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  本文關(guān)鍵詞：碳量子點(diǎn)的制備及其在熒光傳感器中的應(yīng)用　出處：《湖南大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 碳量子點(diǎn) 熒光傳感器 有機(jī)磷殺蟲劑 谷胱甘肽

【摘要】：熒光分析法不僅操作簡單,而且響應(yīng)時間快、靈敏度高、選擇性好、在實(shí)時檢測等方面均具有突出的優(yōu)勢,已被廣泛應(yīng)用于生物檢測領(lǐng)域。熒光碳量子點(diǎn)是碳納米材料家族的一種新型成員,近幾年熒光碳量子點(diǎn)在各物質(zhì)的生化分析檢測方面得到了廣泛的關(guān)注和應(yīng)用。碳量子點(diǎn)不僅僅有優(yōu)良的光學(xué)性能與小尺寸特性,而且碳量子點(diǎn)的毒性低生物相容性好,成為生物成像的最佳選擇。因此,本研究通過制備熒光性能好的熒光碳量子點(diǎn),構(gòu)建了兩種基于碳量子點(diǎn)的熒光傳感器,分別實(shí)現(xiàn)了對氧磷、谷胱甘肽的選擇性熒光分析檢測。研究的主要內(nèi)容概括如下：(1)碳量子點(diǎn)的制備方案多種多樣,探索一種耗時短、合成設(shè)備簡單的制備方法,對于碳量子點(diǎn)的研究與發(fā)展有重要的意義。第二章中采用微波加熱乙二胺-檸檬酸溶液制備了氮摻雜的碳量子點(diǎn)(NC-dots),以組氨酸-檸檬酸作前驅(qū)體,采用水熱合成法制備了碳量子點(diǎn)(C-dots)。利用動態(tài)光散射、紅外光譜、紫外光譜、熒光光譜等多種方法對碳量子點(diǎn)的形貌與結(jié)構(gòu)進(jìn)行表征�？疾炝颂剂孔狱c(diǎn)的光穩(wěn)定性與熒光量子產(chǎn)率,并研究了環(huán)境pH改變對碳量子點(diǎn)熒光強(qiáng)度的影響。(2)有機(jī)磷殺蟲劑在人類生產(chǎn)中廣泛應(yīng)用的同時也帶來了環(huán)境污染和食品安全問題,建立簡單、易操作、高靈敏的有機(jī)磷殺蟲劑檢測方法有重要意義。第三章中,我們構(gòu)建了一種基于NC-dots的熒光猝滅型傳感器檢測有機(jī)磷殺蟲劑。NC-dots被吸附在納米金顆粒(AuNPs)表面組裝成“NC-dots殼”,NC-dots與AuNPs之間發(fā)生熒光共振能量轉(zhuǎn)移(FRET),NC-dots的熒光被猝滅。乙酰膽堿酯酶(AchE)能催化底物碘化硫代乙酰膽堿(ATI)水解生成硫代膽堿,能取代AuNPs表面的NC-dots,使NC-dots解吸附隨之熒光恢復(fù)。有機(jī)磷殺蟲劑存在時,AChE的催化活性被抑制,NC-dots熒光恢復(fù)程度被抑制。有機(jī)磷殺蟲劑的濃度可以通過NC-dots的熒光強(qiáng)度來定量。此傳感器用于分析檢測對氧磷,其靈敏度高,選擇性好,對氧磷的濃度在1.0×10-4-1.0×10-9 g/L范圍內(nèi),NC-dots的熒光與對氧磷的濃度之間出現(xiàn)了兩個線性響應(yīng)區(qū)間,最低檢測值為1.0×10-9 g/L。該傳感器能用于實(shí)際樣品的檢測,提供了一種快速,靈敏監(jiān)測食品中有機(jī)磷含量的方法。(3)谷胱甘肽(GSH)是人體內(nèi)重要的抗氧化劑和自由基清除劑,在抗衰老、抗輻射、清除自由基、解毒、保護(hù)肝臟和抗癌等方面有重要作用。第四章中,根據(jù)銅離子(Cu2+)能與C-dots生成C-dots/Cu2+的復(fù)合物猝滅C-dots的熒光,GSH與Cu2+有更強(qiáng)的結(jié)合能力,重新使C-dots的熒光被恢復(fù),制備了熒光增強(qiáng)型傳感器檢測GSH。Cu2+猝滅C-dots的過程在0-80μM符合Stern-Volmer方程。經(jīng)過條件優(yōu)化,在最佳實(shí)驗(yàn)條件下,GSH的濃度在20-150μM的范圍內(nèi)與C-dots的熒光強(qiáng)度成線性關(guān)系。與現(xiàn)有檢測方法相比,該方法操作簡單,分析速度快,對GSH的檢測具有特異性。
[Abstract]:The fluorescence analysis method is not only simple and fast response time, high sensitivity, good selectivity, has outstanding advantages in real-time detection, has been widely used in the field of biological detection. Fluorescent carbon quantum dots is a new member of the family of carbon nano materials, in recent years the fluorescent carbon dots has received attention and widely used in biochemical analysis and determination of each substance. CQDS not only has optical properties and excellent characteristics of small size, and low toxicity of carbon dots with good biocompatibility, become the best choice for bio imaging. Therefore, the study of fluorescent carbon quantum dots fluorescence properties through the preparation, building two a carbon fluorescence sensor based on quantum dots, were achieved by paraoxon, selective fluorescence analysis of glutathione detection. The main research contents are as follows: (1) a variety of schemes for preparation of carbon quantum dots on diversity. A cable short time, preparation method of synthesis of simple equipment, has an important significance for the research and development of carbon quantum dots. The use of microwave heating ethylenediamine citric acid solution for the second chapter carbon