本文選題：貧營養(yǎng) + 好氧反硝化細菌　； 參考：《西安建筑科技大學》2015年碩士論文

【摘要】：本研究針對微污染水源水的氮源污染問題,深入研究了貧營養(yǎng)好氧反硝化細菌的脫氮特性,在課題組前期分離的23株貧營養(yǎng)好氧反硝化細菌中篩選并組合得到了高效好氧反硝化功能菌群,討論了優(yōu)勢生態(tài)菌群的最佳投菌量和對微污染水源水的脫氮效能的影響。主要研究結(jié)果包括以下幾個方面:(1)從水庫沉積物中篩選到一株好氧反硝化細菌Acinetobacter sp.Sxf14,對該菌株脫氮特性進行研究,并將其接種到C/N(總有機碳與總氮的比值)為1.2的微污染水庫源水中,以探究其對實際源水總氮的去除效果.結(jié)果顯示:Sxf14能以硝酸鹽和亞硝酸鹽為唯一氮源進行好氧反硝化.反應(yīng)48 h后,NO3--N和NO2--N的去除率分別達74.84±0.86%和40.52±1.49%,TN去除率最高達到65.07±1.56%和41.33±0.98%;72 h內(nèi),微污染水庫源水的TN濃度由2.46±0.02 mg/L降到1.68±0.01 mg/L,去除率達到31.7±0.14%。因此,該菌株具有反硝化能力,能承受較低的碳氮比,降低微污染源水中的氮素,本研究可為微污染水體的菌劑修復(fù)技術(shù)提供科學依據(jù)。(2)采用菌源組合構(gòu)建出優(yōu)勢貧營養(yǎng)好氧反硝化功能菌群GNY(G107+N299+81Y),研究不同碳氮比對組合菌群脫氮性能的影響,結(jié)果表明:隨著碳氮比值的增大,好氧反硝化菌的反應(yīng)速率逐漸增強,功能菌群能夠適應(yīng)貧營養(yǎng)環(huán)境,當C/N為3,硝氮去除率達到57.18%,總氮去除率達到43.52%,且能維持較長時間的好氧反硝化活性。(3)將生態(tài)菌群GNY分別接入水庫純源水和滅菌后的水庫源水小試中,NO3--N最高去除率達到35.9%,TN最高去除率達到46.78%;在純源水培養(yǎng)基中,NO3--N最高去除率達到27.97%,TN最高去除率達到43.58%,投菌組比對照組的硝氮去除率高15.26%,,總氮去除率高17.38%;說明該生物菌群可以適應(yīng)源水貧營養(yǎng)環(huán)境,并能夠有效地降低微污染水體中的氮素。(4)在水庫中層7.5m水深處進行投菌量梯度掛瓶實驗,結(jié)果顯示0.1ppm的投菌量梯度脫氮效果最好,NO3--N去除率最高達到66.49%,TN去除率最高達到53.52%;在水庫源水靜態(tài)投菌量梯度試驗中,0.1ppm投菌量梯度脫氮效果最好,NO3--N和TN去除率最高分別達到41.44%和45.16%,可以得出該生態(tài)菌劑的最佳投菌量梯度為0.1ppm。(5)在原水TOC為6.40mg/L,TN濃度為1.85mg/L條件下,按照0.1ppm菌劑投加量,對貧營養(yǎng)好氧反硝化生態(tài)菌劑進行中試試驗研究,結(jié)果表明,投菌系統(tǒng)的脫氮效果明顯好于對照系統(tǒng),投菌組在系統(tǒng)運行穩(wěn)定后,硝氮去除率維持在43.57%左右,比對照系統(tǒng)高22.34%,總氮去除率維持在51.32%左右,比對照系統(tǒng)高19.34%;與此同時,投菌組生物量明顯高于對照組,好氧反硝化細菌總數(shù)也明顯高于對照組;表明該菌劑可用于微污染水源水處理工程應(yīng)用。
[Abstract]:In order to solve the problem of nitrogen source pollution in micro-polluted source water, the denitrification characteristics of aerobic denitrifying bacteria with poor nutrition were studied. 23 strains of aerobic denitrifying bacteria isolated from the early stage of the research group were screened and combined to obtain the high efficient aerobic denitrification functional flora. The optimal amount of bacteria in the dominant ecological flora and the effect on the denitrification efficiency of the micro-polluted source water were discussed. The main results were as follows: (1) A aerobic denitrifying bacterium Acinetobacter sp. Sxf14 was screened from reservoir sediment, and the denitrification characteristics of this strain were studied. It was inoculated into the source water of micro-polluted reservoir with C / N ratio of 1.2 to explore the removal effect of total nitrogen in real source water. The results showed that: Sxf14 could take nitrate and nitrite as sole nitrogen sources for aerobic denitrification. After 48 h reaction, the removal rates of NO3-N and NO2--N were 74.84 鹵0.86% and 40.52 鹵1.49mg / L, respectively. The highest removal rates of TN were 65.07 鹵1.56% and 41.33 鹵0.9872 h, respectively. The TN concentration of the source water of the micro-polluted reservoir decreased from 2.46 鹵0.02 mg/L to 1.68 鹵0.01mg / L, and the removal rate reached 31.7 鹵0.14g / L. Therefore, the strain has the ability to denitrification, can withstand lower carbon to nitrogen ratio, reduce nitrogen in micro-pollution water, This study can provide