本文關(guān)鍵詞： 蛋白質(zhì) 太赫茲 光學(xué)參數(shù) 生理環(huán)境 牛血清蛋白 胃蛋白酶　出處：《吉林大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：蛋白質(zhì)是組成人體一切細(xì)胞、組織的重要有機(jī)大分子,參與幾乎所有重要的生命活動(dòng)。在不同生理環(huán)境下蛋白質(zhì)的定性定量分析是蛋白功能檢查、臨床診斷、康復(fù)檢查以及生物藥品質(zhì)量檢查的重要指標(biāo)。目前生化檢測(cè)方法主要利用蛋白質(zhì)對(duì)應(yīng)抗體的特異性表達(dá)信息獲得相應(yīng)靶分子的種類(lèi)、含量等信息,過(guò)程繁瑣,需要花費(fèi)較多資源。作為一種物理探測(cè)方法,太赫茲(THz)技術(shù)不需要傳統(tǒng)生化檢測(cè)方法中復(fù)雜的標(biāo)記過(guò)程,非常適合對(duì)蛋白質(zhì)直接進(jìn)行無(wú)標(biāo)記的檢測(cè)。由于太赫茲波覆蓋了生物大分子之間/內(nèi)的弱相互作用、骨架振動(dòng)以及偶極子旋轉(zhuǎn)的能級(jí)范圍,通過(guò)生物大分子在太赫茲波段所特有的THz指紋譜可對(duì)生物大分子定性分析。另外蛋白含量及構(gòu)象改變會(huì)對(duì)THz波段光學(xué)參數(shù)產(chǎn)生影響,所以光學(xué)參數(shù)是判定蛋白質(zhì)種類(lèi)、含量以及蛋白質(zhì)變性的主要依據(jù)。本文首先概述了太赫茲波以及太赫茲時(shí)域光譜技術(shù)(THz-TDS)的特性和目前用于生物大分子檢測(cè)的研究進(jìn)展。然后介紹了幾種經(jīng)典透射THz-TDS光學(xué)參數(shù)提取方法。由于超薄微流道的使用法布里-珀羅震蕩變得更加顯著,對(duì)光學(xué)參數(shù)準(zhǔn)確度造成一定的影響。在對(duì)前人算法去粗取精的基礎(chǔ)上,本文開(kāi)發(fā)了更加簡(jiǎn)單且更適用于微納流道的參數(shù)提取算法。最后利用開(kāi)發(fā)算法研究了微納流道中處于不同液態(tài)生理環(huán)境下蛋白質(zhì)的太赫茲光譜特性。主要包括兩方面:不同濃度牛血清蛋白(BSA)的水化作用研究和不同酸堿環(huán)境下胃蛋白酶(Pepsin)構(gòu)象變化研究。實(shí)驗(yàn)結(jié)果表明,光學(xué)參數(shù)(如折射率和吸收系數(shù))與BSA溶液濃度不符合朗博-比爾定律,因?yàn)殡S著B(niǎo)SA溶液濃度的增加BSA蛋白分子含量增高導(dǎo)致其周?chē)乃瘜酉嗷ブ丿B。Pepsin溶液的吸收系數(shù)在pH=3和pH=5,pH=7以及pH=8.5,pH=11分別處于三種狀態(tài),因?yàn)镻epsin在pH=2~4的生理環(huán)境下才能保證正常的空間構(gòu)象,在pH=5~7環(huán)境中結(jié)構(gòu)發(fā)生了一些變化,當(dāng)pH8.5則發(fā)生不可逆的變性。上述研究成果為T(mén)Hz光譜技術(shù)定性定量檢測(cè)蛋白質(zhì)分子提供了可靠的依據(jù)。
[Abstract]:Proteins are important organic molecules that make up all the cells and tissues of the human body, and participate in almost all important life activities. In different physiological environments, the qualitative and quantitative analysis of proteins is the examination of protein function and clinical diagnosis. At present, biochemical methods mainly use the specific expression information of protein corresponding antibody to obtain the information of the target molecule, such as the type and content of the corresponding target molecule, and the process is tedious. It takes a lot of resources. As a physical method of detection, terahertz (THz) technology does not require a complex labeling process in traditional biochemical methods. The THz wave covers the energy levels of the weak / internal interactions between / within biomolecules, the skeleton vibration, and the dipole rotation. Biological macromolecules can be qualitatively analyzed by THz fingerprinting of biological macromolecules in the terahertz band. In addition, protein content and conformation change will affect the optical parameters of THz band, so the optical parameters are to determine the type of proteins. In this paper, the properties of terahertz wave and THz TDSs, and the research progress of THz TDSs used in the detection of biomolecules are reviewed. Then several classical transmissions are introduced. THz-TDS optical parameters extraction method. Because of the use of ultra-thin microchannel Fabry-Perot oscillations become more prominent, The accuracy of optical parameters is affected to some extent. In this paper, a more simple and suitable parameter extraction algorithm is developed. Finally, the terahertz spectral characteristics of proteins in micronanorods under different liquid physiological conditions are studied by using the developed algorithm. Aspects: the hydration of bovine serum protein (BSA) with different concentrations and the conformation changes of pepsin in different acid-base environments. The optical parameters (such as refractive index and absorption coefficient) do not conform to Lambert-Beer 's law with the concentration of BSA solution. With the increase of the concentration of BSA solution, the content of BSA protein molecule increased, and the absorption coefficient of the surrounding hydration layer overlapped. The absorption coefficient of BSA solution was in three states: pH=3, pH ~ (5 +) ~ (5), pH ~ (7) and pH ~ (8.5) ~ (+) ~ (-1) ~ (-1), respectively. Because Pepsin can guarantee normal spatial conformation under the physiological environment of pH=2~4, some changes have taken place in the structure of pH=5~7. When pH8.5 is irreversibly denatured, the above results provide a reliable basis for the qualitative and quantitative detection of protein molecules by THz spectroscopy.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：Q6-33;O657.3

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