本文選題：雙配體功能化金納米簇　切入點(diǎn)：谷胱甘肽　出處：《青島科技大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：作為新興的熒光探針,雙配體功能化的金納米簇(Au NCs)由于其具有獨(dú)特的光學(xué)物理性質(zhì),良好的生物相容性、尺寸比較小、靈敏性高、選擇性好等一系列優(yōu)點(diǎn),而被廣泛應(yīng)用于生物傳感和生物成像中,由于其潛在的應(yīng)用,蛋白質(zhì)、聚合物、烷烴硫醇以及多肽等材料被作為配體用于熒光金納米簇的合成。由于等離子體增強(qiáng)熒光有著熒光信號放大和有效提高檢測靈敏度的能力,成為一種新興技術(shù),新型的核殼金納米棒結(jié)構(gòu)作為近紅外熒光信號打開的傳感器,可用于高靈敏,高選擇性的檢測。本論文主要研究內(nèi)容如下:(1)在本工作中,我們開發(fā)了一種簡便的一步策略快速制備雙配體功能化的熒光金納米團(tuán)簇(Au NCs),并建立了一種“turn on”方法,用于在水溶液和活體細(xì)胞中快速和選擇性的檢測谷胱甘肽(GSH)。所制備的Au NCs顯示橙色紅色熒光(λem=608 nm),熒光壽命長(5.62μs),斯托克斯位移大(300 nm),穩(wěn)定性比較好,體系的表征主要通過使用熒光光譜表征、高分辨透射電鏡(HRTEM)、動(dòng)態(tài)光散射(DLS)、X射線光電子能譜(XPS)、熒光壽命、紅外光譜(IR)、紫外吸收光譜表征�；贏u NCs和GSH之間與Cu2+的競爭性配位誘導(dǎo)的熒光恢復(fù),目前的“turn on”方法對GSH檢測提供了高靈敏度和優(yōu)異的選擇性,檢測限為9.7 nM。更重要的是,這種“turn on”方法也可以成功應(yīng)用于可視化和監(jiān)測Hep G2細(xì)胞中GSH水平的變化,為診斷應(yīng)用提供潛在的應(yīng)用價(jià)值。(2)光學(xué)可交叉響應(yīng)傳感器陣列最近被公認(rèn)為高通量蛋白質(zhì)檢測和分析的強(qiáng)大技術(shù)。基于六種近紅外熒光雙配體功能化金納米簇(不同種類氨基酸功能化)作為傳感受體,開發(fā)了能夠分析各種蛋白質(zhì)并區(qū)分不同階段乳腺癌患者血清的多維傳感器陣列。這種“化學(xué)鼻子/舌頭”傳感器陣列可同時(shí)有效地區(qū)分十種蛋白質(zhì)。線性判別分析(LDA)的響應(yīng)模式顯示出分析物在低至10 nM的濃度下成功分化,識別精度高。等溫滴定量熱法(ITC)實(shí)驗(yàn)表明,Au NCs主要通過氫鍵和范德華力與蛋白質(zhì)相互作用。此外,通過成功區(qū)分來自乳腺癌患者(早,中,晚)和健康人的血清,證明了該傳感器陣列的最大亮點(diǎn),表明在臨床輔助診斷中的潛力很大。(3)本工作中合成了金納米棒,對金納米棒進(jìn)行了高分辨透射電鏡和紫外吸收光譜表征,實(shí)驗(yàn)結(jié)果說明了合成的金納米棒均勻性比較好,又對金納米棒進(jìn)行了二氧化硅的包覆,高分辨透射電鏡表征顯示了包覆的均勻性。該二氧化硅包覆的金納米棒將有望用于發(fā)展基于等離子體增強(qiáng)熒光的檢測體系。
[Abstract]:As a new fluorescent probe, double ligand functionalized gold nanoclusters (au NCs) have a series of advantages, such as unique optical physical properties, good biocompatibility, small size, high sensitivity, good selectivity and so on. And has been widely used in biosensor and imaging, because of its potential applications, proteins, polymers, Alkyl mercaptan and polypeptides are used as ligands in the synthesis of fluorescent gold nanoclusters. Plasma enhanced fluorescence has become a new technology because of its ability to amplify fluorescence signals and improve detection sensitivity. A new structure of core shell gold nanorods, which is opened by near infrared fluorescence signal, can be used to detect high sensitivity and selectivity. The main contents of this thesis are as follows: 1) in this work, We have developed a simple, one-step strategy for the rapid preparation of double ligand functionalized gold nanoclusters, au NCsN, and a "turn on" method has been established. Au NCs was prepared for rapid and selective detection of glutathione glutathione (GSH) in aqueous solution and in vivo cells. The au NCs showed orange red fluorescence (位 em=608 NmM), long fluorescence lifetime of 5.62 渭 s-1, and a large Stokes shift of 300nmG, and showed good stability. The system was characterized by fluorescence spectroscopy, high resolution transmission electron microscopy (TEM), dynamic light scattering (DLSX) X-ray photoelectron spectroscopy (XPS), and fluorescence lifetime. Based on the competitive coordination between au NCs and GSH and Cu2 induced fluorescence recovery, the current "turn on" method provides high sensitivity and excellent selectivity for GSH detection with a detection limit of 9.7 nm. This "turn on" method can also be successfully used to visualize and monitor the changes of GSH levels in Hep G2 cells. Providing potential application value for diagnostic applications. Optical cross responsive sensor arrays have recently been recognized as a powerful technique for high throughput protein detection and analysis. Based on six near infrared fluorescent double ligand functionalized gold nanoclusters (NIR). Different kinds of amino acid functionalization) as sensing receptors, A multi-dimensional sensor array has been developed that can analyze various proteins and distinguish the serum of breast cancer patients at different stages. This "chemical nose / tongue" sensor array can effectively distinguish ten proteins at the same time. The response patterns of LDAs showed that the analytes differentiated successfully at concentrations as low as 10nM. Isothermal drop quantitative calorimetry (ITC) test showed that au NCs interacted with protein mainly through hydrogen bond and van der Waals force. In addition, by successfully distinguishing serum from breast cancer patients (early, middle, late) and healthy people, The results show that gold nanorods have been synthesized in this work and characterized by high resolution transmission electron microscopy (TEM) and UV absorption spectra. The experimental results show that the synthesized gold nanorods have good uniformity, and the gold nanorods are coated with silica. The high resolution transmission electron microscopy (TEM) shows the uniformity of the coating. The silica coated gold nanorods are expected to be used in the development of plasma-enhanced fluorescence detection systems.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 阮桂色;;電感耦合等離子體原子發(fā)射光譜(ICP-AES)技術(shù)的應(yīng)用進(jìn)展[J];中國無機(jī)分析化學(xué);2011年04期

2 付西軍;;新型樹枝狀化合物的合成與應(yīng)用研究進(jìn)展[J];河南科技學(xué)院學(xué)報(bào)(自然科學(xué)版);2011年06期

3 吳獻(xiàn)花,林洪,李海濤,胡秋芬,楊光宇;用快速分離柱高效液相色譜法測定食品中的重金屬元素的研究[J];食品科學(xué);2005年06期

相關(guān)博士學(xué)位論文 前1條

1 王緒美;熒光納米材料的合成制備、性質(zhì)表征和應(yīng)用研究[D];吉林大學(xué);2012年

，

本文編號：1575037