本文選題：微生物制劑 + 硝化細菌　； 參考：《青島理工大學》2016年碩士論文

【摘要】：進入二十一世紀以來,我國內(nèi)陸水產(chǎn)養(yǎng)殖業(yè)迅猛發(fā)展,高密度集約化養(yǎng)殖模式帶來了較大的養(yǎng)殖產(chǎn)出,同時也由于養(yǎng)殖水體排泄物和殘餌的積累以及生物殘骸的沉積,導(dǎo)致氨氮、亞硝酸鹽含量升高,不僅使養(yǎng)殖產(chǎn)量減少,而且可能造成養(yǎng)殖水體微生態(tài)平衡破壞。生物法具有高效、環(huán)保、低成本、無二次污染等特點,已被廣泛使用。微生物制劑能夠?qū)B(yǎng)殖水體有毒的氨氮、亞硝酸鹽轉(zhuǎn)化為無害的硝酸鹽,從而起到凈化水質(zhì)的作用。因此,探尋不同菌種、不同劑量的微生物制劑以及能夠使其吸附迅速生長繁殖的濾料對水產(chǎn)養(yǎng)殖業(yè)的發(fā)展具有重要的意義。本研究建立一種凈化養(yǎng)殖水體裝置,分別投加硝化細菌、芽孢桿菌以及兩者復(fù)合菌劑,并在此裝置的基礎(chǔ)上添加底沙和硝化毛球,探討不同處理組對養(yǎng)殖水體中氨氮、亞硝酸鹽的降解效果和青蝦生長狀況的影響。通過研究得出以下結(jié)論:(1)在溫度25℃、pH7.5、溶解氧6mg/L養(yǎng)殖環(huán)境下,向青蝦養(yǎng)殖系統(tǒng)中分別添加硝化細菌制劑、芽孢桿菌制劑及硝化細菌和芽孢桿菌復(fù)合菌劑。經(jīng)過30天養(yǎng)殖,結(jié)果顯示,對氨氮去除效果:硝化細菌+芽孢桿菌組硝化細菌組芽孢桿菌組空白組;對亞硝酸鹽去除效果:硝化細菌+芽孢桿菌組芽孢桿菌組硝化細菌組空白組;青蝦平均體重硝化細菌+芽孢桿菌組增長最多,硝化細菌組稍大于芽孢桿菌組,空白組最少。(2)在溫度25℃、pH7.5、溶解氧6mg/L養(yǎng)殖環(huán)境下,向青蝦養(yǎng)殖系統(tǒng)中分別添加一倍硝化細菌制劑和兩倍硝化細菌制劑。經(jīng)過30天養(yǎng)殖,結(jié)果顯示,對氨氮去除效果:二倍硝化細菌組硝化細菌組空白組;對亞硝酸鹽去除效果:二倍硝化細菌組硝化細菌組空白組,但二倍硝化細菌組去除效果跟硝化細菌組相比較不成倍數(shù)關(guān)系;青蝦平均體重二倍硝化細菌組增長最多,硝化細菌組次之,空白組最少。(3)在溫度25℃、pH7.5、溶解氧6mg/L養(yǎng)殖環(huán)境下,分別設(shè)置硝化細菌+底沙組、硝化細菌組、底沙組和空白組。經(jīng)過30天養(yǎng)殖,結(jié)果顯示,對氨氮去除效果:硝化細菌+底沙組硝化細菌組底沙組空白組;對亞硝酸鹽去除效果:硝化細菌+底沙組硝化細菌組底沙組空白組;青蝦平均體重硝化細菌+底沙組增長最多,硝化細菌組多于底沙組,空白組最少。(4)在溫度25℃、pH7.5、溶解氧6mg/L養(yǎng)殖環(huán)境下,分別設(shè)置硝化細菌+硝化毛球組、硝化細菌組和空白組。經(jīng)過30天養(yǎng)殖,結(jié)果顯示,對氨氮去除效果:硝化細菌+硝化毛球組硝化細菌組空白組;對亞硝酸鹽去除效果:硝化細菌+硝化毛球組硝化細菌組空白組;青蝦平均體重硝化細菌+硝化毛球組增長最多,硝化細菌組次之,空白組最少。(5)在屬水平上,硝化細菌組各菌種所占比例比較均勻,其中主要優(yōu)勢菌群為Phaeodactylibacter和Donghicola,所占比例分別為13.69%和13.23%;其次為黃桿菌屬(Tenacibaculum)和浮霉狀菌屬(Planctomyces),分別占9.80%和7.10%。硝化細菌+芽孢桿菌組主要優(yōu)勢菌群為Phaeodactylibacter,所占比例超過70%;另外噬冷彎曲菌屬(Psychroflexus)和Maritimibacter所占比例分別為2.89%和2.09%。兩者都存在優(yōu)勢菌種Phaeodactylibacter·xiamenensis和Phaeodactylibacter·luteus。
[Abstract]:Since twenty-first Century, the inland aquaculture industry has developed rapidly in China, and the high density intensive farming model has brought large production output. At the same time, the accumulation of excrement and residual bait and the deposition of biological debris have resulted in the increase of ammonia nitrogen and nitrite, which not only reduces the production of aquaculture, but also causes the cultivation of aquaculture. The biological method has the characteristics of high efficiency, environmental protection, low cost, no two pollution and so on. It has been widely used. The microbial agent can convert the toxic ammonia nitrogen and nitrite into harmless nitrate in the aquaculture water, so as to purify the water quality. And the filter material which can make it adsorbed and propagate rapidly is of great significance to the development of aquaculture. In this study, we set up a purifying water body, adding nitrifying bacteria, Bacillus spore and both compound bacteria, and adding bottom sand and nitrifying ball on the basis of this device, and discussed the different treatment groups in the aquaculture water body. The effects of ammonia nitrogen and nitrite degradation and the growth status of shrimp were studied. The following conclusions were obtained: (1) nitrifying bacteria preparation, bacillus preparation, nitrifying bacteria and Bacillus spore compound bacteria were added to the aquaculture system at 25, pH7.5, and dissolved oxygen in the culture environment. After 30 days, the results showed that Ammonia nitrogen removal efficiency: nitrifying bacteria + bacillus group nitrifying bacteria group blank group, nitrite removal effect: nitrifying bacteria + Bacillus spore group nitrifying bacteria group group, the average weight of nitrifying bacteria + bacillus group increased most, nitrifying bacteria group was slightly larger than the bacillus group, the blank group was the most. Less. (2) under the environment of temperature 25, pH7.5 and dissolved oxygen 6mg/L, the nitrifying bacteria preparation and two times nitrifying bacteria preparation were added to the shrimp culture system respectively. After 30 days culture, the results showed that the ammonia nitrogen removal effect was two times the nitrifying bacteria group empty white group of nitrifying bacteria group, and the nitrite removal effect was two times nitrifying nitrite. The removal efficiency of the two times nitrifying bacteria group was not related to the nitrifying bacteria group, the average weight of the two times the nitrifying bacteria group increased most, the nitrifying bacteria group was the group, and the blank group was the least. (3) the nitrifying bacteria + bottom sand group and the nitrifying bacteria group were set up under the temperature 25, pH7.5 and dissolved oxygen 6mg/L culture environment. After 30 days of aquaculture, the results showed that the removal efficiency of ammonia nitrogen: nitrifying bacteria + bottom sand group nitrifying bacteria group bottom sand group blank group, nitrite removal effect: nitrifying bacteria + bottom sand group nitrifying bacteria group bottom sand group blank group, the average weight of nitrifying bacteria + bottom sand group of the green shrimp increased most, nitrifying bacteria group more than the bottom sand group. The blank group was the least. (4) under the temperature 25, pH7.5 and dissolved oxygen 6mg/L, the nitrifying bacteria + nitrifying ball group, the nitrifying bacteria group and the blank group were set respectively. After 30 days culture, the results showed that the ammonia nitrogen removal efficiency was: nitrifying bacteria + nitrifying ball group nitrifying bacteria group blank group; nitrite removal effect: nitrifying bacteria + nitrifying ball The average weight of nitrifying bacteria + nitrifying ball in the group of nitrifying bacteria increased most and the nitrifying bacteria group was the least. (5) at the level of the genus, the proportion of the bacteria in the nitrifying bacteria group was more uniform, the main dominant bacteria group was Phaeodactylibacter and Donghicola, the proportion was 13.69% and 13.23% respectively. Secondly, the proportion of the bacteria was 13.23%. The genus (Tenacibaculum) and buoy genus (Planctomyces) accounted for 9.80% and 7.10%. nitrobacteria + bacillus and Bacillus spore group Phaeodactylibacter, which accounted for more than 70%, and the proportion of phagocytosis (Psychroflexus) and Maritimibacter was 2.89% and 2.09%., respectively, both of which had the dominant strain Phaeodac. Tylibacter. Xiamenensis and Phaeodactylibacter. Luteus.

