【摘要】：以一種農作物秸稈(簡稱MG)為載體,采用高效石油烴降解菌群制備固定化微生物,在花盆中模擬石油污染土壤的原位修復。在污染土壤中分別加入秸稈(MG)、游離菌、秸稈(MG)+游離菌、秸稈(MG)固定化微生物,并以只含土著菌的土壤樣品為對照,定期測定不同修復方式下土樣中石油烴、腐殖質、胡敏酸含量和微生物數量,考察微生物對石油污染土壤的修復作用及MG腐解對修復的影響。結果表明,隨著修復的進行,MG在土壤中逐漸腐解,土壤中腐殖質和胡敏酸質量分數明顯增高,加入固定化微生物的土樣中腐殖質和胡敏酸質量分數增長率最高,分別增加了44.58%和39.27%;加入固定化微生物的土樣修復35d的石油烴降解率最高,達到41.78%,且微生物數量最多,其次為添加MG+游離菌的土樣,石油烴降解率為31.75%,均高于只含游離菌土樣的石油烴降解率27.83%。載體MG腐解產生的腐殖質和胡敏酸對石油污染土壤的修復起到了明顯的促進作用。
[Abstract]:The immobilized microorganism was prepared by high efficient petroleum hydrocarbon degrading microflora using a crop straw (MG) as carrier, and in situ remediation of oil-contaminated soil was simulated in flowerpot. The immobilized microorganisms of straw (MG), free bacteria, straw (MG) free bacteria and straw (MG) were added to the polluted soil, and the petroleum hydrocarbon and humus in soil samples under different remediation methods were determined periodically with the control of soil samples containing only indigenous bacteria. The content of Hu Min acid and the quantity of microorganism were studied to investigate the remediation of petroleum contaminated soil by microorganism and the effect of MG decomposition on remediation. The results showed that MG decomposed gradually in soil with remediation, humus and Hu Min acid content in soil increased obviously, humus and Hu Min acid mass fraction in soil samples added immobilized microorganism increased the highest. The degradation rate of petroleum hydrocarbon was the highest (41.78%) in the soil sample with immobilized microorganism for 35 days, and the number of microorganism was the highest, followed by the soil sample supplemented with MG free bacteria, which increased by 44.58% and 39.27% respectively, and the degradation rate of petroleum hydrocarbon was the highest in 35 days after the immobilized microorganism was added. The degradation rate of petroleum hydrocarbon was 31.75, which was higher than that of soil sample containing only free bacteria 27.83%. Humus produced by carrier MG decomposition and Hu Min acid played a significant role in the remediation of petroleum contaminated soil.
【作者單位】： 中國石油大學化學工程學院環(huán)境與安全工程系;
【基金】：山東省自然科學基金項目(ZR2014BM023) 中國石油科技創(chuàng)新基金項目(2009D-5006-07-01) 青島市科技發(fā)展指導計劃項目(KJZD-12-65-jch) 中央高校基本科研業(yè)務費專項資金(11CX05011A) 中國石油大學(華東)研究生創(chuàng)新工程(CX-1219)、(CX2013035) 研究生金點子項目(JDZ1405030) 中央高�；究蒲袠I(yè)務費專項資金(14CX06101A)資助
【分類號】：X172;X53

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