東海海域表層沉積物中硫酸鹽還原菌分布特征研究
發(fā)布時間:2018-05-12 15:11
本文選題:東海 + 硫酸鹽還原菌。 參考:《中國環(huán)境科學(xué)》2016年12期
【摘要】:利用2011年4、7、8和10月對東海海域4個航次的調(diào)查資料,以表層沉積物中硫酸鹽還原菌(SRB)為研究對象,針對于SRB所共有的異化型亞硫酸鹽還原酶(DSR)中的β亞基基因(dsr B),通過熒光定量PCR技術(shù)對SRB豐度的時空分布特征進(jìn)行了描述.結(jié)果表明,SRB豐度變化范圍為1.87×10~5~4.69×10~8cells/g,平均值為1.15×10~8cells/g,且4月SRB豐度最低,7月SRB豐度最高;SRB數(shù)量在總細(xì)菌中的比例介于0.0039%~1.6176%之間,說明SRB在東海表層沉積物的細(xì)菌總量中比例很小;SRB豐度的水平分布特征整體表現(xiàn)為南部海域高于北部海域,長江口及浙閩沿岸泥質(zhì)區(qū)高于非泥質(zhì)區(qū).此外,SRB豐度與環(huán)境因子的相關(guān)性分析表明,溫度和溶解氧是影響SRB豐度的重要因素.
[Abstract]:Based on the survey data of 4 voyages in the East China Sea in April 2011 and October 2011, the sulfate reducing bacteria SRB in surface sediments was used as the research object. The spatial and temporal distribution of SRB abundance was described by fluorescence quantitative PCR for the 尾 -subunit gene (DSR) of dissimilated sulfite reductase (DSRs) shared by SRB. The results showed that SRB abundance varied from 1.87 脳 10 ~ (5) to 4.69 脳 10 ~ (8) cells / g with an average value of 1.15 脳 10 ~ (8) cells / g, and the lowest abundance of SRB was observed in April, and the ratio of SRB abundance to total bacteria in July was between 0.0039 and 1.6176%. It is concluded that the horizontal distribution of SRB abundance in the surface sediments of the East China Sea is higher in the southern sea than in the north, and in the muddy areas along the Yangtze Estuary and Zhejiang and Fujian coasts is higher than that in the non-muddy areas. In addition, the correlation analysis between SRB abundance and environmental factors showed that temperature and dissolved oxygen were important factors affecting SRB abundance.
【作者單位】: 中國海洋大學(xué)環(huán)境科學(xué)與工程學(xué)院;海洋環(huán)境與生態(tài)教育部重點(diǎn)實(shí)驗(yàn)室;海洋國家實(shí)驗(yàn)室海洋生態(tài)與環(huán)境科學(xué)功能實(shí)驗(yàn)室;中國海洋大學(xué)海洋生命學(xué)院;
【基金】:國家自然科學(xué)基金(41521064;41620104001) 中國科學(xué)院海洋生態(tài)與環(huán)境科學(xué)重點(diǎn)實(shí)驗(yàn)室 青島海洋科學(xué)與技術(shù)國家實(shí)驗(yàn)室海洋生態(tài)與環(huán)境科學(xué)開放基金(KLMEES201601)
【分類號】:X172
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