發(fā)布時間：2018-03-11 08:07

  本文選題：pH值　切入點(diǎn)：比色/熒光　出處：《上海師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：基于半菁染料,熒光碳點(diǎn)為熒光母體,設(shè)計、制備了三種高選擇性熒光探針。利用分子內(nèi)電荷轉(zhuǎn)移效應(yīng)(ICT),細(xì)胞內(nèi)吞和靶向基團(tuán)實現(xiàn)了環(huán)境中和和活細(xì)胞內(nèi)微環(huán)境的定性、定量檢測。(1)由于傳統(tǒng)的p H計不能很好地應(yīng)用在微環(huán)境如亞細(xì)胞器和復(fù)雜生物體系中的p H值動態(tài)和精確定量檢測。因此迫切需要研究可監(jiān)測復(fù)雜多變生物體系中p H值變化且具有高靈敏度的p H值響應(yīng)分子探針�；诎胼既玖虾铣闪艘环N比色和熒光雙模態(tài)檢測p H的比率型分子探針CPH。CPH的具有合適的p Ka(6.44),使其可以聚集在溶酶體內(nèi)成為靶向溶酶體的探針。CPH可監(jiān)測經(jīng)抗瘧疾藥氯喹刺激He La細(xì)胞后溶酶體內(nèi)p H值的變化。將CPH固載在試紙上,可實現(xiàn)對環(huán)境中酸性和堿性蒸汽的可逆、動態(tài)、裸眼檢測。這一概念驗證展現(xiàn)出CPH作為檢測溶酶體內(nèi)p H變化的分子工具的潛在應(yīng)用,同時提供了一種經(jīng)濟(jì)、可重復(fù)和快速對酸堿蒸汽的光學(xué)p H探針。(2)溶酶體是酸性囊狀結(jié)構(gòu)的細(xì)胞器,其膜內(nèi)含有大量酶和蛋白質(zhì),p H值是4.5-5.5。因此,發(fā)展通過可視化熒光信號用于溶酶體標(biāo)記和成像對理解與溶酶體功能紊亂相關(guān)的病理學(xué)顯得尤為重要。首次設(shè)計、制備了基于熒光碳點(diǎn)用于標(biāo)記溶酶體的納米探針CDs-PEI-ML。該探針具有良好的水溶性和光穩(wěn)定性。我們推測其成像原理是該納米探針先通過內(nèi)吞進(jìn)入He La細(xì)胞,再通過嗎啉基團(tuán)使其聚集在溶酶體內(nèi)。(3)基于半菁骨架,我們發(fā)展了一種比率型溶酶體探針CPY。該探針具有優(yōu)良的溶酶體靶向性。利用該探針揭示出溶酶體的p H會隨著熱刺激而升高,且這個過程在短時間內(nèi)是不可逆的。
[Abstract]:Three highly selective fluorescent probes were designed and prepared based on hemicyanine dyes with fluorescence carbon spots as the fluorescent parent. The intracellular endocytosis and targeting groups were used to characterize the environmental and living microenvironments by using intramolecular charge transfer effect (ICTI). (1) since the traditional pH meter can not be well applied to dynamic and accurate quantitative detection of pH values in microenvironments such as suborganelles and complex biological systems, there is an urgent need to study and monitor complex and variable biological systems. Middle pH value varied and highly sensitive molecular probe with pH response. Based on hemicyanine dye, a ratio molecular probe CPH.CPH with colorimetric and fluorescence bimodal detection of pH was synthesized, which can be aggregated. The lysosomal probe. CPH can monitor the change of pH value in helium La cells stimulated by chloroquine. The CPH was immobilized on the test paper. This proof of concept demonstrates the potential application of CPH as a molecular tool for detecting changes in pH in lysosomes and provides an economy. The lysosome is an acidic cystosomal organelle containing a large number of enzymes and proteins (pH = 4.5-5.5). The development of visual fluorescent signals for lysosomal labeling and imaging is particularly important in understanding the pathology associated with lysosomal dysfunction. A fluorescent carbon dot based probe CDs-PEI-MLwas prepared for labelling lysosomes. The probe has good water-solubility and photostability. We speculate that the imaging principle of the probe is that the probe first enters He-La cells through endocytosis. Then the morpholine group is used to aggregate it in lysosome. 3) based on hemicyanine skeleton, We have developed a ratio lysosomal probe CPY. the probe has excellent lysosomal targeting. It is revealed that the pH of lysosome increases with heat stimulation and the process is irreversible in a short time.
【學(xué)位授予單位】：上海師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

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本文編號：1597298