一株苯酚降解菌HY-1的篩
發(fā)布時(shí)間：2018-11-23 09:36
【摘要】：本研究采用富集培養(yǎng)的方法,從淮安市清江石化廠的廢水污泥中分離得到一株能夠高效降解苯酚的菌株,命名為HY-1。對菌株HY-1進(jìn)行了生理特性研究,采用PCR方法擴(kuò)增其16S r RNA基因序列,并進(jìn)行測定。結(jié)果表明,菌株HY-1可以在以苯酚為唯一碳源的無機(jī)培養(yǎng)基上生長,對苯酚具有較強(qiáng)的降解能力。該菌株的16S r DNA序列與不動(dòng)桿菌Acinetobacter sp.的序列相似度均在97%以上,與Acinetobacter beijerinckii(Accession FN433036)的親緣關(guān)系最近,結(jié)合形態(tài)特征、生理特性以及系統(tǒng)發(fā)育分析,將其初步鑒定為Acinetobacter sp.HY-1�？疾炝司闔Y-1生長及降解苯酚的基本特性,測定不同的苯酚初始質(zhì)量濃度、溫度、p H值、裝液量及外加鹽濃度對該菌生長及降解苯酚能力的影響。結(jié)果表明,在35°C,p H為7.5,裝液量為50m L,接種量為20%,搖床振蕩速度120r/min的條件下,培養(yǎng)6h后可使500mg/L的苯酚降解率達(dá)到95%以上,高濃度的外加鹽對該菌生長和降酚能力均有較大抑制作用,固定化微生物技術(shù)提高了苯酚降解率。采用濕法加工對凹土進(jìn)行提純,再進(jìn)行熱改性、酸改性和有機(jī)改性制備改性凹土,通過對改性凹土的表征,研究結(jié)構(gòu)和性質(zhì)變化。實(shí)驗(yàn)結(jié)果表明:經(jīng)過酸改性與有機(jī)改性的凹土比表面積更大,有機(jī)改性凹土的親水性更強(qiáng)、表面電荷分布更多。采用凹土吸附法與生物降解法相結(jié)合,利用改性凹土載體對菌株進(jìn)行固定化,并對其降解含酚廢水的效果進(jìn)行研究。研究表明,采用吸附法將菌株固定于改性凹土載體對苯酚進(jìn)行降解,降解性能最好的載體是有機(jī)改性凹土-菌種復(fù)合載體,其苯酚降解率達(dá)97%,酸改性凹土-菌種復(fù)合載體次之,其降解率達(dá)84%,而最差的是原凹土-菌種復(fù)合載體,降解率僅為61%。
[Abstract]:In this study, a high efficient phenol degrading strain was isolated from wastewater sludge of Qingjiang Petrochemical Plant in Huaian City by means of enrichment and culture, and named as HY-1.. The physiological characteristics of the strain HY-1 were studied. The 16s r RNA gene sequence was amplified by PCR, and the 16s r RNA gene was sequenced. The results showed that the strain HY-1 could grow on inorganic medium with phenol as the sole carbon source and had strong degradation ability to phenol. 16s r DNA sequence and Acinetobacter sp. of Acinetobacter spp. The sequence similarity of Acinetobacter beijerinckii (Accession FN433036 was over 97%, and its relationship with Acinetobacter beijerinckii (Accession FN433036 was the most recent. Combined with morphological characteristics, physiological characteristics and phylogenetic analysis, it was preliminarily identified as Acinetobacter sp.HY-1.. The basic characteristics of the growth and degradation of phenol by HY-1 were investigated. The effects of different initial concentration of phenol, p H value of temperature, the amount of solution and the concentration of added salt on the growth and degradation of phenol were determined. The results showed that the phenol degradation rate of 500mg/L could reach more than 95% under the conditions of 35 擄C ~ (-1) pH = 7.5, 50 mL liquid loading, 20 inoculum and 20 shaking speed 120r/min. After 6 hours of culture, the phenol degradation rate of 500mg/L was more than 95%. High concentration of external salt inhibited the growth and the ability of reducing phenol, and the immobilized microorganism technology improved the degradation rate of phenol. The modified attapulgite was prepared by wet processing, thermal modification, acid modification and organic modification. The change of structure and properties of the modified attapulgite was studied through the characterization of the modified attapulgite. The experimental results show that the surface area of acid-modified and organic modified attapulgite is larger, the hydrophilicity and surface charge distribution of organically modified attapulgite are stronger. A modified attapulite carrier was used to immobilize the strain and its effect on the degradation of phenolic wastewater was studied by combining the adsorption of attapulite with the biodegradation method. The results showed that the best carrier for degradation of phenol was organic modified attapulgite, and the degradation rate of phenol was 97%. The degradation rate of acid-modified attapulgite-strain compound carrier was 84%, while the worst was the original attapula-strain compound carrier, the degradation rate was only 61%.
【學(xué)位授予單位】：西南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X703;X172
本文編號(hào)：2351101
[Abstract]:In this study, a high efficient phenol degrading strain was isolated from wastewater sludge of Qingjiang Petrochemical Plant in Huaian City by means of enrichment and culture, and named as HY-1.. The physiological characteristics of the strain HY-1 were studied. The 16s r RNA gene sequence was amplified by PCR, and the 16s r RNA gene was sequenced. The results showed that the strain HY-1 could grow on inorganic medium with phenol as the sole carbon source and had strong degradation ability to phenol. 16s r DNA sequence and Acinetobacter sp. of Acinetobacter spp. The sequence similarity of Acinetobacter beijerinckii (Accession FN433036 was over 97%, and its relationship with Acinetobacter beijerinckii (Accession FN433036 was the most recent. Combined with morphological characteristics, physiological characteristics and phylogenetic analysis, it was preliminarily identified as Acinetobacter sp.HY-1.. The basic characteristics of the growth and degradation of phenol by HY-1 were investigated. The effects of different initial concentration of phenol, p H value of temperature, the amount of solution and the concentration of added salt on the growth and degradation of phenol were determined. The results showed that the phenol degradation rate of 500mg/L could reach more than 95% under the conditions of 35 擄C ~ (-1) pH = 7.5, 50 mL liquid loading, 20 inoculum and 20 shaking speed 120r/min. After 6 hours of culture, the phenol degradation rate of 500mg/L was more than 95%. High concentration of external salt inhibited the growth and the ability of reducing phenol, and the immobilized microorganism technology improved the degradation rate of phenol. The modified attapulgite was prepared by wet processing, thermal modification, acid modification and organic modification. The change of structure and properties of the modified attapulgite was studied through the characterization of the modified attapulgite. The experimental results show that the surface area of acid-modified and organic modified attapulgite is larger, the hydrophilicity and surface charge distribution of organically modified attapulgite are stronger. A modified attapulite carrier was used to immobilize the strain and its effect on the degradation of phenolic wastewater was studied by combining the adsorption of attapulite with the biodegradation method. The results showed that the best carrier for degradation of phenol was organic modified attapulgite, and the degradation rate of phenol was 97%. The degradation rate of acid-modified attapulgite-strain compound carrier was 84%, while the worst was the original attapula-strain compound carrier, the degradation rate was only 61%.
【學(xué)位授予單位】：西南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X703;X172
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