本文選題：微藻 + 影響因素�。� 參考：《中國石油大學(xué)(華東)》2015年碩士論文

【摘要】：CO_2等溫室氣體的排放導(dǎo)致溫室效應(yīng)加重,能源危機威脅著人類的全面發(fā)展,所以高效固碳技術(shù)和新型能源的獲得迫在眉睫。微藻可以高效吸收利用CO_2,又可以實現(xiàn)油脂的積累,達(dá)到固碳減排和生產(chǎn)油脂的的雙重目標(biāo),已經(jīng)受到人們的廣泛關(guān)注。論文為了探索利用微藻實現(xiàn)固碳減排及制取生物柴油的可行性,對微藻固碳減排和油脂積累進行了研究。通過稀釋法對微藻進行分離篩選,得到兩種微藻,分別是藍(lán)藻和小球藻。以微藻比生長速率,微藻干重量增加速率和微藻油脂產(chǎn)率為指標(biāo),通過單因素實驗研究了鹽度、pH、接種量、光照強度和溫度對上述兩種微藻生長及油脂積累的影響。藍(lán)藻生長及油脂積累影響因素研究表明,鹽度為15-25g·L-1,pH為6-7,接種量為20%,光照強度為4000lux和溫度為25℃時,藍(lán)藻生長狀況較好;鹽度為25g·L-1,pH為7,接種量為20%,光照強度為4000lux和溫度為25℃時,藍(lán)藻油脂積累狀況較好。小球藻生長及油脂積累影響因素研究表明,鹽度為5g·L-1,pH為7,接種量為20%,光照強度為2000lux和溫度為30℃時,小球藻生長狀況較好;鹽度為15g·L-1,pH為7,接種量為15%,光照強度為2000lux和溫度為30℃時,小球藻油脂積累狀況較好。以微藻油脂產(chǎn)率為指標(biāo),通過響應(yīng)面法對微藻培養(yǎng)條件進行了優(yōu)化。藍(lán)藻培養(yǎng)條件優(yōu)化結(jié)果表明,溫度、光照強度和pH三個因素與藍(lán)藻油脂產(chǎn)率之間數(shù)學(xué)模型回歸顯著。該模型預(yù)測,當(dāng)pH為7.06,光照強度為3850.27lux,溫度為25.67℃,接種量為20%,鹽度為25g·L-1時,藍(lán)藻油脂產(chǎn)率最大,達(dá)到123.656mg·L-1·d-1。驗證模型可得,藍(lán)藻實際油脂產(chǎn)率為123.802mg·L-1·d-1。小球藻培養(yǎng)條件優(yōu)化結(jié)果表明,溫度、鹽度和pH與小球藻油脂產(chǎn)率之間數(shù)學(xué)模型回歸顯著。該模型預(yù)測,當(dāng)溫度為30.19℃,鹽度為15.03g·L-1,pH為6.99,接種量為15%,光照強度為2000lux時,小球藻油脂產(chǎn)率最大,達(dá)到133.036mg·L-1·d-1。驗證模型得知,小球藻實際油脂產(chǎn)率為132.812mg·L-1·d-1。選取小球藻作為后續(xù)實驗藻種,以微藻比生長速率,微藻干重量增加速率和微藻固碳率為指標(biāo),在優(yōu)化條件下對微藻進行培養(yǎng),研究了CO_2含量分別為10%,20%和30%時微藻的固碳能力。結(jié)果表明,在CO_2含量為30%時,小球藻固碳率,比生長速率和干重量增加速率最大,分別為40.78%,0.156d-1和108.9mg·L-1·d-1。最終確定采用“超濾氣浮耦合采收工藝”對微藻進行采收,并研究了微藻的油脂積累能力,其中,微藻油脂積累能力以微藻油脂產(chǎn)率為指標(biāo)。通過測定微藻固碳后油脂產(chǎn)率得出,微藻固碳后,其油脂產(chǎn)率達(dá)到116.732mg·L-1·d-1。綜上所述,微藻在固碳的同時,可以同時積累油脂。所以,可以利用微藻實現(xiàn)固碳減排和生產(chǎn)生物柴油,緩解環(huán)境壓力和能源危機。
[Abstract]:The emission of CO_2 and other greenhouse gases lead to the aggravation of Greenhouse Effect, and the energy crisis threatens the overall development of human beings, so it is urgent to obtain efficient carbon sequestration technology and new energy sources. Microalgae can efficiently absorb and utilize CO-2, and can realize the accumulation of oil, and achieve the dual goals of carbon sequestration and oil production, which has been paid more and more attention. In order to explore the feasibility of using microalgae to reduce carbon sequestration and to produce biodiesel, the carbon reduction and oil accumulation of microalgae were studied. Two kinds of microalgae, cyanobacteria and Chlorella microalgae, were isolated and screened by dilution method. Based on the specific growth rate of microalgae, the increase rate of dry weight of microalgae and the yield of microalgae oil, the effects of pH, inoculation amount, light intensity and temperature on the growth and oil accumulation of microalgae were studied by single factor experiments. The study on the influence factors of cyanobacteria growth and oil accumulation showed that the growth condition of cyanobacteria was better when salinity was 15-25 g / L ~ (-1), pH was 6-7, inoculation amount was 20, light intensity was 4000lux and temperature was 25 鈩，

本文編號：1990530