本文選題：葫蘆[7]脲　切入點(diǎn)：鹽酸巴馬汀　出處：《山西師范大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：熒光光譜法具有靈敏度高、選擇性好及操作簡(jiǎn)便等特點(diǎn)而被廣泛使用,然而某些有機(jī)藥物分子本身沒(méi)有熒光,所以不能直接通過(guò)熒光光譜法來(lái)測(cè)定。本文借助于第四代超分子主體葫蘆[7]脲(CB[7])與天然異喹啉生物堿巴馬汀(PAL)形成的熒光探針體系,研究了某些有機(jī)藥物分子與熒光探針PAL在CB[7]空腔中的競(jìng)爭(zhēng)反應(yīng)。發(fā)現(xiàn)無(wú)熒光有機(jī)藥物西吡氯銨、苯扎溴銨和鹽酸丁卡因均能使CB[7]-PAL熒光探針體系產(chǎn)生強(qiáng)烈的猝滅作用,而且藥物的濃度與熒光猝滅的速率呈良好的線性關(guān)系,據(jù)此分別建立了測(cè)定西吡氯銨、苯扎溴銨和鹽酸丁卡因熒光光譜法。所建立的方法具有簡(jiǎn)單、快捷、選擇性好、靈敏度高等優(yōu)點(diǎn),并將該方法應(yīng)用到了藥物制劑、血樣及尿樣的檢測(cè),檢測(cè)結(jié)果取得令人滿意的結(jié)果。另外本文采用熒光光譜、紫外光譜、1H NMR等技術(shù)探討了這3種藥物分子與探針在CB[7]空腔中的競(jìng)爭(zhēng)反應(yīng)機(jī)理,可以看出3種藥物分子與CB[7]的包合常數(shù)均大于CB[7]-PAL的包合常數(shù),因此在競(jìng)爭(zhēng)反應(yīng)中探針?lè)肿颖凰幬锓肿訑D出空腔而使探針熒光猝滅。最后通過(guò)密度泛函理論計(jì)算還建立了藥物與超分子相互作用的分子模型。本文可認(rèn)為四個(gè)部分:1、介紹了第4代超分子主體CB[n]的合成、分子結(jié)構(gòu)、分子識(shí)別、分子組裝和分子器件等領(lǐng)域的研究進(jìn)展。簡(jiǎn)述了以CB[n]為主體的熒光增敏法研究和以CB[n]為主體的熒光猝滅法研究進(jìn)展與現(xiàn)狀。2、西吡氯銨(CPC)沒(méi)有熒光,故至今沒(méi)有熒光光譜法測(cè)定CPC的文獻(xiàn)報(bào)道。CB[7]-PAL是一種可以發(fā)射強(qiáng)烈熒光的探針體系,隨著CPC的加入熒光強(qiáng)度明顯降低,且在一定范圍內(nèi)猝滅值與CPC的濃度成正比。通過(guò)一些先進(jìn)技術(shù)探討了其超分子作用機(jī)理,并計(jì)算了CB[7]-CPC超分子包合物的結(jié)合常數(shù),據(jù)此為CPC建立了一種熒光光譜檢測(cè)法。3、通過(guò)熒光光譜法研究了在酸性溶液中苯扎溴銨(BKB)與CB[7]的包合作用,BKB本身沒(méi)有熒光,通過(guò)在CB[7]疏水空腔中與PAL的競(jìng)爭(zhēng)反應(yīng),導(dǎo)致CB[7]-PAL體系熒光猝滅,猝滅值與BKB的濃度呈良好的線性關(guān)系,據(jù)此建立了一種測(cè)定BKB的靈敏度高、選擇性好、重現(xiàn)性好的熒光光譜法。4、采用熒光光譜法,紫外光譜法和1H NMR研究了鹽酸丁卡因(TCH)與CB[7]的相互作用,并通過(guò)密度泛函理論計(jì)算建立了TCH與CB[7]相互作用的分子模型。據(jù)此為T(mén)CH建立了一種以CB[7]為主體、PAL為探針的熒光光譜新方法,結(jié)果表明所建立的方法的靈敏度均高于文獻(xiàn)報(bào)道的TCH光譜分析方法。且該方法可用于測(cè)定藥物制劑和血樣中TCH的含量。
[Abstract]:Fluorescence spectrometry is widely used because of its high sensitivity, good selectivity and simple operation. However, some organic drug molecules do not have fluorescence. Therefore, the fluorescence probe system formed by 4th generation supramolecular host gourd [7] Urea [7] CB [7] and natural isoquinoline alkaloid palmatine palmatine (PAL) was not directly determined by fluorescence spectrometry. The competitive reaction of some organic drug molecules with fluorescent probe PAL in CB [7] cavity was studied. It was found that the fluorescence probe system of CB [7] -pal could be quenched strongly by the non-fluorescent organic drug cepirium chloride, benzalkonium bromide and tetracaine hydrochloride. In addition, the concentration of the drug showed a good linear relationship with the rate of fluorescence quenching. Based on this method, a fluorescence spectrometric method for the determination of cepiramide, benzalkonium bromide and tetracaine hydrochloride was established. The method was simple, rapid and selective. The method has been applied to the detection of pharmaceutical preparations, blood samples and urine samples, and the results are satisfactory. In addition, fluorescence spectra are used in this paper. The competitive reaction mechanism of these three drug molecules and probes in CB [7] cavity was investigated by UV spectra 1H NMR and other techniques. It can be seen that the inclusion constants of