發(fā)布時間：2018-09-10 10:44

【摘要】：本試驗通過在好氧顆粒污泥生長前期投加混凝劑的方式,考察混凝劑的投加對顆粒生長的影響,比較在顆粒生長過程中污泥外觀形態(tài)及其基本特性的區(qū)別,測定整個過程中污泥的細胞表面相對疏水性及Zeta電位,并用三維熒光光譜法分析胞外多聚物(EPS)在顆粒生長過程中的變化規(guī)律及其與污泥特性的相關(guān)性。研究結(jié)果如下:(1)投加混凝劑PAC的反應(yīng)器里培養(yǎng)的好氧污泥顆粒生成時間和完全顆�；瘯r間分別比未投加的反應(yīng)器提前3d和12d,其強度和比重也分別比對照組高出2.05%和0.032,混凝強化造粒條件下形成的顆粒污泥外形規(guī)則、結(jié)構(gòu)密實,且沉降絮凝性能均高于對照組。(2)反應(yīng)器R1中的顆粒污泥完全顆粒化后的細胞表面疏水性維持在75%左右,Zeta電位也穩(wěn)定在-11m V左右,高于沒有投加PAC的反應(yīng)器中的污泥細胞表面疏水性和Zeta電位,但污泥的表面疏水性和Zeta電位的變化都表現(xiàn)出相同的趨勢,且可以明顯看出R1的Zeta電位值的上升速率比未投加PAC的R2迅速。(3)對混凝強化造粒條件下顆粒污泥的各形態(tài)EPS三維熒光光譜分析發(fā)現(xiàn),生長期松散型胞外多聚物(LB-EPS)的峰點為溶解性微生物代謝產(chǎn)物,及少量的芳香蛋白類物質(zhì)和腐殖酸類物質(zhì),顆粒成熟后溶解性微生物代謝產(chǎn)物和蛋白類物質(zhì)的熒光強度均減小,腐殖酸類物質(zhì)消失,而溶解型胞外多聚物(S-EPS)和緊密型胞外多聚物(TB-EPS)的峰點及強度在兩階段無明顯變化。(4)分析好氧顆粒污泥形成過程中EPS的含量變化發(fā)現(xiàn),S-EPS含量和TB-EPS含量在整個過程中無明顯規(guī)律,而LB-EPS含量隨顆粒生長逐漸升高,顆粒成熟后逐漸降低至穩(wěn)定,其中的主要成分蛋白質(zhì)含量和LB-EPS含量變化趨勢基本相同,而多糖含量基本保持在1-5 mg/g MLSS。(5)LB-EPS與S-EPS和TB-EPS相比,與好氧污泥顆粒化的關(guān)系有非常密切的關(guān)系。LB-EPS與好氧顆粒污泥的SVI有較強的線性相關(guān)性,其相關(guān)系數(shù)為:r=0.812。而LB-EPS中的PN含量與好氧污泥相對疏水性及其Zeta電位也有一定的相關(guān)性,其相關(guān)系數(shù)分別為:r=-0.996和r=-0.937。
[Abstract]:In this experiment, the effect of coagulant on the growth of aerobic granular sludge was investigated by adding coagulant in the early stage of the growth of aerobic granular sludge, and the difference of the appearance and basic characteristics of sludge in the process of granular growth was compared. The relative hydrophobicity and Zeta potential of the cell surface of the sludge were measured during the whole process. The variation of the extracellular polymer (EPS) in the process of granular growth and its correlation with the characteristics of the sludge were analyzed by three dimensional fluorescence spectrometry. The results are as follows: (1) the granulation time and the complete granulation time of aerobic sludge cultured in the reactor with coagulant PAC were 3 days and 12 days earlier than that of the reactor without coagulant respectively, and the intensity and specific gravity of aerobic sludge in the reactor were 2.05% higher than that in the control group, respectively. And 0.032, the shape rules of granular sludge formed under coagulation-enhanced granulation, (2) the hydrophobicity of granular sludge in reactor R1 was maintained at about 75% and Zeta potential was about -11 MV. The surface hydrophobicity and Zeta potential of sludge were higher than those of the reactor without PAC, but the changes of surface hydrophobicity and Zeta potential of sludge showed the same trend. And it can be seen that the rise rate of Zeta potential of R1 is faster than that of R2 without adding PAC. (3) Three-dimensional EPS fluorescence spectrum analysis of different forms of granular sludge under coagulation-enhanced granulation is found. The peak point of loose extracellular polymer (LB-EPS) in growing stage was soluble microbial metabolites, a small amount of aromatic protein and humic acids, and the fluorescence intensity of metabolites and proteins of dissolved microbes decreased after particle maturation. Humic acids disappear, However, the peak point and intensity of dissolved extracellular polymer (S-EPS) and compact extracellular polymer (TB-EPS) did not change significantly in the two stages. (4) the changes of EPS content during aerobic granular sludge formation showed that the contents of S-EPS and TB-EPS were not regular in the whole process. However, the content of LB-EPS increased with the increase of particle growth, and decreased to a stable level after maturation. The content of protein and LB-EPS in the main components were the same, while the content of polysaccharide was maintained at 1-5 mg/g MLSS. (5) LB-EPS, compared with that of S-EPS and TB-EPS. LB-EPS has a strong linear correlation with the SVI of aerobic granular sludge, and its correlation coefficient is: 1 / r ~ (0.812). The relative hydrophobicity and Zeta potential of aerobic sludge were also correlated with the content of PN in LB-EPS, and the correlation coefficients were: 1 r-0.996 and r-0.937, respectively.
【學(xué)位授予單位】：西安建筑科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X703

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