本文選題：好氧同化性硝酸鹽還原適鹽菌 + Photobacterium��； 參考：《山東大學》2015年碩士論文

【摘要】：好氧同化性硝酸鹽還原適鹽菌Photobacterium sp.LP,能在好氧條件下,高效的去除高鹽度有機廢水中的氮,同時可高效去去除COD、磷。從而只需一個反應器便可進行系統(tǒng)運行,這有利于節(jié)省工程基建和能耗費用。Photobacterium sp.LP是南海菌株503-4中不明原因混入的雜菌,經(jīng)篩選分離提純后進行菌種鑒定初步確定為發(fā)光桿菌屬。Photobacterium sp.LP的最佳除氮條件為：鹽度3%、C/NNH4+=13、C/NNO3-=9、pH=7.69、溫度25℃-30℃,DO=7.10mg/L,細菌在初始TN、TP和CODcr分別為109.27mg/L,242.68mg/L,1639.28mg/L的硝酸鹽培養(yǎng)基中,TN、TP和CODcr的最高去除總量分別達到108.24mg/L,32.21mg/L, 1543mg/L;細菌在初始TN、TP和CODcr分別為119.39mg/L,238.44mg/L,1729.16mg/L的銨鹽培養(yǎng)基中,TN、TP和CODCr的最高去除總量分別達到119.31mg/L,34.09mg/L,1707.57mg/L;通過細菌生物氮載量的實驗發(fā)現(xiàn)Photobacterium sp.LP對數(shù)期的生物氮載量比較高,在硝酸培養(yǎng)基與銨鹽培養(yǎng)基中分別可達到85.29 mg/g和103.42 mg/g,可考慮將該細菌的活性污泥處理后作為生物肥料以回收廢水中的氮、磷等。通過銨鹽培養(yǎng)基、硝酸鹽培養(yǎng)基和銨鹽與硝酸鹽混合培養(yǎng)基中氮形態(tài)的轉(zhuǎn)化實驗證明：(1)一定濃度的銨鹽會完全抑制Photobacterium sp.LP的亞硝酸鹽還原作用,抑制但不完全抑制硝酸鹽還原；(2)在整個過程中幾乎沒有含氮氣體的產(chǎn)生,(1)(2)兩點證明該細菌為同化性硝酸鹽還原菌；3. Photobacterium sp.LP對銨鹽的去除是完全基于銨鹽同化作用,該細菌不具有硝化作用通過硝酸鹽還原酶活性試驗,得出了以下結(jié)論：Photobacterium sp.LP的某硝酸鹽還原酶與和該酶相關的對硝酸起還原作用的物質(zhì)位于細胞膜上,為非水解性物質(zhì),該硝酸鹽還原酶的活性受到溫度的影響；Photobacterium sp.LP細胞超聲破碎上清液中不含與NADH, NADPH, reduced methyl viologen有關的硝酸鹽還原酶,不含對硝酸起還原作用的物質(zhì)或不含與其相關的硝酸鹽還原酶酶或兩者都不含有。文中提到的燙海帶鍋爐提碘廢水來自中國山東威海榮成尋山漁業(yè)公司,是一種鹽度≈4.7%,C/N≈20, pH≈2.1的酸性有機廢水,其主要污染物為NH4+、NO2-、N03-、有機氮、COD、磷,而Photobacterium sp.LP是一株適鹽菌,可以耐受較高的鹽度和C/N(在鹽度1.5%到5%的模擬廢水中總氮去除率都可達89.52%以上,在C/N=21時,模擬廢水總氮去除率可達85.93%),并且可同步高效去除COD和總磷,文中生物膜反應器采用的中空纖維膜具有高效截留Photobacterium sp.LP的作用,使反應器中的細菌不會進入出水,本文先將燙海帶鍋爐提碘廢水進行中和和投加磷預處理后接種Photobacterium sp.LP進行了搖瓶試驗,發(fā)現(xiàn)可將廢水處理至TN、TP達到國家綜合污水排放一級標準,CODCr達到國家綜合污水排放二級標準,將細菌Photobacterium sp.LP投加到使用中空纖維膜組件的膜生物反應器中,運用單菌膜生物反應器處理上述預處理后廢水的模擬廢水,反應器長期運行較為良好,穩(wěn)定運行期間可去除大部分污染物,部分運行時間TN、TP、NH4+達到國家綜合污水排放一級標準,CODcr達到國家綜合污水排放二級標準,但是由于單菌沉降性差,在反應器實驗中的排泥含水量過高且膜清洗與更換較為頻繁,造成成本過高等問題而無法投入到實際運用當中。
[Abstract]:The aerobic assimilative nitrate reduction bacteria Photobacterium sp.LP can effectively remove the nitrogen in the high salinity organic wastewater under aerobic conditions, and effectively remove COD and phosphorus, so that only one reactor can be used for system operation, which is beneficial to save engineering construction and energy consumption.Photobacterium sp.LP is the South China Sea strain 503-4 After screening and purification of mixed bacteria, the best nitrogen removal conditions for.Photobacterium sp.LP were identified as: salinity 3%, C/NNH4+=13, C/NNO3-=9, pH=7.69, temperature 25, -30, DO=7.10mg/L, bacteria in initial TN, TP and CODcr respectively for 109.27mg/L, 242.68mg/L, nitric acid. The maximum total removal of TN, TP and CODcr in the salt medium reached 108.24mg/L, 32.21mg/L, and 1543mg/L, respectively, in the initial TN, TP and CODcr respectively of 119.39mg/L, 238.44mg/L, and 1729.16mg/L. It is found that the biological nitrogen load of Photobacterium sp.LP is higher in logarithmic phase, and can reach 85.29 mg/g and 103.42 mg/g respectively in the medium of nitrate and ammonium salt. The active sludge of the bacteria can be considered as a biological fertilizer to recover nitrogen and phosphorus from the wastewater. The transformation experiment of nitrogen form in the salt mixed medium proved that (1) the ammonium salt of a certain concentration