the three drug molecules with CB [7] are larger than those of CB [7] -pal. Therefore, in the competitive reaction, the probe molecule is squeezed out of the cavity by the drug molecule to quench the fluorescence of the probe. Finally, the molecular model of the interaction between drug and supramolecular is established by density functional theory. In this paper, four parts can be considered. In this paper, the synthesis of the fourth generation supramolecular host CB [n] is introduced. The research progress in the fields of molecular structure, molecular recognition, molecular assembly and molecular devices. The progress and present situation of fluorescence sensitization method with CB [n] as the main body and fluorescence quenching method with CB [n] as the main body are briefly described. So far, there is no literature report on the determination of CPC by fluorescence spectroscopy. CB [7] -pal is a probe system which can emit strong fluorescence, and the fluorescence intensity decreases obviously with the addition of CPC. The quenching value is proportional to the concentration of CPC in a certain range. The mechanism of supramolecular interaction of CB [7] -CPC is discussed by some advanced techniques, and the binding constants of CB [7] -CPC supramolecular inclusion complexes are calculated. Based on this, a fluorescence spectrometric method for the detection of CPC was established. By means of fluorescence spectroscopy, the competitive reaction with PAL in the hydrophobic cavity of CB [7] was studied by means of fluorescence spectroscopy, which was used to study the inclusion of BKB with CB [7]. The fluorescence quenching of CB [7] -pal system resulted in a good linear relationship between the quenching value and the concentration of BKB. Based on this, a fluorescence spectrometric method with high sensitivity, good selectivity and good reproducibility for the determination of BKB was established. The interaction between tetracaine hydrochloride and CB [7] was studied by UV spectroscopy and 1H NMR. The molecular model of interaction between TCH and CB [7] was established by density functional theory, and a new fluorescence spectrum method with CB [7] as the probe was established for TCH. The results showed that the sensitivity of the established method was higher than that of the TCH spectrometric method reported in the literature, and the method could be used to determine the content of TCH in drug preparations and blood samples.
【學(xué)位授予單位】：山西師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O657.3;R927

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 楊輝;譚業(yè)邦;黃曉玲;王月霞;;葫蘆脲的研究進(jìn)展[J];化學(xué)進(jìn)展;2009年01期

2 劉思敏,吳成泰;葫環(huán)聯(lián)脲新進(jìn)展[J];化學(xué)進(jìn)展;2005年01期

3 劉思敏,吳曉軍,梁峰,姚駿驊,吳成泰;葫[n]環(huán)聯(lián)脲(n=5,6,7,8)與兩種重氮氟硼酸鹽的包合作用研究[J];高等學(xué)校化學(xué)學(xué)報(bào);2004年11期

4 張桂玲,徐周慶,薛賽鳳,祝黔江,陶朱;一類(lèi)新型環(huán)型籠狀化合物——瓜環(huán):(Ⅱ)酸度、IA、IIA金屬離子對(duì)瓜環(huán)溶解性的影響[J];無(wú)機(jī)化學(xué)學(xué)報(bào);2003年06期

5 韓寶航,劉育;葫蘆脲:分子識(shí)別與組裝[J];有機(jī)化學(xué);2003年02期

，

本文編號(hào)：1616413