本文關(guān)鍵詞： 超聲細(xì)胞粉碎提取 響應(yīng)曲面法 六味地黃丸 質(zhì)量控制　出處：《色譜》2017年01期 　論文類型：期刊論文

【摘要】：針對(duì)中藥復(fù)方制劑六味地黃丸的質(zhì)量控制提出了一種多指標(biāo)全藥材整體分析新策略。從組成六味地黃丸的每一味藥材中選取1~2個(gè)藥效成分,即選取熟地黃中的毛蕊花糖苷、山茱萸中的莫諾苷和馬錢苷、牡丹皮中的丹皮酚和芍藥苷、山藥中的尿囊素、茯苓中的茯苓酸、澤瀉中的23-乙酰澤瀉醇B作為六味地黃丸的質(zhì)量控制指標(biāo)成分,采用快速、簡(jiǎn)便、高效的超聲細(xì)胞粉碎提取(UCGE)法對(duì)這8個(gè)指標(biāo)成分進(jìn)行同步提取。用響應(yīng)曲面分析中的Box-Behnken模式對(duì)UCGE提取過程中對(duì)提取效率影響較大的料液比、提取時(shí)間和提取溫度等參數(shù)進(jìn)行了優(yōu)化。得到最優(yōu)的提取條件:料液比1∶45(g/mL)、提取時(shí)間40 min、提取溫度20℃。進(jìn)一步利用超高效液相色譜-二極管陣列檢測(cè)(UPLC-PAD)技術(shù)實(shí)現(xiàn)多指標(biāo)成分在同一根色譜柱上的同步分離和檢測(cè),從而建立六味地黃丸的多指標(biāo)全藥材整體色譜。采用HSS T3色譜柱(50 mm×2.1 mm,1.8μm),以乙腈-水為流動(dòng)相進(jìn)行梯度洗脫,基于指標(biāo)成分的紫外光譜特征選擇5個(gè)檢測(cè)波長同時(shí)檢測(cè),并進(jìn)行了線性關(guān)系、檢出限、定量限、精密度、穩(wěn)定性、重復(fù)性、準(zhǔn)確性等方法學(xué)驗(yàn)證。該研究為六味地黃丸質(zhì)量標(biāo)準(zhǔn)的建立提供了依據(jù),同時(shí)也為中藥復(fù)方制劑提供了一種質(zhì)量控制新模式和新理念。
[Abstract]:According to the quality control of Liuwei Dihuang pills, a new strategy for the whole analysis of multi-index whole medicinal materials was put forward. One or two effective components were selected from each drug of Liuwei Dihuang pills, that is, Ruirin in Rehmannia Rehmanniae was selected. Monoside and marganin in Cornus officinalis, paeonol and paeoniflorin in peony peel, allantoin in yam, Poria cocos in Poria cocos, and 23- acetylalisin B in alisma alisma were used as quality control index components of Liuwei Dihuang pills. The eight index components were extracted synchronously by a simple and efficient ultrasonic cell crushing extraction method. The ratio of material to liquid had a great influence on the extraction efficiency of UCGE by using the Box-Behnken pattern in response surface analysis. The extraction time and temperature were optimized, and the optimal extraction conditions were obtained: the ratio of material to liquid was 1: 45 g / mL, the extraction time was 40 min, and the extraction temperature was 20 鈩，

本文編號(hào)：1502531