【學位授予單位】：青島理工大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：X52

【參考文獻】

相關(guān)期刊論文 前10條

1 秦健;周東;王亞宜;劉山虎;潘綿立;黎力;宋成康;;綜合多種檢測方法分析鹽度對硝化菌活性的影響[J];中國給水排水;2014年05期

2 李永;楊其彬;蘇天鳳;周發(fā)林;楊麗詩;黃建華;;氨氮對斑節(jié)對蝦的毒性及免疫指標的影響[J];上海海洋大學學報;2012年03期

3 劉道玉;吳偉;;水產(chǎn)養(yǎng)殖水體污染及微生物修復(fù)的研究[J];現(xiàn)代農(nóng)業(yè)科技;2011年17期

4 李久安;周后珍;劉慶華;譚周亮;李旭東;;廢水生物強化處理技術(shù)研究進展[J];應(yīng)用與環(huán)境生物學報;2011年02期

5 李芳芳;齊樹亭;石玉新;呂玉珊;高長虹;;新型固定化硝化細菌和好氧反硝化細菌處理氨氮廢水[J];生態(tài)科學;2010年05期

6 梁擁軍;孫向軍;楊璞;劉金蘭;楊廣;;硝化細菌在澳洲銀鱸工廠化養(yǎng)殖中的應(yīng)用初探[J];安徽農(nóng)業(yè)科學;2009年01期

7 李長玲;黃翔鵠;李瑞偉;王輝;區(qū)啟鴻;;硝化細菌改善魚苗培育環(huán)境增強羅非魚抗逆性研究[J];漁業(yè)現(xiàn)代化;2008年05期

8 王建芳;趙慶良;林佶侃;金文標;;生物強化技術(shù)及其在廢水生物處理中的應(yīng)用[J];環(huán)境工程學報;2007年09期

9 曲克明;徐勇;馬紹賽;李健;;不同溶解氧條件下亞硝酸鹽和非離子氨對大菱鲆的急性毒性效應(yīng)[J];海洋水產(chǎn)研究;2007年04期

10 吳彥彬;李亞丹;李小俊;邊艷青;;擬桿菌的研究及應(yīng)用[J];生物技術(shù)通報;2007年01期

，

本文編號：1784117