本文選題：柴胡疏肝湯　切入點(diǎn)：高效液相色譜　出處：《南方醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：背景與目的柴胡疏肝湯是本課題組根據(jù)癲癇“從肝論治”治療理論組建的復(fù)方,由柴胡、黃芩、白芍等15味中藥組成,具有疏肝、柔肝、養(yǎng)肝、平肝作用,現(xiàn)代研究表明,此方具有降低神經(jīng)興奮性、抗炎和調(diào)節(jié)機(jī)體免疫功能的作用。該方為附屬醫(yī)院中藥房的協(xié)定處方,已在臨床運(yùn)用30余年,在癲癇預(yù)防和治療上取得了確切療效。中藥質(zhì)量的好壞與臨床用藥療效及安全密切相關(guān)。目前柴胡疏肝湯尚未有相關(guān)質(zhì)量控制研究,本文將采用高效液相色譜法對柴胡疏肝湯進(jìn)行系統(tǒng)研究,建立柴胡疏肝湯指紋圖譜,并對其主要成分含量進(jìn)行測定,從整體和部分有效成分上對其質(zhì)量進(jìn)行全面控制,為臨床用藥安全及該方進(jìn)一步研究提供理論依據(jù)。方法1.柴胡疏肝湯高效液相指紋圖譜研究及其主要成分黃芩苷、漢黃芩苷、漢黃芩素、芍藥苷、阿魏酸、甘草苷的含量測定。采用高效液相色譜法,二極陣列檢測器,Agilent ZORBAX SB-C8色譜柱(250 mm×4.6mm,5μm),在檢測波長210nm、柱溫25℃下,進(jìn)樣量為10μL,以乙腈(A)-0.2%磷酸水溶液(B)為流動相進(jìn)行梯度洗脫,檢測時間為65 min。測定10批柴胡疏肝湯色譜圖,評估柴胡疏肝湯整體質(zhì)量的相似度,分析該方共有特征峰藥物歸屬。在相同的色譜條件下,建立其多成分含量測定方法,為柴胡疏肝湯的質(zhì)量控制提供依據(jù)。2.柴胡疏肝湯主要成分柴胡皂苷a、漢黃芩苷、芍藥苷的含量測定。采用高效液相色譜法,二極陣列檢測器,Agilent ZORBAX SB-C8色譜柱(250 mmx4.6mm,5 μm),在檢測波長210nm、柱溫25℃C下,進(jìn)樣量為10μL,以乙腈(A)-0.2%磷酸水溶液(B)為流動相進(jìn)行梯度洗脫,檢測時間為80 min�？疾炝瞬窈碥誥、漢黃芩苷、芍藥苷的穩(wěn)定性、重復(fù)性,測定其含量,為該方質(zhì)量控制提供依據(jù)。結(jié)果1.建立了柴胡疏肝湯高效液相指紋圖譜,10批樣品的相似度較好均大于0.95。確定共有峰有22個,明確各峰所歸屬藥材,其中7個色譜峰可明確化學(xué)成分。同時建立了能同時測定柴胡疏肝湯中主要成分芍藥苷、黃芩苷、漢黃芩苷、漢黃芩素、阿魏酸、甘草苷含量方法。線性關(guān)系良好,平均加樣回收率(n=9)均在 90～105%(RSD3%)。2.建立測定柴胡疏肝湯中主要成分柴胡皂苷a、漢黃芩苷、芍藥苷的含量方法,在24小時內(nèi)穩(wěn)定,重復(fù)性可,線性關(guān)系良好,平均加樣回收率(n=9)均在90～105%(RSD3%)。結(jié)論建立柴胡疏肝湯高效液相指紋圖譜及含量測定方法,該方法重復(fù)性、穩(wěn)定性可,線性關(guān)系、平均加樣回收率良好,可以反映柴胡疏肝湯整體特性。為柴胡疏肝湯質(zhì)量控制及進(jìn)一步研究提供理論基礎(chǔ)。
[Abstract]:Background and objective: ChaiHuShuGan decoction is the research group according to the epilepsy "from the liver" therapy theory established by compound, bupleurum, Scutellaria, Radix Paeoniae Alba composed of 15 herbs, with soothing liver, nourishing liver, liver function, modern research shows that this has reduced nerve excitability, anti-inflammatory and immune regulation function of body. The agreement prescription for Affiliated Hospital Pharmacy in more than 30 years, has been used in clinical, has exact curative effect in the prevention and treatment of epilepsy. Traditional Chinese medicine quality and clinical efficacy and safety are closely related. At present there is no Tangshang Chai Hushu liver related research quality control, this paper will use the high performance liquid chromatography chromatography system research of ChaiHuShuGan Decoction ChaiHuShuGan decoction, establishment of fingerprint, and its main components were determined, to conduct a comprehensive control of its quality from the whole and part of effective components, for clinical use Drug safety and the further research and provide a theoretical basis for the study. Map and its main component baicalin method 1. chaihushugantang HPLC fingerprint, baicalin, wogonin, paeoniflorin, ferulic acid, determination of liquiritin. By HPLC diode array detector, Agilent ZORBAX SB-C8 chromatography column (250 mm * 4.6mm, 5 m), at the detection wavelength of 210nm, the column temperature of 25 DEG C, the injection volume was 10 L (A), acetonitrile -0.2% phosphoric acid solution (B) as the mobile phase with gradient elution. The detection time is 65 min. by the 10 batch of chaihushugantang chromatograms. The similarity of overall quality assessment of ChaiHuShuGan decoction, analysis the common characteristic peaks of drugs belonging. Under the same chromatographic conditions, to establish a method for determination of the content, for the quality control of ChaiHuShuGan Decoction ChaiHuShuGan Decoction.2. provide the basis for the main components of saikosaponin a, baicalin, paeoniflorin The content determination by high performance liquid chromatography diode array detector, Agilent ZORBAX SB-C8 column (250 mmx4.6mm, 5 m), at the detection wavelength of 210nm, the column temperature 25 DEG C, the injection volume was 10 L (A), acetonitrile -0.2% phosphoric acid solution (B) as mobile phase. Gradient elution, detection time is 80 min. The effects of saikosaponin a, baicalin, paeoniflorin, stability, repeatability, determination of its content, provide the basis for the quality control. Results 1. established chaihushugantang HPLC fingerprints of 10 batches of samples of high similarity were more than 0.95. were determined there are 22 clear peaks, each peak belongs to medicine, among which 7 peaks can clear the chemical composition. At the same time to establish a simultaneous determination of paeoniflorin ingredient of ChaiHuShuGan Decoction in glycosides, baicalin, wogonoside, wogonin, ferulic acid, liquiritin. Method of linear relationship, the average sample the recovery rate (n=9 ) were in the range of 90 to 105% (RSD3%).2. determination of ChaiHuShuGan Decoction in main ingredients saikosaponin a, baicalin, paeoniflorin content method, stable in 24 hours, repeatability, good linear relationship, the average recovery rate (n=9) were in the range of 90 to 105% (RSD3%) conclusion. The establishment of chaihushugantang HPLC fingerprint and determination, the method repeatability, stability, linear relationship, the average recovery rate is good, can reflect the overall characteristics of ChaiHuShuGan decoction. It provides the theoretical basis for the quality control of ChaiHuShuGan Decoction and further study.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R286.0;O657.72

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本文編號：1593068