本文選題：鄰苯二甲酸二(2-乙基)己酯降解菌 + 生物降解　； 參考：《江蘇科技大學(xué)》2015年碩士論文

【摘要】：從污水處理廠活性污泥和垃圾填埋場(chǎng)垃圾滲濾液中分離出3株以鄰苯二甲酸二(2-乙基)己酯(DEHP)為唯一碳源生長(zhǎng)的細(xì)菌菌株S1、S2、S3。通過形態(tài)觀察、生理生化實(shí)驗(yàn)和16S rDNA序列分析等手段對(duì)菌株進(jìn)行了鑒定。研究了這3株菌的生長(zhǎng)特性和降解特性,優(yōu)化了菌株降解DEHP的工藝條件,探討了菌株降解底物的廣譜性。制備了竹炭/聚氨酯復(fù)合材料,并對(duì)其進(jìn)行了表征。將分離的菌株固定到竹炭/聚氨酯復(fù)合材料上,并研究了固定化菌對(duì)DEHP的降解特性。菌株的鑒定和生長(zhǎng)特性研究結(jié)果表明:菌株S1為代爾夫特菌(Delftia sp.),其最適生長(zhǎng)溫度為30℃、pH范圍為7-8,底物濃度為800mg/L;S2為睪丸酮叢毛單胞菌(Comamonas testosterone),其最適生長(zhǎng)溫度為30℃,pH范圍為7-9,底物濃度為400-800mg/L;S3為金黃色桿菌屬(Chryseobacterium sp.),其最適生長(zhǎng)溫度為30℃,pH為8,底物濃度為400mg/L。菌株對(duì)DEHP的降解工藝優(yōu)化結(jié)果表明,菌株S1降解DEHP的適宜條件為:pH為8,溫度30℃,投菌量為5%,底物濃度800mg/L;菌株S2降解DEHP的適宜條件為:pH為8,溫度30℃,投菌量為5%,底物濃度400mg/L;菌株S3降解DEHP的適宜條件為:pH為7,溫度30℃,投菌量為10%,底物濃度400mg/L。在最適條件下,菌株S1能在72h內(nèi)將濃度為800mg/L的DEHP基本降解完,菌株S2能在72h內(nèi)將濃度為400mg/L的DEHP基本降解完,菌株S3降解完400mg/L的DEHP需要120h。菌株降解底物的廣譜性研究結(jié)果表明,三株菌均能有效降解DMP、DEP、DBP和DNOP,培養(yǎng)5d后菌株S1對(duì)DMP、DEP、DBP、DNOP的降解效率均在60%以上,菌株S2對(duì)DMP、DEP、DBP、DNOP的降解效率均在50%以上,菌株S3對(duì)DMP、DEP的降解效率達(dá)70%以上,對(duì)DBP和DNOP的降解率在40%左右。制備的竹炭/聚氨酯復(fù)合材料與聚氨酯相比,化學(xué)結(jié)構(gòu)并未發(fā)生改變,含有大量亞氨基、氨基、羥基等活性化學(xué)基團(tuán),但化學(xué)穩(wěn)定和孔隙率有了明顯提高。3株菌在竹炭/聚氨酯復(fù)合載體上的固定化條件為:以竹炭含量為3%的竹炭/聚氨酯復(fù)合材料作為固定化載體,固定化載體用量為0.025g,最佳固定化時(shí)間是24h。在此固定化條件下,載體對(duì)菌株S1、S2、S3的最大吸菌量分別為216mg/g、204mg/g和212mg/g。菌株S1,S2,S3經(jīng)竹炭/聚氨酯復(fù)合材料固定后,適應(yīng)期明顯縮短,對(duì)DEHP的降解速度和降解效率顯著提高。竹炭/聚氨酯復(fù)合材料是一種固定菌株S1、S2、S3的理想載體。
[Abstract]:Three strains of bacterial strain S1S2OS3 were isolated from activated sludge and landfill leachate of sewage treatment plant, with dioxy 2-ethyl hexyl phthalate (DEHP) as the sole carbon source. The strains were identified by morphological observation, physiological and biochemical experiments and 16s rDNA sequence analysis. The growth and degradation characteristics of the three strains were studied, the process conditions of DEHP degradation were optimized, and the broad-spectrum properties of substrate degradation were discussed. Bamboo charcoal / polyurethane composites were prepared and characterized. The isolated strains were immobilized on bamboo charcoal / polyurethane composites and the degradation characteristics of DEHP by immobilized bacteria were studied. The results of identification and growth characteristics showed that strain S1 was Delftia sp., the optimum growth temperature was 30 鈩，

本文編號(hào)：1891523