撲草凈降解菌DY-1的篩
發(fā)布時間：2018-08-26 21:43
【摘要】：撲草凈(Prometryn),又稱撲蔓盡、割草佳,1959年由瑞士嘉基(Geigy)公司創(chuàng)制。化學(xué)名稱為2-甲硫基-4,6二(異丙胺基)-1,3,5-三氮苯,屬于均三氮苯類選擇性除草劑。撲草凈是均三嗪類除草劑最具有代表性的農(nóng)藥之一。撲草凈結(jié)構(gòu)穩(wěn)定,一般條件下不會發(fā)生水解、光解以及自然降解,殘效時間較長,在自然界中能夠持久存在。隨著撲草凈的大量使用以及使用范圍的擴(kuò)大,撲草凈會隨降雨或地表水淋沖或植物根系的滲透作用,遷移至地下水,并在水體中富集,通過食物鏈的富集進(jìn)一步轉(zhuǎn)移給動植物、水體生物并帶來危害,再通過動物遷徙和捕食繼續(xù)遷移轉(zhuǎn)化,最終影響人類的健康。本實驗采用富集培養(yǎng)法,從綏化市北林區(qū)長期施用撲草凈的水稻田中篩選出一株能夠高效降解撲草凈的菌株,命名為DY-1。該菌株能夠以撲草凈作為唯一碳源生長,通過高效液相色譜檢測DY-1可在48 h內(nèi)將培養(yǎng)基中濃度為50 mg/L的撲草凈降解,降解率為99.1%,降解效果較為顯著。菌株在生長48 h后,通過改變某一因素的數(shù)值(溫度、pH或菌液初始濃度)并且保證另外兩個因素不變,對其進(jìn)行OD600值的測定,確定降解菌DY-1的最適生長條件:溫度為30℃,pH為7.0,菌液初始濃度為接種量的3%-5%。其最適碳源為蔗糖,最適氮源為硝酸銨。通過對降解菌DY-1的形態(tài)觀察,生理生化特征以及16S r DNA序列同源性結(jié)果分析,鑒定該菌株為假單胞菌(Pseudomonas sp.),將降解菌DY-1的16S rDNA基因序列在Genbank中注冊,登錄號為KU054384。該研究為土壤中撲草凈的生物降解以及受撲草凈污染土壤的生物修復(fù)提供了理論依據(jù)。
[Abstract]:Puccinia (Prometryn), also known as fluttering, mowing grass, set up in 1959 by (Geigy), Switzerland. The chemical name is 2-methylthio -4N 6 bis (isopropylamino) -1N 3N 3-triazobenzene, which belongs to the selective herbicide of m-triazobenzene. Propofol is one of the most representative pesticides of triazine herbicides. The structure of Puccinia mongolicum is stable, and the hydrolysis, photolysis and natural degradation will not take place under general conditions. The residual effect is longer, and it can exist in nature for a long time. With the extensive use and the expansion of its scope of use, Puccinomium can migrate to groundwater with rainfall or surface water leaching or the infiltration of plant roots, and is enriched in water bodies and further transferred to plants and animals through enrichment in the food chain, Water organisms bring harm, and then through the migration of animals and predators continue to migrate and transform, and ultimately affect the health of human beings. By using enrichment culture method, a strain which can degrade Pucalyx DY-1. was selected from the paddy field in the north forest area of Suihua city for a long time. The strain was able to grow with propofol as the sole carbon source. DY-1 could be degraded in culture medium with concentration of 50 mg/L within 48 h by HPLC. The degradation rate was 99.1, and the degradation effect was remarkable. After 48 hours of growth, the OD600 value of the strain was determined by changing the value of one factor (temperature pH or initial concentration of the liquid) and ensuring that the other two factors remained unchanged. The optimum growth conditions were determined as follows: the temperature was 30 鈩，
本文編號：2206246
[Abstract]:Puccinia (Prometryn), also known as fluttering, mowing grass, set up in 1959 by (Geigy), Switzerland. The chemical name is 2-methylthio -4N 6 bis (isopropylamino) -1N 3N 3-triazobenzene, which belongs to the selective herbicide of m-triazobenzene. Propofol is one of the most representative pesticides of triazine herbicides. The structure of Puccinia mongolicum is stable, and the hydrolysis, photolysis and natural degradation will not take place under general conditions. The residual effect is longer, and it can exist in nature for a long time. With the extensive use and the expansion of its scope of use, Puccinomium can migrate to groundwater with rainfall or surface water leaching or the infiltration of plant roots, and is enriched in water bodies and further transferred to plants and animals through enrichment in the food chain, Water organisms bring harm, and then through the migration of animals and predators continue to migrate and transform, and ultimately affect the health of human beings. By using enrichment culture method, a strain which can degrade Pucalyx DY-1. was selected from the paddy field in the north forest area of Suihua city for a long time. The strain was able to grow with propofol as the sole carbon source. DY-1 could be degraded in culture medium with concentration of 50 mg/L within 48 h by HPLC. The degradation rate was 99.1, and the degradation effect was remarkable. After 48 hours of growth, the OD600 value of the strain was determined by changing the value of one factor (temperature pH or initial concentration of the liquid) and ensuring that the other two factors remained unchanged. The optimum growth conditions were determined as follows: the temperature was 30 鈩，
本文編號：2206246
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