鴨跖草在不同土壤中對銅污染的響應(yīng)
發(fā)布時間：2019-05-08 19:15
【摘要】：本研究主要解決以下三個問題：普通鴨跖草(即非礦區(qū)采集,且未經(jīng)馴化的鴨跖草)對土壤銅耐受和富集能力；鴨跖草對銅污染環(huán)境的響應(yīng)機(jī)理；普通鴨跖草修復(fù)銅污染土壤的可行性。本研究中進(jìn)行了兩種不同土壤加銅的盆栽鴨跖草試驗(yàn),觀察了鴨跖草在不同土壤中的生長狀況,分析了鴨跖草養(yǎng)分含量和銅含量,并分析兩種不同土壤的銅形態(tài),結(jié)果表明,土壤性質(zhì)和銅水平顯著影響鴨跖草的生長、生理指標(biāo)、養(yǎng)分含量和銅含量,鴨跖草生長和土壤加銅也影響土壤銅的形態(tài)與土壤理化性質(zhì),酸性土壤中銅的脅迫更強(qiáng)。通過盆栽實(shí)驗(yàn)和化學(xué)分析可知：(1)潮土中普通鴨跖草對土壤銅的耐受和富集能力在加銅濃度低于200 mg/kg時較好,鴨跖草銅富集量在50 mg/kg時達(dá)到最大值,當(dāng)加銅濃度較高時,銅脅迫作用較為明顯,鴨跖草的銅耐受和富集能力減弱；紅壤中普通鴨跖草對土壤銅的耐受和富集能力非常有限,當(dāng)加銅濃度高于50 mg/kg時,植株逐步衰弱直至死亡,但這并不能否定其在低濃度銅污染土壤中的銅耐受和富集力。(2)鴨跖草通過提高體內(nèi)酶活性、葉綠素含量等方法抵抗重金屬毒性,或吸收更多的養(yǎng)分以使自身更加茂盛,從而提高植株對銅污染環(huán)境的耐性。(3)隨著加銅濃度的提高,弱酸可提取態(tài)銅含量與有效銅含量的變化趨勢基本一致,說明土壤弱酸可提取態(tài)銅對有效銅含量具有重要意義,當(dāng)加銅濃度為50 mg/kg時土壤中弱酸可提取態(tài)銅分配系數(shù)最低,為19.40%,證明普通(非礦區(qū)采集且未經(jīng)馴化)鴨跖草在銅污染程度不太嚴(yán)重的情況下,不但可以吸收土壤中的銅來達(dá)到生物修復(fù)效果,還可以通過將土壤中的銅轉(zhuǎn)化為不易被植物吸收的形態(tài)來達(dá)到減輕土壤污染,保護(hù)土壤生態(tài)環(huán)境,降低土壤中銅對作物的危害的目的。綜合本研究的一系列結(jié)果可知,在潮土中,普通鴨跖草修復(fù)銅污染土壤的可行性較高,至少500 mg/kg加銅濃度下可以存活并吸收和轉(zhuǎn)化土壤中的有效銅,但在紅壤中,普通鴨跖草修復(fù)銅污染土壤的效果欠佳,植株難以生存,對治理紅壤中較高濃度的銅污染意義不大。
[Abstract]:In this study, the following three problems were solved: the tolerance and enrichment of copper in soil, the response mechanism of common herbage to copper pollution, and the non-acclimated and non-acclimated ones, which was not collected in mining area, and the mechanism of response to copper pollution was also discussed in this study. Feasibility of remediation of copper-contaminated soil by common alpinia vulgaris. In this study, the pot experiment of two different soils with copper addition was carried out to observe the growth status, nutrient content and copper content in different soils, and to analyze the copper speciation in two different soils, and the results showed that: (1) the growth status of Dactyceps canadensis in different soil was observed, and the content of copper in two different soils was also analyzed. Soil properties and copper level significantly affected the growth, physiological index, nutrient content and copper content, and the growth and soil copper addition also affected the form and physical and chemical properties of soil copper. The stress of copper in acid soil was stronger than that in acid soil. Through pot experiment and chemical analysis, it was found that: (1) the tolerance and enrichment ability of common plantlets to soil copper was better when the concentration of copper was less than 200 mg/kg, and the maximum copper concentration was reached when the concentration of copper was 50 mg/kg. When the concentration of copper was higher, the effect of copper stress was more obvious, and the ability of tolerance and enrichment of copper was weakened. The tolerance and enrichment of copper in red soil was very limited. When the concentration of copper was higher than 50 mg/kg, the plant gradually weakened and died. But this does not negate its copper tolerance and enrichment in low-concentration copper-contaminated soils. (2) Duck metatarsal resists heavy metal toxicity by increasing enzyme activity and chlorophyll content in the body, or absorbs more nutrients to make itself more prosperous. (3) with the increase of copper concentration, the change trend of weak acid extractable copper content and available copper content was basically the same as that of available copper content. The results show that the extractable copper in soil is of great significance to the available copper content. When the concentration of copper is 50 mg/kg, the partition coefficient of weak acid extractable copper in soil is the lowest (19.40%). It is proved that the common (non-mining area collected and unacclimated) metatarsus can not only absorb copper from the soil to achieve the bioremediation effect when the copper pollution is not too serious. The purpose of reducing soil pollution, protecting soil ecological environment and reducing the harm of copper in soil to crops can also be achieved by transforming the copper in soil into a form which is not easily absorbed by plants. According to a series of results obtained in this study, it was found that it was feasible to repair copper-contaminated soil in tidal soil. At least 500 mg/kg of copper concentration could survive, absorb and transform available copper in soil, but in red soil, it was found that it was feasible to restore copper-contaminated soil at least 500 mg/kg, but in red soil, the available copper could be absorbed and transformed. It is difficult for plants to survive in the remediation of copper contaminated soil by common plantlets, which is of little significance to the treatment of copper pollution in red soil at a higher concentration.
