本文選題：三乙胺 + 生物降解��； 參考：《環(huán)境科學(xué)研究》2016年12期

【摘要】：為治理制藥廢水中殘留的難降解有機(jī)污染物TEA(triethylamine,三乙胺),以石家莊某污水處理廠的活性污泥為材料,采用富集培養(yǎng)和選擇培養(yǎng),分離篩選到1株能以TEA為唯一碳源和氮源生長(zhǎng)代謝的降解菌——S6-2.通過(guò)測(cè)定形態(tài)特征、生理生化特性、G+C(堿基對(duì))摩爾百分比及16S rRNA基因序列系統(tǒng)發(fā)育分析,該菌株被鑒定為赤紅球菌(Rhodococcus ruber).采用單一影響因素試驗(yàn)分析菌株S6-2對(duì)TEA的降解特性,結(jié)果表明:菌株S6-2具有較強(qiáng)的TEA降解能力及降解穩(wěn)定性;其最適降解溫度為32℃,最適降解pH為8.0.菌株S6-2可耐受較高濃度TEA,在ρ(TEA)為900 mg/L的培養(yǎng)基中仍能生長(zhǎng);在最適條件下,菌株S6-2對(duì)TEA的降解率為70.7%±1.8%.該株菌在含TEA的無(wú)機(jī)鹽平板上傳代培養(yǎng)15代后,對(duì)TEA的降解率為69.3%±2.5%,說(shuō)明菌株S6-2對(duì)TEA的降解具有穩(wěn)定性.研究顯示,菌株S6-2可作為TEA污染水體生物修復(fù)的潛在資源.
[Abstract]:In order to treat the residual refractory organic pollutants (tea triethylamine and triethylamine) in pharmaceutical wastewater, the activated sludge from a sewage treatment plant in Shijiazhuang was used as the material for enrichment and selective culture. A biodegradable strain, S6-2, was isolated and screened to use tea as the sole carbon source and nitrogen source for growth and metabolism. The strain was identified as Rhodococcus ruberus by determining the morphological characteristics, physiological and biochemical characteristics, G C (base pair) molar percentage and phylogenetic analysis of 16s rRNA gene sequence. A single factor test was used to analyze the degradation characteristics of tea by strain S6-2. The results showed that the strain S6-2 had strong degradation ability and stability of tea, the optimum degradation temperature was 32 鈩，

本文編號(hào)：2035896