本文選題：厭氧氨氧化 + 諾氟沙星�。� 參考：《大連理工大學(xué)》2015年碩士論文

【摘要】：厭氧氨氧化(Anaerobic ammonium oxidation)技術(shù)是一種新型的生物脫氮技術(shù),它的反應(yīng)原理是在厭氧或缺氧條件下,厭氧氨氧化微生物以亞硝酸氮為電子受體,以氨氮為電子供體,將兩者轉(zhuǎn)化為氮?dú)�。該技術(shù)具有不會(huì)產(chǎn)生溫室氣體、無需曝氣和無需添加碳源等優(yōu)點(diǎn)。利用厭氧氨氧化技術(shù)低耗處理實(shí)際廢水已成為近年來環(huán)境領(lǐng)域的一大研究熱點(diǎn)。但是,由于抗生素的大量使用,除制藥廢水、醫(yī)療廢水和蓄禽養(yǎng)殖廢水外,甚至是生活污水中均檢測(cè)到抗生素的存在。由于各類污水中抗生素的存在不可避免,因此,有必要進(jìn)行抗生素對(duì)厭氧氨氧化菌的活性抑制研究。本課題的主要研究方向是考察實(shí)際廢水中含量較高的氟喹諾酮類抗生素諾氟沙星和左氧氟沙星對(duì)厭氧氨氧化菌活性的抑制作用。通過短期實(shí)驗(yàn)和長(zhǎng)期的抑制實(shí)驗(yàn),考察了上述兩種抗生素對(duì)厭氧氨氧化菌活性的抑制效果。主要研究結(jié)果如下：(1)在短期實(shí)驗(yàn)中,當(dāng)諾氟沙星的添加濃度達(dá)200 mg/L時(shí),總氮脫除速率從0.546 mgN/g VSS/h降至0.315 mg N/g VSS/h,是空白對(duì)照組的57.6%。當(dāng)左氧氟沙星添加濃度為400mg/L時(shí),總氮脫除效率從0.762 mg N/g VSS/h降至0.4 mg N/g VSS/h,是空白對(duì)照組的52.5%。(2)諾氟沙星的半抑制濃度(ICs0)為227.39 mg/L,左氧氟沙星的IC50為386.75 mg/L,諾氟沙星對(duì)厭氧氨氧化污泥的抑制作用強(qiáng)于左氧氟沙星。(3)在長(zhǎng)期實(shí)驗(yàn)中,當(dāng)進(jìn)水中諾氟沙星的濃度達(dá)10 μg/L時(shí),不會(huì)對(duì)厭氧氨氧化污泥產(chǎn)生較為明顯的抑制效果,脫氮百分比和總氮脫除率均值分別為76.75%和0.586kgN/m3/d,與對(duì)照組的78.59%和0.599 kg N/m3/d相比較為接近。(4)在長(zhǎng)期實(shí)驗(yàn)中,當(dāng)進(jìn)水諾氟沙星的濃度從25增至50 mg/L時(shí),不會(huì)對(duì)厭氧氨氧化污泥的活性產(chǎn)生明顯的抑制效果,當(dāng)諾氟沙星濃度達(dá)75 mg/L時(shí),厭氧氨氧化污泥的活性受到明顯抑制,脫氮百分比和總氮脫除率分別下降至50.51%和0.445 kg N/m3/d。(5)在長(zhǎng)期實(shí)驗(yàn)中,當(dāng)進(jìn)水濃度始終保持150 mg/L時(shí),會(huì)對(duì)厭氧氨氧化污泥的活性產(chǎn)生明顯的抑制效果,反應(yīng)器運(yùn)行36 d后完全崩潰,脫氮百分比和總氮脫除率分別下降至8.34%和0.073 kg N/m3/d。
[Abstract]:Anaerobic ammonia oxidation (anaerobic ammonium oxidation) technique is a new biological denitrification technique. Its reaction principle is that the anaerobic ammonia-oxidizing microorganism takes nitrite nitrogen as electron receptor and ammonia nitrogen as electron donor to convert both into nitrogen under anaerobic or anoxic conditions.The technology has the advantages of no greenhouse gas, no aeration and no carbon source.Using anaerobic ammonia oxidation technology to treat practical wastewater with low consumption has become a research hotspot in the field of environment in recent years.However, due to the extensive use of antibiotics, antibiotics are detected in the domestic sewage, except pharmaceutical wastewater, medical wastewater and poultry storage wastewater.Because of the inevitable existence of antibiotics in various kinds of sewage, it is necessary to study the activity inhibition of antibiotics to anaerobic ammonia oxidizing bacteria.The main research direction of this paper is to investigate the inhibitory effect of norfloxacin and levofloxacin on the activity of anaerobic ammonia oxidizing bacteria with high content of fluoroquinolones in actual wastewater.The inhibitory effects of these two antibiotics on the activity of anaerobic ammonia-oxidizing bacteria were investigated by short-term and long-term inhibition experiments.The main results were as follows: (1) in the short-term experiment, when the concentration of norfloxacin reached 200 mg/L, the removal rate of total nitrogen decreased from 0.546 mgN/g VSS/h to 0.315 mg N / g VSS / h, which was 57.6% of the control group.When levofloxacin was added with a concentration of 400mg/L,The total nitrogen removal efficiency decreased from 0.762 mg N / g VSS/h to 0.4 mg N / g VSS / h, which was 52.5% of the blank control group. ICs0) of norfloxacin was 227.39 mg / L, the IC50 of levofloxacin was 386.75 mg / L, and the inhibitory effect of norfloxacin on anaerobic ammonia oxidation sludge was stronger than that of levoxacin on anaerobic ammonia oxidation sludge.In long-term experiments,When the concentration of norfloxacin in the influent reaches 10 渭 g / L, the anaerobic ammonia oxidation sludge will not be inhibited significantly.The average denitrification percentage and total nitrogen removal rate were 76.75% and 0.586kgN / m ~ (3 / d) respectively, which were similar to those of the control group (78.59% and 0.599 kg N/m3/d, respectively.) in the long-term experiment, when the concentration of norfloxacin in the influent increased from 25 to 50 mg/L,When the concentration of norfloxacin reached 75 mg/L, the activity of anaerobic ammonia oxidized sludge was significantly inhibited.The percentage of nitrogen removal and the removal rate of total nitrogen decreased to 50.51% and 0.445 kg N / m ~ 3 / d 路m ~ (5) respectively. In a long-term experiment, when the influent concentration was kept at 150 mg/L, the activity of anaerobic ammonia oxidized sludge was inhibited obviously, and the reactor completely collapsed after 36 days of operation.The percentage of nitrogen removal and the removal rate of total nitrogen decreased to 8.34% and 0.073 kg N / m 3 / d, respectively.
【學(xué)位授予單位】：大連理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X703

【共引文獻(xiàn)】

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