【學(xué)位授予單位】：湖北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X53
本文編號：2472165
[Abstract]:In this study, the following three problems were solved: the tolerance and enrichment of copper in soil, the response mechanism of common herbage to copper pollution, and the non-acclimated and non-acclimated ones, which was not collected in mining area, and the mechanism of response to copper pollution was also discussed in this study. Feasibility of remediation of copper-contaminated soil by common alpinia vulgaris. In this study, the pot experiment of two different soils with copper addition was carried out to observe the growth status, nutrient content and copper content in different soils, and to analyze the copper speciation in two different soils, and the results showed that: (1) the growth status of Dactyceps canadensis in different soil was observed, and the content of copper in two different soils was also analyzed. Soil properties and copper level significantly affected the growth, physiological index, nutrient content and copper content, and the growth and soil copper addition also affected the form and physical and chemical properties of soil copper. The stress of copper in acid soil was stronger than that in acid soil. Through pot experiment and chemical analysis, it was found that: (1) the tolerance and enrichment ability of common plantlets to soil copper was better when the concentration of copper was less than 200 mg/kg, and the maximum copper concentration was reached when the concentration of copper was 50 mg/kg. When the concentration of copper was higher, the effect of copper stress was more obvious, and the ability of tolerance and enrichment of copper was weakened. The tolerance and enrichment of copper in red soil was very limited. When the concentration of copper was higher than 50 mg/kg, the plant gradually weakened and died. But this does not negate its copper tolerance and enrichment in low-concentration copper-contaminated soils. (2) Duck metatarsal resists heavy metal toxicity by increasing enzyme activity and chlorophyll content in the body, or absorbs more nutrients to make itself more prosperous. (3) with the increase of copper concentration, the change trend of weak acid extractable copper content and available copper content was basically the same as that of available copper content. The results show that the extractable copper in soil is of great significance to the available copper content. When the concentration of copper is 50 mg/kg, the partition coefficient of weak acid extractable copper in soil is the lowest (19.40%). It is proved that the common (non-mining area collected and unacclimated) metatarsus can not only absorb copper from the soil to achieve the bioremediation effect when the copper pollution is not too serious. The purpose of reducing soil pollution, protecting soil ecological environment and reducing the harm of copper in soil to crops can also be achieved by transforming the copper in soil into a form which is not easily absorbed by plants. According to a series of results obtained in this study, it was found that it was feasible to repair copper-contaminated soil in tidal soil. At least 500 mg/kg of copper concentration could survive, absorb and transform available copper in soil, but in red soil, it was found that it was feasible to restore copper-contaminated soil at least 500 mg/kg, but in red soil, the available copper could be absorbed and transformed. It is difficult for plants to survive in the remediation of copper contaminated soil by common plantlets, which is of little significance to the treatment of copper pollution in red soil at a higher concentration.
【學(xué)位授予單位】：湖北大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X53
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