本文選題：手性　切入點(diǎn)：酒石酸　出處：《西南交通大學(xué)》2017年碩士論文

【摘要】：手性是生命體系的基本特征,構(gòu)成生命體的有機(jī)分子絕大多數(shù)是手性分子,并表現(xiàn)出高度的手性選擇,因此不同的手性分子與生物體系相互作用會(huì)表現(xiàn)出不同的生物學(xué)功能。本論文首先通過(guò)聚多巴胺和己二胺在Ti-O膜表面構(gòu)建不同的手性體系,即固定手性酒石酸體系和固定手性酒石酸鈉體系,對(duì)手性體系進(jìn)行篩選,隨后對(duì)篩選出的手性表面進(jìn)行纖維蛋白原變性和血小板粘附與激活實(shí)驗(yàn)評(píng)價(jià),考察手性表面的血液相容性。硒代胱胺分子中二硒鍵具有催化內(nèi)源性一氧化氮供體釋放一氧化氮的能力,因此本論文還將硒代胱胺取代己二胺,在材料次表面引入催化活性中心,最后在材料的最表面接枝手性酒石酸,考察不同手性酒石酸的固定對(duì)次表面硒代胱胺催化供體釋放一氧化氮的影響以及隨后對(duì)血小板粘附與激活的影響。X射線(xiàn)光電子能譜(XPS)、水接觸角測(cè)量(WCA)等方法對(duì)改性后的樣品進(jìn)行材料學(xué)的表征,結(jié)果表明沉積聚多巴胺的表面新出現(xiàn)N1s峰,而Ti-O膜上的Ti2p峰被完全遮蓋,說(shuō)明聚多巴胺薄膜制備成功。接枝己二胺樣品表面的N:C比例升高是因?yàn)橐肓舜罅康陌被?表明己二胺接枝成功,隨后接枝手性酒石酸和手性酒石酸鈉的樣品表面O:C比例升高表明手性酒石酸和手性酒石酸鈉接枝成功。WCA結(jié)果顯示,在材料表面固定同一系列手性分子,水接觸角幾乎沒(méi)有改變說(shuō)明表面手性不影響材料表面的親疏水性;利用QCM-D方法實(shí)時(shí)監(jiān)測(cè)各樣品對(duì)血漿蛋白如纖維蛋白原、牛血清白蛋白的吸附情況,結(jié)果直接證明樣品表面接枝的L-酒石酸和D-酒石酸數(shù)量無(wú)明顯差異,但隨后接枝D-酒石酸的樣品表面吸附纖維蛋白原量和牛血清白蛋白量顯著多于接枝L-酒石酸的樣品表面吸附的纖維蛋白原量和牛血清白蛋白量,固定D-酒石酸的樣品對(duì)血漿蛋白顯示出更好的親和性,這表明表面手性對(duì)蛋白吸附行為有著不可忽視的影響。而對(duì)于固定L-酒石酸鈉樣品來(lái)說(shuō),結(jié)果同樣顯示固定D-酒石酸鈉樣品表面有利于纖維蛋白原和白蛋白的吸附,但兩者吸附量的差值與表面固定酒石酸系列樣品相比顯著降低。盡管與固定L-酒石酸樣品相比,固定D-酒石酸樣品吸附纖維蛋白原量要多,但是通過(guò)纖維蛋白變性實(shí)驗(yàn)結(jié)果可知,固定D-酒石酸樣品表面上的纖維蛋白原變性程度顯著低于固定L-酒石酸樣品表面的,在隨后的血小板的粘附與激活實(shí)驗(yàn)中,固定D-酒石酸樣品也表現(xiàn)出了更好的抗血小板粘附與激活的能力。為了更進(jìn)一步研究手性對(duì)催化供體釋放一氧化氮能力的影響,本論文用無(wú)手性但具有催化中心的硒代胱胺代替己二胺,硒代胱胺可以催化供體釋放一氧化氮,在材料表面引入催化活性中心,將L-/D-酒石酸接枝到材料表面,在材料的表面引入手性元素。XPS的結(jié)果顯示當(dāng)硒代胱胺接枝到聚多巴胺樣品后,材料表面新出現(xiàn)了 Se元素,通過(guò)材料表面各元素高分辨圖譜的解析可得,接枝上不同手性酒石酸分子樣品的硒元素含量約為0.5%,固定D-酒石酸樣品催化內(nèi)源性供體釋放NO的速率為1.0×10-10mol/cm2,而固定L-酒石酸樣品催化速率為0.7×10-10-10mol/cm2,說(shuō)明固定D-酒石酸樣品具有更優(yōu)的催化內(nèi)源性供體釋放NO的能力。隨后的血小板粘附實(shí)驗(yàn)結(jié)果表明,在血漿中未添加內(nèi)源性一氧化氮供體的情況下,沉積聚多巴胺樣品表面血小板粘附量最多,固定D-酒石酸樣品血小板粘附量少于固定L-酒石酸樣品的,Ti-O表面具有最優(yōu)的抗血小板粘附和激活性能;在血漿中添加供體的情況下,具有催化供體釋放NO的樣品表面血小板粘附數(shù)量明顯減少,固定D-酒石酸樣品表面粘附的血小板數(shù)量最少,顯著低于表面固定L-酒石酸樣品和表面僅固定硒代胱氨樣品。
[Abstract]:Chirality is a basic feature of life system, constitute the organic molecules for life the vast majority of chiral molecules, and showed high chiral, chiral molecules and biological systems in different interactions will exhibit different biological functions. This paper firstly by poly dopamine and hexanediamine system in different construction of chiral Ti-O film the surface is fixed, the chiral tartaric acid system and fixed chiral sodium tartrate system, chiral system for screening, then the chiral surface were screened and fibrinogen denaturation and platelet adhesion and activation of experimental evaluation, study the chiral surface blood compatibility. Two se bond selenocystamine molecule with catalytic ability of endogenous nitric oxide the release of nitric oxide donor, so this paper will also replace selenocystamine 1,6-diaminohexane, in material surface into the catalytic center, finally in the material most Surface grafting chiral tartaric acid, the effect of different chiral tartaric acid fixation on surface selenocystamine and subsequent release of nitric oxide donor catalytic effect on platelet adhesion and activation of.X ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) method for characterization of materials science of modified samples the results show that the surface deposition, polydopamine new N1s peak, and Ti-O on the membrane of Ti2p peak was completely covered, that the polydopamine films were prepared on the surface of the sample. The grafting ratio of N:C increased, because of the introduction of a large number of amino grafting showed that hexanediamine successfully, then grafted chiral tartaric acid and chiral tartaric acid sodium the sample surface increased the proportion of O:C showed that the chiral tartaric acid and chiral sodium tartrate grafted.WCA showed that the surface of the material is fixed with a series of chiral molecules, the water contact