (Ab1) and CEA signal antibody (Ab2) labeled with CuS nanocrystalline (NCs) to form a Ab2-CuS junction. As the signal amplifying element of the sensor, because of the superior photoelectric chemical property of the TiO2/CdSeTe@CdS:Mn sensitized structure and the remarkable signal amplification effect of the Ab2-CuS connector, the photoelectric chemical sandwich immunosensor designed by the sensor shows a high sensitivity to the detection of the target antigen. At the same time, it also shows good reproducibility, specificity and stability, which provides a new construction platform for photochemical immunoassay.
【學(xué)位授予單位】：南京大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O657.1;TP212

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本文編號(hào)：1669087