本文關(guān)鍵詞：氨基酸及衍生物對(duì)金屬離子識(shí)別及藥物分子作用的研究　出處：《合肥工業(yè)大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 氨基酸 藥物小分子 熒光光譜 相互作用

【摘要】：熒光分析法具有分析快速、高效、用樣少和檢測(cè)限低等優(yōu)點(diǎn),廣泛應(yīng)用于近代化學(xué)分析等領(lǐng)域。芳香族氨基酸中的色氨酸(Trp),酪氨酸(Tyr),苯丙氨酸(Phe)本身具有熒光特性,其在熒光檢測(cè)及藥物分析等領(lǐng)域具有很廣闊的應(yīng)用價(jià)值。本文應(yīng)用三種芳香族氨基酸(Trp、Tyr和Phe)借助熒光分析法,進(jìn)行了以下工作：以L-苯丙氨酸為原料,研究了三種不同體系對(duì)L-苯丙氨酸甲酯鹽酸鹽合成的影響。在最優(yōu)反應(yīng)條件下,成功合成三種氨基酸甲酯鹽酸鹽。以7-羥基香豆素為原料經(jīng)過(guò)兩步合成得到7-(乙酸氧基)香豆素,其與L-苯丙氨酸甲酯通過(guò)酰胺化反應(yīng)合成了一種新型香豆素衍生物N-乙�；�-(氧-7-香豆素)-3-苯基-2-氨基-丙酸甲酯,其結(jié)構(gòu)經(jīng)FT-IR和1HNMR表征。并進(jìn)行了最終產(chǎn)物在離子檢測(cè)方面的研究,最終表明：N-乙酰基-(氧-7-香豆素)-3-苯基-2-氨基-丙酸甲酯對(duì)二價(jià)鉛離子具有和好的識(shí)別能力,其在離子檢測(cè)和識(shí)別等方面具有潛在的應(yīng)用價(jià)值。借助于熒光光譜分析法,在模擬人體pH生理?xiàng)l件下,分別在兩種不同的溫度條件下(25℃和37℃)研究了藥物小分子(氫化可的松、制菌霉素)與色氨酸、酪氨酸以及苯丙氨酸之間的相互作用,其中主要研究了其相互之間的相互作用類(lèi)型、結(jié)合位點(diǎn)數(shù)、結(jié)合常數(shù)以及相互作用力類(lèi)型。結(jié)果表明：氨基酸(色氨酸、酪氨酸、苯丙氨酸)由于藥物小分子(氫化可的松、制菌霉素)的加入均產(chǎn)生明顯的熒光猝滅效應(yīng);結(jié)合位點(diǎn)均為1：1；其中HYD-Trp和HYD-Tyr作用主要是氫鍵或范德華力,HYD-Phe作用主要是疏水作用,在NYS-Trp和NYS-Tyr的作用力主要是氫鍵或范德華力。,NYS-Phe的作用力主要是疏水作用。
[Abstract]:Fluorescence analysis has the advantages of fast analysis, high efficiency, less sample use and low detection limit. It has been widely used in modern chemical analysis and other fields, such as tryptophan in aromatic amino acids, tyrosine tyrosine tyrosine Tyr. etc. Phenylalanine (PHE) has its own fluorescence characteristics and has a wide application value in the field of fluorescence detection and drug analysis. In this paper, three kinds of aromatic amino acids TRP have been applied. Tyr and PHE) were used for the following work: L- phenylalanine was used as raw material. The effects of three different systems on the synthesis of L-phenylalanine methyl ester hydrochloride were studied. Three kinds of amino acid methyl ester hydrochloride were synthesized successfully and 7-( oxy) coumarin was synthesized from 7-hydroxycoumarin by two steps. A novel coumarin derivative, N-acetyl- (ox-7-coumarin) -3-phenyl-2-amino-propionate, was synthesized by amidation of L-phenylalanine methyl ester. Its structure was characterized by FT-IR and 1H NMR, and the final product was studied in ion detection. In the end, it is shown that the methyl ester 1: N- acetyl- (ox-7-coumarin) -3-phenyl-2-amino-propionate has a good ability to recognize divalent lead ions. It has potential application value in ion detection and recognition. The interaction of small molecules (hydrocortisone, mycomycin) with tryptophan, tyrosine and phenylalanine was studied at 25 鈩，

本文編號(hào)：1425021