本文選題：鮮棗　切入點(diǎn)：高效液相色譜-蒸發(fā)光散射檢測(cè)器　出處：《食品科學(xué)》2017年14期 　論文類型：期刊論文

【摘要】：采用高效液相色譜-蒸發(fā)光散射檢測(cè)器建立鮮棗果實(shí)中不同種類可溶性糖含量的測(cè)定方法。結(jié)果表明:適宜分離條件為Waters XBridge~(TM) Amide色譜柱(4.6 mm×250 mm,5μm),流動(dòng)相A為0.2%三乙胺的超純水溶液,流動(dòng)相B為0.2%三乙胺-乙腈溶液,兩相體積比為24∶76,柱溫30℃,霧化管溫度60℃,漂移管溫度60℃,氣流量1.6 L/min,增益值1.0。在該條件下鼠李糖、阿拉伯糖、果糖、甘露糖、葡萄糖、蔗糖、麥芽糖和乳糖均能得到較好分離(分離度≥1.5),在0.099~1.040 ng/μL范圍內(nèi)呈良好線性關(guān)系,8種糖的加標(biāo)回收率在93.1%~111.2%之間,相對(duì)標(biāo)準(zhǔn)偏差均小于5%;檢出限(R_(SN)=3)在0.008~0.03 ng/μL之間。該方法具有操作簡(jiǎn)便、分離效果好、分離時(shí)間短等特點(diǎn),可用于鮮棗果實(shí)中不同種類可溶性糖含量的測(cè)定,與傳統(tǒng)方法相比較更加靈敏準(zhǔn)確,有利于品質(zhì)鑒定。
[Abstract]:A method for the determination of soluble sugars in jujube fruit was established by high performance liquid chromatography (HPLC) and evaporative light scattering detector. The results showed that the optimum separation conditions were as follows: Amide column 4.6mm 脳 250mm ~ 5 渭 m, mobile phase A = 0.2% triethylamine, ultrapure water solution. Mobile phase B is 0.2% triethylamine-acetonitrile solution, the volume ratio of two phases is 24: 76, column temperature is 30 鈩，

本文編號(hào)：1598510