angle has little change that The surface does not affect the hydrophobicity of chiral material surface; on plasma proteins such as fibrinogen by QCM-D method for real-time monitoring of each sample, the adsorption of bovine serum albumin, the direct evidence that no significant difference between the number of L- tartaric acid and D- acid tartrate grafted sample surface, but the amount of fibrinogen and bovine serum albumin was then the adsorption of tartaric acid grafted D- sample surface fibrinogen content and bovine serum albumin was significantly more than the grafted L- tartaric acid adsorbed on the surface of the sample, the fixed D- tartaric acid on plasma protein samples showed better affinity, suggesting that the chiral surface has a negligible effect on the protein adsorption behavior for a fixed. L- sodium tartrate samples, results also showed that the immobilized D- sodium tartrate sample surface to fibrinogen and albumin adsorption, but the difference between the adsorption capacity and surface fixed Tartaric acid series samples compared to the significantly reduced. Compared with the fixed L- tartaric acid samples, fixed D- tartaric acid sample adsorption amount to the original fiber protein, but the experimental results showed that fiber protein denaturation, fixed D- tartaric acid samples on the surface of the fibrinogen denaturation degree was significantly lower than that of the fixed L- tartaric acid on the surface of the sample, in then the adhesion and activation of platelets in the experiment, the fixed D- tartaric acid samples also showed better anti platelet adhesion and activation ability. In order to further study on the chiral catalytic ability to influence the release of nitric oxide donor, this paper used selenocystamine achiral but has the catalytic center instead of 1,6-diaminohexane, selenocystamine the donor can catalyze the release of nitric oxide, in the catalytic center into material surface, L-/D- tartaric acid was grafted onto the surface of the material, the introduction of chiral.XPS elements on the surface of the material. The results show that when selenocystamine grafted onto the polydopamine samples, the new material surface appeared Se elements, each element of material surface through high resolution map analysis can be grafted, selenium content of different chiral tartaric acid molecular sample is about 0.5%, fixed rate D- tartaric acid catalyzed endogenous release of NO donor samples for 1 x 10-10mol/cm2, fixed L- tartaric acid sample catalytic rate is 0.7 * 10-10-10mol/cm2, the fixed D- tartaric acid sample has better catalytic ability of endogenous release of NO. The donor platelet adhesion following experimental results show that in the plasma without endogenous nitric oxide donor under the condition of polydopamine deposition surface of platelet adhesion amount most fixed D- tartaric acid samples of platelet adhesion less than fixed L- tartaric acid samples, platelet adhesion and activation of the Ti-O surface has the best performance in plasma; In addition, the number of platelets adhered to the surface of the sample with NO released by the donor decreased significantly. The number of platelets adhered on the fixed D- tartaric acid sample was the least, which was significantly lower than that of the surface fixed L- tartaric acid sample and the surface only fixed selenocysteine sample.

【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O643.36

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 胡珂;劉濤;劉詩(shī)卉;陳俊英;黃楠;;鈦材料表面固定多聚賴(lài)氨酸-肝素納米顆粒以改善血液相容性的研究[J];功能材料;2012年19期

2 鄭細(xì)鳴;黃小軍;徐志康;;聚丙烯微孔膜表面肝素化及其對(duì)低密度脂蛋白的吸附特性[J];高分子學(xué)報(bào);2011年07期

3 翁文;韓景立;陳友遵;黃曉佳;;手性傳感器研究進(jìn)展[J];化學(xué)進(jìn)展;2007年11期

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本文編號(hào)：1668794