本文選題：聚吡咯　切入點：電極改性　出處：《大連理工大學》2015年碩士論文

【摘要】：電極生物膜法作為將生物膜法和電化學法聯(lián)合而發(fā)展起來的污水處理技術,近年來越來越受到研究者們的關注。其所具有的優(yōu)勢有：生物膜微生物固定生長且生物量較大；電化學電極過程擁有高效的氧化還原能力；電極與微生物之間具有較高傳質(zhì)速率。隨著生物電化學反應器的發(fā)展,涉及到生物電化學反應器的電極改性被廣泛研究。有研究報道,改性電極運用到生物電化學反應器中可以提高反應器性能。本論文探究利用電化學聚合方法在碳纖維氈電極上制備聚吡咯(polypyrole, PPy)的聚合效果,并利用傅里葉變換紅外光譜(Fourier transform infrared, FTIR)及掃描電鏡(SEM)對其進行表征；將改性后的碳纖維氈電極應用到電極生物膜反應器(Biofilm Electrode Reactor, BER)中,考察電極改性對電極生物膜反應器性能的影響并研究聚吡咯覆膜改性電極對生物膜附著量、胞外聚合物成分及生物膜微生物群落的影響。結(jié)果表明,以25 mM AQDS(蒽醌2,6-二磺酸鈉)為摻雜劑的恒電壓電化學聚合能夠在碳纖維電極表面形成均勻穩(wěn)定的聚吡咯膜,實現(xiàn)聚吡咯在碳纖維氈電極上的覆膜改性。改性后的電極應用到電極生物膜反應器中,可使自養(yǎng)反硝化反應器對N03--N的去除效率由對照反應器的67.3%增加到83.9%,處理效果提高了24.7%；可使產(chǎn)甲烷反應器COD去除率較其對照反應器最高增加約18%,甲烷產(chǎn)量較其增加了約3倍。對兩種反應器內(nèi)電極生物膜進行生物量測定和掃描電鏡分析,可以看到應用聚吡咯覆膜改性電極的R2反應器中改性電極生物膜附著量均明顯多于對照反應器R1中未改性電極生物膜的附著量,說明電極改性有利于生物膜的附著。生物膜胞外聚合物蛋白質(zhì)和多糖的測定結(jié)果說明,電極改性有助于提高胞外聚合物的含量和蛋白質(zhì)及多糖的比例,從而提高生物膜的活性及其強度和穩(wěn)定性。氫自養(yǎng)反硝化電極生物膜微生物16S rDNA分析結(jié)果表明聚吡咯覆膜改性電極生物膜的優(yōu)勢菌為Hydrogenophaga sp(噬氫菌屬)和Thauera sp(陶厄氏菌屬),不同于對照電極生物膜菌落組成中的優(yōu)勢菌屬Dechloromonas sp.,并且其生物膜的菌落組成更多樣化。產(chǎn)甲烷電極生物膜微生物古菌16S rDNA分析結(jié)果表明兩反應器電極生物膜菌落組成中優(yōu)勢菌屬均為Methanosarcina sp(產(chǎn)甲烷八疊球菌),聚吡咯覆膜改性電極生物膜的優(yōu)勢菌屬其次為Methanomicrobia sp(產(chǎn)甲烷微菌),而對照電極生物膜優(yōu)勢菌屬其次為Methanobacterium sp(產(chǎn)甲烷桿菌),兩反應器微生物菌屬組成有明顯差異。這說明電極材料的改性對電極生物膜微生物群落的組成產(chǎn)生了影響。
[Abstract]:Electrode biofilm, as a wastewater treatment technology which combines biofilm and electrochemical process, has attracted more and more attention in recent years.Its advantages are: biofilm microorganism growth and biomass is large; electrochemical electrode process has high redox ability; electrode and microorganism have a higher rate of mass transfer.With the development of bioelectric chemical reactor, electrode modification related to bioelectrochemical reactor has been widely studied.It has been reported that the modified electrode can improve the performance of the reactor.In this paper, the polymerization effect of polypyrole (Pyrrolidine) on carbon fiber felt electrode by electrochemical polymerization was investigated and characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).The modified carbon fiber felt electrode was applied to Biofilm Electrode Reactor (ber). The effect of electrode modification on the performance of electrode biofilm reactor was investigated and the adhesion of polypyrrole film modified electrode to biofilm was studied.Effects of extracellular polymer composition and biofilm microbial community.The results show that the uniform and stable polypyrrole film can be formed on the surface of carbon fiber electrode by constant voltage electrochemical polymerization with 25 mm AQDS as dopant, and the modification of polypyrrole film on carbon fiber felt electrode can be realized.The modified electrode is applied to the electrode biofilm reactor.The removal efficiency of N03-N in autotrophic denitrification reactor was increased from 67.3% to 83.9% in the control reactor, and the treatment effect was increased by 24.70.The removal rate of COD in the methanogenic reactor was increased by about 18%, and the methane production was increased about 3 times compared with the control reactor.The biofilm of electrode in two kinds of reactors was measured and analyzed by scanning electron microscope (SEM).The results show that the modification of electrode can improve the content of extracellular polymer and the ratio of protein and polysaccharide, thus improving the activity, strength and stability of biofilm.The results of 16s rDNA analysis of biofilm on hydrogen autotrophic denitrifying electrode indicate that the dominant bacteria of polypyrrole coated electrode biofilm are Hydrogenophaga spp and Thauera spspia, which are different from those of control electrode biofilm colony composition.The dominant bacteria were Dechloromonas sp., and its biofilm colony composition was more diversified.It was Methanomicrobia spp (methanogenic microorganism), while the dominant biofilm bacteria in the control group was Methanobacterium spp (methanogenic bacteria), and the composition of the two biofilms was obviously different between the two reactors.This indicates that the modification of electrode material has an effect on the composition of microbial community of electrode biofilm.
【學位授予單位】：大連理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X703

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