本文選題：枯草芽孢桿菌 + 銅綠微囊藻　； 參考：《中國環(huán)境科學(xué)》2015年06期

【摘要】：為探討枯草芽孢桿菌(Bacillus subtilis)對(duì)銅綠微囊藻(Microcystis aeruginosa)的抑制效果,在實(shí)驗(yàn)室條件下,研究了枯草芽孢桿菌不同生長時(shí)期(延遲期、對(duì)數(shù)期、穩(wěn)定期和衰亡期)無菌濾液對(duì)銅綠微囊藻生長的影響、枯草芽孢桿菌抑制銅綠微囊藻生長的作用方式以及無菌濾液影響下銅綠微囊藻丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和光合色素含量的變化.結(jié)果顯示:枯草芽孢桿菌對(duì)數(shù)期、穩(wěn)定期和衰亡期濾液抑藻效果明顯好于延遲期,作用第8d,對(duì)銅綠微囊藻的去除率分別達(dá)到81.19%、91.41%、91.82%;4個(gè)處理組銅綠微囊藻的葉綠素a含量均顯著低于對(duì)照組.添加穩(wěn)定期濾液后,銅綠微囊藻MDA含量顯著升高,SOD活性先升高后降低;在對(duì)光合色素的影響中,類胡蘿卜素受到的影響不如葉綠素a顯著.結(jié)果表明,枯草芽孢桿菌對(duì)銅綠微囊藻的抑制效果是通過分泌胞外物質(zhì)實(shí)現(xiàn)的,且分泌物具有很強(qiáng)的熱穩(wěn)定性.推測(cè)該胞外分泌物能夠破壞光合色素,影響光合作用,抑制藻細(xì)胞的生長;同時(shí)抑制SOD活性,使細(xì)胞膜脂過氧化程度不斷加深,進(jìn)而破壞藻細(xì)胞的完整性,表現(xiàn)出對(duì)藻很強(qiáng)的抑制效果.
[Abstract]:In order to study the inhibitory effect of Bacillus subtilis on microcystis aeruginosa, the growth stages of Bacillus subtilis were studied in laboratory. The effects of aseptic filtrate on the growth of microcystis aeruginosa, the way of Bacillus subtilis inhibiting the growth of microcystis aeruginosa and the malondialdehyde (MDA) content of microcystis aeruginosa under the influence of aseptic filtrate. Changes of superoxide dismutase (SOD) activity and photosynthetic pigment content. The results showed that the algal inhibition effect of filtrate in logarithmic, stable and decaying stage of Bacillus subtilis was better than that in delayed stage. On the 8th day, the removal rates of microcystis aeruginosa reached 81.191.41 and 91.82, respectively, and the chlorophyll a content in the four treatment groups was significantly lower than that in the control group. The MDA content of microcystis aeruginosa increased at first and then decreased after adding stable filtrate, and the effect of carotenoid on photosynthetic pigments was less significant than that on chlorophyll a. The results showed that the inhibition effect of Bacillus subtilis on microcystis aeruginosa was achieved by secreting extracellular substances and the secretion had strong thermal stability. It is speculated that the extracellular secretion can destroy photosynthetic pigment, affect photosynthesis, inhibit the growth of algal cells, inhibit SOD activity, make the degree of lipid peroxidation of cell membrane deepen, and then destroy the integrity of algal cells. It showed a strong inhibitory effect on algae.
【作者單位】： 中國水產(chǎn)科學(xué)研究院珠江水產(chǎn)研究所農(nóng)業(yè)部熱帶亞熱帶水產(chǎn)資源利用與養(yǎng)殖重點(diǎn)實(shí)驗(yàn)室;上海海洋大學(xué)水產(chǎn)與生命學(xué)院;
【基金】：“十二五”農(nóng)村領(lǐng)域國家科技計(jì)劃課題(2012BAD25B01)
【分類號(hào)】：X172;X52

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本文編號(hào)：2109790