本文選題：油水淹地 + 表面活性劑產(chǎn)生菌��； 參考：《吉林農(nóng)業(yè)大學(xué)學(xué)報(bào)》2016年06期

【摘要】：從油水淹地污染土壤中獲得26株石油降解菌,篩選出表面活性劑產(chǎn)生菌1株(H-6)和優(yōu)勢(shì)菌6株(H-1、H-17、H-18、H-19、H-20、H-23),結(jié)合菌株形態(tài)觀察、革蘭氏染色和16S r DNA序列同源性分析,鑒定表面活性劑產(chǎn)生菌H-6為堀越氏芽孢桿菌(Bacillus horikoshii)。以表面活性劑產(chǎn)生菌H-6為中心,再任選3株優(yōu)勢(shì)菌株構(gòu)建石油降解菌群,得到高效石油降解菌群C5(H-1、H-6、H-18、H-19),通過正交試驗(yàn)得到菌群C5各菌種的最佳接種量。結(jié)果表明:接種量15%(H-1)、15%(H-6)、20%(H-18)、10%(H-19),溫度25℃,石油含量2 000 mg/L條件下,7 d時(shí)石油降解率達(dá)到78.87%。這說明高效降解菌群C5對(duì)石油具有較好的降解效果,可應(yīng)用于油水淹地污染土壤的修復(fù)。
[Abstract]:26 strains of petroleum degrading bacteria were obtained from contaminated soil under oil flooding, and 1 strain of surfactant producing strain H-6) and 6 strains of dominant bacteria were selected. Combined with morphological observation of strain, Gram staining and homology analysis of 16s r DNA sequence were carried out. The surfactant producing strain H-6 was identified as Bacillus horikoshiiformis. Taking the surfactant producing strain H-6 as the center, 3 dominant strains were selected to construct the petroleum degrading bacteria, and the high-efficiency petroleum degrading bacteria C5H-1H-6H-6H-18H-19 were obtained. The optimum inoculation amount of C5 strains was obtained by orthogonal test. The results showed that the oil degradation rate reached 78.87 at the temperature of 25 鈩，

本文編號(hào)：1942484