本文選題：抗生素耐藥基因 + 宏基因組分析　； 參考：《中山大學學報(自然科學版)》2017年02期

【摘要】：細菌對抗生素的耐藥性是全球關(guān)注的環(huán)境健康問題之一。該研究旨在使用宏基因組方法比較分析西太平洋深海和珠江口沉積物中抗生素耐藥基因,認識抗生素使用與環(huán)境細菌耐藥性間的關(guān)系。研究發(fā)現(xiàn),深海沉積物多重耐藥基因的含量高達77.8%;珠江口沉積物多重耐藥基因只有27.2%,常用抗生素的耐藥基因(磺胺類、大環(huán)內(nèi)酯類、氨基糖苷類等)含量明顯提高(約70%)。沉積物中共發(fā)現(xiàn)45種耐藥基因亞型,其中7種基因亞型(acrB、amrB、bacA、ceoB、macB、mexB和smeE)能在所有沉積物中發(fā)現(xiàn)。深海沉積物中質(zhì)粒僅攜帶0.3%的耐藥基因,而珠江口沉積物則超過40%。研究表面,由于珠江口周邊區(qū)域常用抗生素的廣泛使用,其沉積物中細菌抗生素耐藥性明顯提高、耐藥機制趨于多樣化。
[Abstract]:Bacterial resistance to antibiotics is one of the global environmental health concerns.The purpose of this study was to compare and analyze antibiotic resistance genes in sediments from the deep sea of the western Pacific Ocean and the Pearl River Estuary by using a macro genome method and to understand the relationship between antibiotic use and environmental bacterial resistance.It is found that the content of multidrug resistance genes in deep-sea sediments is as high as 77.8, and that in sediments of Pearl River Estuary is only 27.2.The content of commonly used antibiotic resistant genes (sulfonamides, macrolides, aminoglycosides, etc.) is obviously increased (about 70%).A total of 45 subtypes of drug resistance genes were found in sediments, of which 7 were found in all sediments.The plasmids contained only 0.3% of drug resistance genes in deep sea sediments, but more than 40% in Pearl River Estuary sediments.Because of the extensive use of common antibiotics in the area around the Pearl River Estuary, the antibiotic resistance of bacteria in the sediments increased obviously, and the mechanism of drug resistance tended to be diversified.
【作者單位】： 南方醫(yī)科大學公共衛(wèi)生學院;南方醫(yī)科大學珠江醫(yī)院;中山大學孫逸仙紀念醫(yī)院;中山大學海洋學院;
【基金】：廣東省自然科學基金(2014A030313195)
【分類號】：X55

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