本文關(guān)鍵詞：基于鈾酰配合物共振光散射法測(cè)定1,6-二磷酸果糖和鈾　出處：《南華大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 1 6-二磷酸果糖 鈾 共振光散射 salophen 納米金 雙極配體

【摘要】：1,6-二磷酸果糖(F-1,6-BP)和鈾對(duì)人體健康和環(huán)境有著重要的影響,因此建立快速、靈敏度高、選擇性好的檢測(cè)F-1,6-BP和鈾的新方法具有十分重要的意義。為此,本文合成了羧基salophen和磷酸乙醇-酰胺基-salophen(PAS),利用這兩種配體分別建立了基于鈾酰配合物修飾的金納米粒子測(cè)定F-1,6-BP的共振光散射(RLS)分析法和基于鈾酰與PAS組裝形成超分子聚合物測(cè)定鈾的RLS分析法,為F-1,6-BP和鈾的檢測(cè)提供了新的手段。本文第一章緒論部分首先介紹了1,6二磷酸果糖(F-1,6-BP)和鈾的現(xiàn)有檢測(cè)方法的特點(diǎn),接著介紹了Salophen基本性質(zhì)和應(yīng)用研究、納米金的性質(zhì)和應(yīng)用以及RLS技術(shù)的基本原理和應(yīng)用。本文第二章中我們制備了一種修飾金納米粒子,鈾酰-salophen-半胱胺-金納米粒子(U-Sal-Cy-GNPs),并以此為探針建立了一種具有高靈敏度的RLS法檢測(cè)溶液中F-1,6-BP的含量,該方法無(wú)須經(jīng)過(guò)分離可直接檢測(cè)。其中,U-Sal-Cy-GNPs的制備按照以下幾個(gè)步驟進(jìn)行,首先將半胱胺結(jié)合在納米金的表面形成半胱胺包絡(luò)的納米金(Cy-GNPs),接著將Cy-GNPs與羧基salophen通過(guò)酰胺反應(yīng)形成Sal-Cy-GNPs,最后鈾酰離子與Sal-Cy-GNPs上的四齒配體發(fā)生配位反應(yīng),由于鈾酰-salophen能特異性結(jié)合磷酸基團(tuán),一分子的FBP分子很容易連接兩個(gè)U-Sal-Cy-GNPs,從而引起GNPs聚集,導(dǎo)致強(qiáng)烈的RLS信號(hào)產(chǎn)生,根據(jù)體系RLS的變化來(lái)檢測(cè)F-1,6-BP的量,并對(duì)實(shí)驗(yàn)的各項(xiàng)影響因素進(jìn)行了優(yōu)化。結(jié)果表明,我們的方法測(cè)定的F-1,6-BP濃度線性范圍為2.5~75 nmol/L,檢出限為0.91 nmol/L。該方法已成功應(yīng)用到實(shí)際樣品的檢測(cè),回收率在96.3%~102.5%之間。本文第三章我們合成了一種既含有四齒配位基又含有單齒配位基的雙極配體,磷酸乙醇基-酰胺基-salophen(PAS),其四齒配位基和單齒配位基分別為salophen單元和磷酸基團(tuán),該雙極配體能與鈾(Ⅵ)或鈾酰離子配位自組裝形成金屬-超分子聚合物,導(dǎo)致體系的RLS信號(hào)顯著增強(qiáng),由此建立了RLS法檢測(cè)痕量鈾的新方法。在最適宜的條件下,我們的方法有較高的靈敏度和良好的選擇性,測(cè)得鈾(Ⅵ)檢出限為0.24 ng/m L。該方法已成功用于實(shí)際樣品的檢測(cè),回收率在97.1%-102.6%之間。
[Abstract]:Fructose-6-diphosphate (FDP) and uranium have important effects on human health and environment. Therefore, a rapid, sensitive and selective detection of F-1 has been established. The new methods of 6-BP and uranium are of great significance. Therefore, carboxyl salophen and ethanol-amide-phosphate salophenone were synthesized. The two ligands were used to establish the gold nanoparticles modified with uranyl complexes for the determination of F-1. The resonance light scattering (RLS) analysis of 6-BP and the RLS analysis of uranium based on the assembly of uranyl and PAS into supramolecular polymers are F-1. In the first chapter of this paper, the author introduces the characteristics of the existing methods for the detection of uranium and fructose 1h6 diphosphate (F-1O6-BPP) as well as the characteristics of the existing methods. Then the basic properties and applications of Salophen, the properties and applications of gold nanoparticles, and the basic principles and applications of RLS technology are introduced. In the second chapter, we prepare a modified gold nanoparticles. Uranyl-salophena-cysteamine gold nanoparticles (U-Sal-Cy-GNPs) were used as probes to establish a highly sensitive RLS method for the determination of F-1 in solution. The content of 6-BP can be detected directly without separation. The preparation of U-Sal-Cy-GNPs is carried out according to the following steps. First, cysteamine was bound to the surface of gold nanoparticles to form cysteamine envelop (Cy-GNPs). Cy-GNPs was then reacted with carboxyl salophen to form Sal-Cy-GNPs via amides. Finally, uranyl ions reacted with tetradentate ligands on Sal-Cy-GNPs because uranyl-salophen could specifically bind phosphate groups. One molecule of FBP molecules can easily connect two U-Sal-Cy-GNPs, thus causing the aggregation of GNPs and the production of strong RLS signals. According to the change of system RLS, the amount of F-1N 6-BP was detected, and the influencing factors of the experiment were optimized. The results showed that the F-1 was determined by our method. The linear range of 6-BP concentration is 2.5 ~ 75 nmol / L and the detection limit is 0.91 nmol / L. the method has been successfully applied to the detection of real samples. The recovery was between 96.3% and 102.5%. In Chapter 3, we synthesized a bipolar ligand containing both tetradentate and monodentate coordination groups. The four and monodentate coordination groups were salophen unit and phosphate group, respectively. The bipolar ligand was able to self-assemble with uranium (鈪，

本文編號(hào)：1435231