本文選題：好氧反硝化菌 + 分離鑒定�。� 參考：《福建農(nóng)林大學(xué)》2015年碩士論文

【摘要】：氮素是是引起水質(zhì)惡化,導(dǎo)致水體富營(yíng)養(yǎng)化的罪魁禍?zhǔn)字?逐漸成為嚴(yán)重的全球性環(huán)境問題,因而如何有效對(duì)其治理已引起越來越廣泛的關(guān)注。目前,被公認(rèn)為最經(jīng)濟(jì)、有效、也是最有發(fā)展前途的處理方法是生物法。然而,傳統(tǒng)生物脫氮途經(jīng)一般要分別經(jīng)過好氧自養(yǎng)硝化和缺氧異養(yǎng)反硝化兩個(gè)完全獨(dú)立的過程,導(dǎo)致處理過程相對(duì)較復(fù)雜。隨著好氧反硝化菌的發(fā)現(xiàn),硝化反硝化過程可同時(shí)在同一反應(yīng)器內(nèi)進(jìn)行,不僅可減少用地,還大大降低了處理成本。本文從福建農(nóng)林大學(xué)的觀音湖中(富營(yíng)養(yǎng)期)取樣,對(duì)其中的好氧反硝化菌進(jìn)行分離及鑒定,同時(shí)初步研究了其脫氮性能,以其為在氮素污染治理中的應(yīng)用提供數(shù)據(jù)支撐與理論指導(dǎo)。一方面,利用BTB平板的顯色反應(yīng)初行初篩,再經(jīng)過搖瓶復(fù)篩后得到三株具有較高好氧反硝化活性的細(xì)菌,并分別命名為HJH1、HJH2和HJH3。隨后,通過菌種的擴(kuò)大培養(yǎng)、提取DNA、采用PCR擴(kuò)增三菌株的16SrDNA并測(cè)序分析表明,菌株HJH1與Pseudomonas putida strain A5.5的16SrDNA序列相似性為94%,最有可能是假單胞菌屬,并用MP法構(gòu)建了系統(tǒng)進(jìn)化樹。菌株HJH2與Klebsiella oxytoca strain IARI-NIAW2-11同源相似性達(dá)98%,最有可能是克雷伯菌屬,命名為Klebsiella oxytoca strain HJH2;通過16SrDNA基因序列測(cè)定菌株HJH3初步鑒定為分散泛菌屬。另一方面,研究了菌株的好氧反硝化特性、耐受亞硝態(tài)氮濃度以及對(duì)于該菌株反硝化作用的最適pH、溫度、碳源。結(jié)果表明,三株菌株可以有效去除亞硝態(tài)氮,菌株HJH1亞硝態(tài)氮最大耐受濃度為70mg/L,菌株HJH2亞硝態(tài)氮最大耐受濃度為80mg/L,菌株HJH3亞硝態(tài)氮最大耐受濃度為500mg/L；菌株HJH1、 HJH2生長(zhǎng)的最適pH為7,菌株HJH3生長(zhǎng)的最適pH為10；菌株HJH1、HJH2最適溫度為30℃,菌株HJH3的最適合的溫度為37℃；當(dāng)碳源初始濃度為0.1mol/L時(shí),甘油為最合適的碳源；菌株HJH1最適合的碳源為甘油,菌株HJH2最適合的碳源為甘油,菌株HJH3最適合的碳源為丁二酸鈉。
[Abstract]:Nitrogen is one of the main culprits that cause the deterioration of water quality and eutrophication of water, and it has gradually become a serious global environmental problem. Therefore, more and more attention has been paid to the effective management of nitrogen. At present, it is recognized as the most economical, effective, and the most promising treatment method is biological law. However, the traditional biological denitrification process usually goes through two completely independent processes of aerobic autotrophic nitrification and anoxic heterotrophic denitrification, which leads to the relatively complex treatment process. With the discovery of aerobic denitrifying bacteria, the nitrification and denitrification process can be carried out in the same reactor at the same time, which can not only reduce the land use, but also greatly reduce the treatment cost. In this paper, the aerobic denitrifying bacteria were isolated and identified from Guanyin Lake (eutrophic period) of Fujian Agriculture and Forestry University, and their denitrification properties were preliminarily studied. It provides data support and theoretical guidance for the application of nitrogen pollution control. On the one hand, three strains of bacteria with high aerobic denitrification activity were obtained by the initial screening of BTB plate color reaction and then by shaking flask rescreening, and were named HJH1, HJH2 and HJH3, respectively. The 16SrDNA of the three strains was amplified by PCR and sequenced. The results showed that the similarity of 16SrDNA sequence between HJH1 and Pseudomonas putida strain A5.5 was 94g, the most likely was Pseudomonas, and the phylogenetic tree was constructed by MP method. The homology of HJH2 and Klebsiella oxytoca strain IARI-NIAW2-11 was 98%, and the most likely was Klebsiella, named Klebsiella oxytoca strain HJH2, and identified as panbacterium by 16SrDNA gene sequencing. On the other hand, the characteristics of aerobic denitrification, tolerance to nitrite nitrogen concentration and the optimum pH, temperature and carbon source for denitrification of the strain were studied. The results showed that three strains could effectively remove nitrite nitrogen. The maximum tolerance concentration of nitrite nitrogen in strain HJH1 was 70 mg / L, the maximum tolerance concentration of nitrite nitrogen in strain HJH2 was 80 mg / L, the maximum tolerance concentration of nitrite nitrogen in strain HJH3 was 500 mg / L, the optimum pH for the growth of HJH2 was 7, the optimum pH for growth of HJH3 was 10, the maximum tolerance concentration of nitrite nitrogen in strain HJH1 was 70 mg / L, that of strain HJH2 was 80 mg / L, and that of strain HJH3 was 500 mg / L. The optimum temperature of HJH1 and HJH2 is 30 鈩，

本文編號(hào)：1831471