quantum dots doped by nitrogen (NC-dots), with histidine - citric acid as precursor, carbon quantum dots. Prepared by hydrothermal synthesis (C-dots). By using dynamic light scattering, infrared spectroscopy, UV spectroscopy, morphology and structure of fluorescence spectroscopy and other methods of carbon quantum dots were characterized. The influences of the light stability and fluorescence quantum yield of carbon quantum dots, and study the environmental impact of pH changes on the fluorescence intensity of carbon quantum dots at the same time. (2) organophosphorus insecticides are widely used in the human production has also brought environmental pollution and food safety issues, the establishment of a simple, easy to operate, has important significance of organophosphorus pesticide detection methods with high sensitivity. In the third chapter, we construct A NC-dots fluorescence quenching type sensor for the detection of organophosphorus pesticides based on.NC-dots adsorbed on gold nanoparticles (AuNPs) surface mount "NC-dots shell", fluorescence resonance energy transfer between NC-dots and AuNPs (FRET), the fluorescence of NC-dots was quenched. Acetylcholine esterase (AchE) can catalyze the substrate acetylthiocholine iodide (ATI) hydrolysis of thiocholine, can replace AuNPs NC-dots on the surface of the NC-dots desorption with fluorescence recovery. The presence of organophosphorus pesticides, the catalytic activity of AChE was inhibited by inhibition of NC-dots fluorescence recovery degree. The concentration of organophosphorus pesticides by fluorescence intensity of NC-dots. This sensor is used for quantitative detection and analysis of phosphorus oxychloride. The high sensitivity, good selectivity, paraoxon concentration of 1 * 10-4-1.0 * 10-9 in the range of g/L, the two linear response region between NC-dots fluorescence and paraoxon concentration Between the minimum detection value of 1 * 10-9 g/L. of the sensor can be used for the detection of actual samples, provides a fast and sensitive monitoring method of organic phosphorus content in food. (3) glutathione (GSH) is an important body of antioxidant and free radical scavenger, anti radiation, anti-aging, free radical scavenging. Detoxification, has an important role in protecting the liver cancer and so on. In the fourth chapter, according to the copper (Cu2+) complexes with C-dots fluorescence quenching of C-dots generated C-dots/Cu2+, GSH and Cu2+ have stronger binding ability, to make the fluorescence of C-dots was restored, the preparation process of the fluorescence enhancement type sensor for the detection of GSH.Cu2+ by C-dots in line with the Stern-Volmer equation in 0-80 M. After optimization, under the optimum conditions, the concentration of GSH fluorescence intensity in the range of 20-150 and C-dots M in a linear relationship. Compared with the existing detection method, this method operation is simple. Single, fast analysis, the detection of GSH is specific.

【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：O657.3
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本文編號：1356736