本文選題：低溫 + 反硝化菌劑　； 參考：《四川師范大學(xué)》2015年碩士論文

【摘要】：目前水體中的氮素污染已經(jīng)成為我國環(huán)境保護(hù)面臨的一個重要問題。高效經(jīng)濟(jì)穩(wěn)定的脫氮處理技術(shù)及其基礎(chǔ)應(yīng)用研究非常重要。相對于理化法,生物脫氮具有更高的經(jīng)濟(jì)效益,是目前應(yīng)用最廣的技術(shù)。溫度對反硝化的影響非常大,我國北方地區(qū)冬季氣候寒冷,造成該地區(qū)冬季污水處理廠脫氮處理效果不理想。本研究以實(shí)驗(yàn)室已存的三株耐冷反硝化菌LTD-03,LTD-05,LTD-16為研究對象,根據(jù)三株菌的脫氮特性,研制耐低溫反硝化菌劑,考察菌劑的生長特性及低溫脫氮特性。將菌劑用于生活污水的脫氮處理,探討處理時的環(huán)境條件及處理效果�？疾烊昴屠浞聪趸拿摰匦园l(fā)現(xiàn),三株菌的硝氮和總氮去除率LTD-03LTD-16LTD-05;三株菌的亞硝氮積累量LTD-03LTD-16LTD-05。根據(jù)三株菌的這一特性,通過三株菌的混合,研制復(fù)合菌劑,復(fù)合菌劑比例為(LTD-03):(LTD-05):(LTD-16)=2:1:3時,能夠在獲得最大硝酸鹽氮降解速率的同時積累最少的亞硝酸鹽氮,將此復(fù)合菌劑標(biāo)記為菌劑CM。考察CM的生長特性,菌劑CM生長的最佳C/N為20:1;雖然隨著溫度的升高,菌劑CM生長越旺盛,但是初始OD600≈0.40時,接種60h后,OD600≈1.21,菌劑的平均生長速率達(dá)到0.52d-1,菌劑在10℃也能生長良好。CM生長的最佳p H=8,p H=6僅為p H=8時生長速率的1/10;最佳裝液量為400m L,是裝液量為100m L時生長速率的1.69倍。菌劑CM的脫氮特性研究表明,在低溫5~15℃的范圍內(nèi),CM能夠良好地進(jìn)行反硝化,雖然脫氮能力隨著溫度的升高而變強(qiáng),但是在10℃時接種菌劑CM60h后硝氮去除率可達(dá)89.80%;初始硝氮濃度為200mg/L時的硝酸鹽降解速率是50mg/L時的2.26倍;氨氮濃度超過20mg/L會嚴(yán)重抑制菌劑的脫氮能力;菌劑脫氮最佳p H為8,相對于p H為6時硝氮提高了63.91%;最佳C/N為20:1,相對于C/N為5:1硝氮去除率提高了32.47%。菌劑CM在10℃條件下處理生活污水的研究表明,脫氮過程中外加碳源是非常有必要的,添加碳源后硝氮去除率是未添加碳源時的4.11倍。生活污水中原有微生物不會對菌劑CM的脫氮能力造成影響。最佳接種量為3%,比接種量為1%時硝氮去除率提高了40.52%。菌劑CM脫氮的最佳p H為8,相對于p H為6時硝氮去除率提高了73.15%。菌劑CM與活性污泥聯(lián)用能在3h內(nèi)快速去除生活污水中的硝態(tài)氮,去除率達(dá)到100%,相對于未添加菌劑時硝氮去除率提高了23.50%,這種效果有利于脫氮系統(tǒng)在10℃條件下的啟動。我國北方污水處理廠冬季平均水溫一般在10℃左右,菌劑CM能夠在10℃條件下快速穩(wěn)定地去除生活污水中的硝態(tài)氮,具有良好的應(yīng)用前景。項(xiàng)目研究為生活污水的脫氮處理提供了一種新的方法,對我國水污染治理及日益嚴(yán)重的水資源短缺有著重要的意義。
[Abstract]:At present, nitrogen pollution in water has become an important problem in environmental protection in China. It is very important to study the efficient, economical and stable denitrification treatment technology and its basic application. Biological nitrogen removal is the most widely used technology because it has higher economic benefit than physical and chemical method. The effect of temperature on denitrification is very great. The cold winter climate in the northern part of China leads to the unsatisfactory denitrification effect of the wastewater treatment plant in this area in winter. In this study, three strains of cold-tolerant denitrifying bacteria LTD-03N LTD-05LD-16 were used as the research object. According to the denitrification characteristics of three strains of bacteria, the low-temperature denitrification agent was developed, and the growth characteristics and low-temperature denitrification characteristics of the bacteria were investigated. This paper discusses the environmental conditions and treatment effects of domestic wastewater denitrification by using bacteriological agent. The denitrification characteristics of three cold-tolerant denitrifying bacteria were investigated. The results showed that the removal rates of nitrate and total nitrogen were LTD-03LTD-16LTD-05and the accumulation of nitrite was LTD-03LTD-16LTD-05. According to this characteristic of the three strains, through the mixing of the three strains, a compound bacterial agent was developed. When the ratio of the compound agent is LTD-03: 5 / LTD-05: 1: LTD-16: 1: 3, the maximum rate of nitrate nitrogen degradation can be obtained and the least amount of nitrite nitrogen can be accumulated at the same time. The compound bacteria was labeled as CM. The growth characteristics of CM were investigated. The optimum C / N ratio of CM growth was 20: 1. Although the growth of CM increased with the increase of temperature, the initial OD600 鈮，

本文編號：1914145