【摘要】：為了從海綿共附生微生物中尋找天然高效抗污損活性物質(zhì),本文針對前期研究獲取的4株具有顯著抗硅藻附著的活性菌株進行活性驗證和比較,選出其中最優(yōu)菌株進行菌種鑒定及發(fā)酵條件優(yōu)化.比較了活性菌株對小新月菱形藻(Nitzschia closterium)、咖啡雙眉藻(Amphora coffeaeformis)、碎片菱形藻(Nitzschia frustulum)附著的抑制活性,確定菌株UST050418-715為最優(yōu)菌株;通過16S r DNA的測序分析,結(jié)合平板的菌落形態(tài)和掃描電鏡下菌體特征,鑒定活性菌株UST050418-715為短小芽孢桿菌(Bacillus pumilus).為優(yōu)化該菌株的培養(yǎng)條件,選擇了酵母粉、蛋白胨、Na Cl、p H值進行四因素三水平的正交實驗,對菌株培養(yǎng)條件進行優(yōu)化.發(fā)現(xiàn)選擇的4個因素對應(yīng)的菌株發(fā)酵產(chǎn)物對硅藻附著的抑制活性大小不同,影響顯著性順序是酵母粉蛋白胨Na Clp H值,綜合菌株發(fā)酵產(chǎn)物活性、粗提物產(chǎn)量和菌株生長情況進行分析,確定菌株UST050418-715基于海洋2216E培養(yǎng)基的最佳發(fā)酵條件為:蛋白胨含量7.5 g/dm3,酵母粉含量3 g/dm3,Na Cl含量10.45 g/dm3,p H值9.5.
[Abstract]:In order to search for natural high effective anti-fouling active substances from spongy co-epiphytic microbes, the activity of four active strains with significant anti-diatom adhesion was tested and compared in this paper. The optimal strains were selected to identify the strains and optimize the fermentation conditions. The inhibitory activity of the active strain on (Nitzschia frustulum) attachment of (Amphora coffeaeformis), fragments of (Nitzschia closterium), was compared. The optimal strain was UST050418-715. By sequencing the 16s r DNA, combining the colony morphology of the plate and the characteristics of the bacteria under scanning electron microscope, the active strain UST050418-715 was identified as Bacillus pumilus (Bacillus pumilus). In order to optimize the culture conditions of the strain, orthogonal experiments of four factors and three levels were carried out with yeast powder, peptone and Na Cl,p H to optimize the culture conditions of the strain. It was found that the inhibitory activity of the fermentation products of the four selected factors on the attachment of diatoms was different, and the significant order of influence was the Na Clp H value of yeast peptone, which combined the activity of the fermentation products of the strains. The yield of crude extract and strain growth were analyzed. The optimum fermentation conditions of strain UST050418-715 were determined as follows: peptone 7.5 g / dm3, yeast powder 3 g / dm3Na Cl 10.45 g / dm3. P H value 9.5.
【作者單位】： 揚州大學(xué)環(huán)境科學(xué)與工程學(xué)院;揚州大學(xué)海洋科學(xué)與技術(shù)研究所;
【基金】：國家自然科學(xué)基金資助項目(41306131,41106113,41271521) 教育部科學(xué)技術(shù)研究重點項目資助項目(211065) 江蘇省自然科學(xué)基金資助項目(BK2012267,BK20130440) 江蘇省教育廳高校自然科學(xué)基金資助項目(13KJB180029,11KJB170011)
【分類號】：X55
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本文編號：2317460