本文選題：抗生素　切入點：大腸桿菌　出處：《生態(tài)毒理學(xué)報》2017年01期

【摘要】：以瓊脂稀釋法對67株雞源大腸桿菌及61株豬源大腸桿菌進行最低抑菌濃度(MIC)測定,并以SPSS分別進行卡方檢驗和Probit模型估算,分析不同來源大腸桿菌耐藥差異顯著性及不同抗生素的半數(shù)抑菌濃度(MIC50),以期對不同畜禽糞便來源的大腸桿菌耐藥差異進行詳細準確的探討。結(jié)果顯示,雞源大腸桿菌對頭孢噻肟、諾氟沙星、環(huán)丙沙星和四環(huán)素耐藥率分別為98.51%、68.66%、56.72%和100%,豬源大腸桿菌對頭孢噻肟耐藥率為88.52%,對諾氟沙星、環(huán)丙沙星和四環(huán)素則100%耐藥。除四環(huán)素,雞源、豬源大腸桿菌對諾氟沙星、環(huán)丙沙星、頭孢噻肟的耐受差異顯著(P0.05),51%的雞源大腸桿菌和89%的豬源大腸桿菌均呈4重耐藥。SPSS分析結(jié)果表明,Probit模型估算結(jié)果優(yōu)于當(dāng)前MIC50常規(guī)計算方法,頭孢噻肟、諾氟沙星、環(huán)丙沙星和四環(huán)素對雞源大腸桿菌MIC50分別為40.031μg·m L~(-1)、40.020μg·m L~(-1)、2.683μg·m L~(-1)和101.418μg·m L~(-1),對豬源大腸桿菌MIC50分別為8.724μg·m L~(-1)、56.044μg·m L~(-1)、31.214μg·m L~(-1)和130.915μg·m L~(-1)�；貧w方程顯示環(huán)丙沙星對雞源大腸桿菌抑制作用最強;高于40.031μg·m L~(-1)時,諾氟沙星抗菌作用弱于頭孢噻肟,低于120.23μg·m L~(-1)時,諾氟沙星抗菌作用強于四環(huán)素;頭孢噻肟、環(huán)丙沙星、諾氟沙星和四環(huán)素對豬源大腸桿菌的抑菌作用則呈遞減趨勢。同時驗證了諾氟沙星和環(huán)丙沙星屬同種作用機制,基于Probit模型計算更簡單、快速、直觀。研究結(jié)果可為畜禽養(yǎng)殖中大腸桿菌的耐藥差異監(jiān)控提供一定的數(shù)據(jù)基礎(chǔ)。
[Abstract]:Minimum inhibitory concentration of 67 Escherichia coli and 61 strains of Escherichia coli by agar dilution method (MIC) and SPSS were determined by chi square test and Probit model to estimate and analysis of half inhibitory concentration of different sources of drug resistance of Escherichia coli and significantly different antibiotics (MIC50), for different livestock manure the source of the drug resistance of Escherichia coli in order to explore the differences of detail and accurate. The results showed that Escherichia coli of norfloxacin, cefotaxime, ciprofloxacin and tetracycline resistance rates were 98.51%, 68.66%, 56.72% and 100%, Escherichia coli cefetaxime resistant rate was 88.52%, of norfloxacin, ciprofloxacin and tetracycline were 100% resistance. Except for tetracycline, ciprofloxacin in chicken, Escherichia coli, tolerance to norfloxacin, cefotaxime was significantly difference (P0.05), Escherichia coli 51% Escherichia coli and 89% were 4閲嶈，

本文編號：1664225