本文關(guān)鍵詞： 多金屬氧酸鹽 過(guò)氧化氫 顯色免疫檢測(cè) 肌氨酸 三聚氰胺　出處：《東北師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：顯色免疫檢測(cè)是通過(guò)體系顏色的變化有針對(duì)性的檢測(cè)某種物質(zhì),在分析中通常會(huì)用到生物酶,比如辣根過(guò)氧化物酶(HRP),但其容易受到外界環(huán)境的影響而變質(zhì)。多金屬氧酸鹽(Polyoxometalates,POMs)是一類具有氧化還原性的金屬-氧簇化合物,具有類似納米的結(jié)構(gòu),其催化氧化性能可以應(yīng)用于很多方面,包括顯色免疫檢測(cè)等。本論文基于POMs可以活化H_2O_2的性質(zhì),擬設(shè)計(jì)H_2O_2的HRP模擬化合物,并代替HRP。前列腺癌嚴(yán)重困擾著男性患者,在初期檢測(cè)中經(jīng)常用到的方法有:直腸指檢、直腸超聲、前列腺穿刺活檢、電子計(jì)算機(jī)斷層掃描(CT)和多重內(nèi)反射(MIR)等,這些檢測(cè)手段不僅會(huì)對(duì)患者造成極大的心理負(fù)擔(dān)以及生理傷害,還會(huì)導(dǎo)致對(duì)病情的誤判或過(guò)度治療,所以發(fā)展一種高效便捷、具有人文關(guān)懷的方法迫在眉睫。無(wú)良商家在奶粉中添加三聚氰胺的事件,讓社會(huì)對(duì)孩子食用的奶制品的質(zhì)量存在質(zhì)疑。因此,我們希望設(shè)計(jì)一種方便快捷、簡(jiǎn)單有效的方法來(lái)檢測(cè)三聚氰胺,使食品檢測(cè)更加切實(shí)可行,使食品安全得到保障。因此,我們合成一系列不同金屬取代的雜多酸鹽,研究其通過(guò)活化H_2O_2氧化有機(jī)底物TMB(C_(16)H_(20)N_2)的性能,探究其催化活性與氧化性之間的關(guān)系,并由此研究通過(guò)其性能檢測(cè)肌氨酸和三聚氰胺的方法。1、制備不同金屬取代的硅鎢雜多酸鹽:[SiW_(11)O_(39)M(H_2O)]~(6-)、[SiW_(10)O_(36)M_2(H_2O)_2]~(4-)、[SiW_9O_(37)M)3(H_2O)_3]~(8-),(M=Cu~(2+),Co~(2+),Mn~(2+),Fe~(2+));稀土金屬取代的[SiW_(11)O_(37)L(H_2O)]n-(L=La~(3+),Ce~(4+));并利用紅外光譜(IR)、紫外光譜(UV)、循環(huán)伏安(CV)等方法對(duì)其結(jié)構(gòu)進(jìn)行表征,并比較其氧化還原性能;研究其催化檢測(cè)肌氨酸的活性,探究其線性關(guān)系,建立基于SiW_9Mn3快速檢測(cè)肌氨酸的方法,檢測(cè)線性范圍為2.40×10-2~7.86×10~(-4) mol/L,最低檢測(cè)限為4.96×10-3mol/L。2、制備不同金屬取代的磷鎢雜多酸鹽:[PW_(11)O_(39)M]~(5-),[PW_9O_(34)M_3]~(3-)(M=Cu~(2+),Co~(2+),Mn~(2+),Fe~(2+));并利用IR、UV、CV等方法對(duì)其結(jié)構(gòu)進(jìn)行表征,并比較其氧化還原性能;研究其催化檢測(cè)三聚氰胺活性,探究其線性關(guān)系,建立基于PW_(11)Co快速檢測(cè)三聚氰胺的方法,檢測(cè)線性范圍為1.0×10~(-4)-1.5×10-6mol/L,最低檢測(cè)限為2.5×10-6mol/L。
[Abstract]:Colour immunoassay is the specific detection of a substance by changing the color of the system, and bioenzymes are commonly used in the analysis. Horseradish peroxidase (HRP), for example, is susceptible to deterioration due to the external environment. Polyoxometales polyoxometalates (POMs) are a kind of redox metal-oxygen cluster compounds with similar nanostructures. The catalytic oxidation performance can be used in many fields, including color immunoassay. Based on the property that POMs can activate Hs _ 2O _ 2, this paper intends to design a HRP mimic compound of H _ 2O _ 2 and replace HRP.Prostate cancer seriously affects male patients. The methods often used in early detection include rectal digital examination, rectal ultrasound, prostate biopsy, computed tomography (CT) and multiple internal reflex (mir), etc. These testing methods will not only cause great psychological burden and physiological harm to patients, but also lead to misjudgment or overtreatment of the condition, so develop an efficient and convenient method. There is an urgent need for humane care. The incident of adding melamine to milk powder by unscrupulous merchants has raised questions about the quality of dairy products consumed by children. Therefore, we hope to design a convenient and fast one. A simple and effective method to detect melamine, make food detection more feasible and ensure food safety. Therefore, we synthesize a series of heteropolyacids substituted by different metals. To study its performance by activating the organic substrates of H _ 2O _ 2 oxidation, TMB-C _ s _ s _ 16 / T _ T _ I _ _ _ 2) and to explore the relationship between its catalytic activity and its oxidizing properties, And through the method of detecting creatine and melamine, we have studied the preparation of silicotungsten heteropoly salt substituted by different metals: [SiWA11ON39Mh _ 2O)] 6-C, [SiW_(10)O_(36)M_2(H_2O)_2] 6-C, [SiW_9O_(37)M)3(H_2O)_3] 8-MCuCu2MnCoC _ (2) M _ (2) MCuC _ (2) MN _ (2) O _ (2) H _ (2) O _ (2); rare earth metal-substituted [Siw _ (11) O _ (11) O _ (11) O _ (H _ 2O))] n-L Lahuo _ (3) (n ~ (1)) -L ~ (+) ~ (1) (n) -L ~ ((1)) -lLaA-1 / C ~ (3) C ~ (+ +) ~ (-) ~ (3) ~ (-) ~ (-) ~ (-) ~ (?). The structure was characterized by ACV and other methods. Its redox performance was compared, the activity of its catalytic detection of sarcosine was studied, the linear relationship was explored, and a method for rapid detection of sarcosine based on SiW_9Mn3 was established. The linear range of detection is 2.40 脳 10 ~ (-2) ~ 7.86 脳 10 ~ (-4) mol / L, and the lowest detection limit is 4.96 脳 10 ~ (-3) mol 路L ~ (-2). Different metal-substituted phosphotungstophosphorus heteropolyates: [PW_(11)O_(39)M] -5 ~ (-), [PW_9O_(34)M_3] ~ (3) ~ (-) ~ ((1)) ~ (2) CoCo22 ~ (2) mn ~ (2) mn ~ (2) C ~ (2) C ~ (2) C ~ (2) C ~ (2) is used to characterize its structure, and to compare its redox properties, and to study its catalytic activity for the detection of melamine, and to study its catalytic activity. The linear relationship was investigated and a rapid detection method for melamine based on PW_(11)Co was established. The linear range was 1.0 脳 10 ~ (-4) -1.5 脳 10 ~ (-6) mol / L, and the minimum detection limit was 2.5 脳 10 ~ (-6) mol / L.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O652.3

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本文編號(hào)：1497853