could completely inhibit the nitrite reduction of Photobacterium sp.LP, inhibit but not completely inhibit the nitrate reduction; (2) there was almost no nitrogen containing gas in the whole process, and (1) (2) two points proved that the bacteria was an assimilative nitrate reducing bacteria; 3 The removal of ammonium salt by Photobacterium sp.LP is entirely based on ammonium salt assimilation. The bacteria does not have nitrification through nitrate reductase activity test. The following conclusion is drawn: the substance of a nitrate reductase of Photobacterium sp.LP with the enzyme associated with the enzyme is located on the cell membrane and is non hydrolytic. Substance, the activity of the nitrate reductase is affected by the temperature; the Photobacterium sp.LP cell ultrasonic breakup supernatant contains no nitrate reductase related to NADH, NADPH, reduced methyl viologen, and does not contain any substance that is reduced to nitric acid or contains no related nitrate reductase or both. The iodine wastewater from the scalding kelp boiler comes from the Rongcheng Weihai Rongcheng fishing company in Rongcheng, Shandong, China. It is a kind of acid organic wastewater with salinity 4.7%, 20, and 2.1. The main pollutants are NH4+, NO2-, N03-, organic nitrogen, COD, phosphorus, while Photobacterium sp.LP is a strain of salt and can tolerate high salinity and C/N (from 1.5% to 5% in salinity). The total nitrogen removal rate in the simulated wastewater can reach more than 89.52%. At C/N=21, the total nitrogen removal rate of simulated wastewater can reach 85.93%), and the removal of COD and total phosphorus can be effectively removed. The hollow fiber membrane used in the biofilm reactor has the effect of efficient interception of Photobacterium sp.LP, so that the bacteria in the reactor will not enter the effluent. This article will first be ironed in this paper. The iodine wastewater of the kelp boiler was inoculated with Photobacterium sp.LP after neutralization and phosphorous pretreatment to carry out the shaking flask test. It was found that the wastewater could be treated to TN and TP reached the first grade standard of national comprehensive sewage discharge. CODCr reached the two level standard of national comprehensive sewage discharge, and the bacterial Photobacterium sp.LP was added to the hollow fiber membrane module. In the membrane bioreactor, a single membrane bioreactor is used to treat the simulated wastewater of the pretreated wastewater. The reactor has a good long-term operation. During the stable operation, most of the pollutants can be removed. Part of the operation time TN, TP, NH4+ reach the first level standard of national comprehensive sewage discharge, and CODcr reaches the two level standard of national comprehensive sewage discharge. However, due to the poor settlement of mono bacteria, the water content of the sludge in the reactor experiment is too high and the membrane cleaning and replacement is more frequent, which causes the problem of excessive cost and can not be put into practice.

【學位授予單位】：山東大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X172;X703

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