本文選題：液液萃取　切入點：氣相色譜氫火焰離子檢測器　出處：《食品科學》2017年24期

【摘要】：采用液液萃取法結(jié)合氣相色譜氫火焰離子檢測器建立一種可同時檢測葡萄酒中13種游離態(tài)單萜烯類物質(zhì)的方法,并采用所建方法檢測了商品菌SC和自選菌KDLYS9-16兩種釀酒酵母發(fā)酵的河北省沙城和昌黎2個產(chǎn)區(qū)的赤霞珠和美樂葡萄酒中的單萜烯類物質(zhì)。結(jié)果顯示,方法的R2為0.996 7~0.999 3,檢出限為0.580~1.073 mg/L,相對標準偏差為0.58%~3.18%,回收率為89.98%~107.34%。4種葡萄酒中的單萜烯類物質(zhì)質(zhì)量濃度都比發(fā)酵前的葡萄汁中高,尤以采用自選高產(chǎn)β-D-葡萄糖苷酶釀酒酵母KDLYS9-16發(fā)酵的昌黎產(chǎn)區(qū)赤霞珠干紅葡萄酒中最高,達到2.855 mg/L,比葡萄汁中增加約3.1倍。赤霞珠葡萄及葡萄酒中單萜烯物質(zhì)質(zhì)量濃度比美樂葡萄高,沙城產(chǎn)區(qū)的2種葡萄汁中單萜烯類物質(zhì)質(zhì)量濃度比昌黎的高,選用不同菌種發(fā)酵的葡萄酒中單萜烯物質(zhì)質(zhì)量濃度和種類不同。綜上,本研究建立方法簡單、靈敏性高、重復性好,可用于葡萄及葡萄酒中單萜烯類物質(zhì)的檢測。
[Abstract]:A method for simultaneous determination of 13 free monoterpenes in wine by liquid-liquid extraction combined with gas chromatographic hydrogen flame ion detector was developed. The monoterpenes in Cabernet Sauvignon and Merlot Wine from Shacheng and Changli regions of Hebei Province were detected by using the established method. The R2 of the method was 0.996 ~ 0.999 3, the detection limit was 0.580 ~ 1.073 mg / L, the relative standard deviation was 0.58 ~ 3.18, and the recovery was 89.98 ~ 107.34%. The concentration of monoterpenes in grape juice was higher than that in grape juice before fermentation. Especially, the Cabernet Sauvignon dry red wine in Changli region, which was fermented by self-selected high-yield 尾 -Dglucosidase Saccharomyces cerevisiae KDLYS9-16, was the highest in Cabernet Sauvignon. The concentration of monoterpene in Cabernet Sauvignon and wine was higher than that in Merlot grape, and the mass concentration of monoterpenes in two kinds of grape juice in Shacheng region was higher than that in Changli. The concentration and species of monoterpenes in wine fermented with different strains were different. In summary, the method was simple, sensitive and reproducible, and could be used for the determination of monoterpenes in grape and wine.
【作者單位】： 河北科技大學生物科學與工程學院;河北省發(fā)酵工程技術(shù)研究中心;
【基金】：河北省自然科學基金項目(C2011208028)
【分類號】：O657;TS262.6

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