scientific basis for microorganism remediation technology of micro-polluted water body. (2) A dominant nutrient deficient aerobic denitrification functional flora GNY(G107 N29981Y was constructed by using microbial source combination, and the effect of different carbon / nitrogen ratio on denitrification performance of the combination was studied. The results showed that the reaction rate of aerobic denitrifying bacteria increased with the increase of C / N ratio, and the functional bacteria could adapt to the poor nutrition environment. When C / N was 3, nitrate removal rate was 57.18, total nitrogen removal rate was 43.52, and aerobic denitrification activity could be maintained for a long time.) Eco-microflora GNY was connected to reservoir pure source water and sterilized reservoir source water respectively to get the highest removal rate of NO3 N. The highest removal rate of TN was 46.78 in pure water medium, the highest removal rate of nitrogen was 27.97% in pure water medium, the removal rate of nitrate nitrogen and total nitrogen was 15.26% and 17.38% higher than that of the control group, which indicated that the bacteria group could adapt to the environment of source water and nutrition, and the total nitrogen removal rate was 17.38% higher than that of the control group, and the highest removal rate of nitrogen was 27.97% in the pure water medium, and the removal rate of nitrate nitrogen was 15.26% and 17.38% higher than that in the control group. And it can effectively reduce the nitrogen content in the slightly polluted water, and carry out the bottling experiment in the depth of 7.5 m water depth of the middle layer of the reservoir. The results showed that 0.1ppm had the best removal rate of no _ 3 N and the highest removal rate of TN was 66.49% and 53.52% respectively, and the highest removal rate of no _ 3 N and TN in static gradient test of reservoir source water was 0.1 ppm. The optimum dosage gradient of the ecological agent is 0.1ppm.mg. 5) when the TOC of raw water is 6.40 mg / L ~ (TN), the concentration of TN is 6.40 mg / L ~ (-1) N ~ (2 +), and the concentration of TN is 6.40 mg 路L ~ (-1) 路L ~ (-1) 路m ~ (-1) ~ (-1). According to the dosage of 0.1ppm, a pilot experiment was carried out on the aerobic and denitrification ecological agent for poor nutrition. The results showed that the denitrification effect of the system was obviously better than that of the control system. The removal rate of nitrate nitrogen was about 43.57%, which was 22.34% higher than that of the control system, and the removal rate of total nitrogen was about 51.32%, which was 19.34% higher than that of the control system, at the same time, the biomass and the total number of aerobic denitrifying bacteria in the inoculated bacteria group were obviously higher than those in the control group. The results show that the microorganism can be used in water treatment engineering of micro-polluted source water.
【學位授予單位】：西安建筑科技大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X52;X172

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