本文選題：水稻　切入點(diǎn)：Cd-K雙重處理　出處：《植物資源與環(huán)境學(xué)報(bào)》2016年04期 　論文類型：期刊論文

【摘要】：以水稻(Oryza sativa Linn.)高Cd積累品種‘T優(yōu)705’(‘T You 705’)和低Cd積累品種‘湘早秈24’(‘Xiangzaoxian 24’)為實(shí)驗(yàn)材料,采用水培法對(duì)不同濃度Cd(0.0和2.7μmol·L~(- 1)Cd)和K(0、30和60 mmol·L~(- 1)K)處理?xiàng)l件下2個(gè)品種幼苗的相對(duì)生長(zhǎng)量、根系和地上部的Cd含量及其亞細(xì)胞分布特征進(jìn)行了比較,并分析了添加離子通道活性抑制劑TEA和La Cl3后幼苗根系和地上部的Cd和K含量;在此基礎(chǔ)上,比較了NSCCs(非選擇性陽離子通道)和K專性通道對(duì)2個(gè)品種幼苗根系和地上部Cd和K吸收貢獻(xiàn)率的影響。結(jié)果表明:與Cd單一處理組(2.7μmol·L~(- 1)Cd)相比,Cd-K雙重處理組(2.7μmol·L~(- 1)Cd-30 mmol·L~(- 1)K和2.7μmol·L~(- 1)Cd-60 mmol·L~(- 1)K)2個(gè)品種幼苗的相對(duì)生長(zhǎng)量顯著提高,而幼苗根系和地上部的Cd含量顯著下降;隨K濃度的提高,2個(gè)品種幼苗根系細(xì)胞壁和細(xì)胞液中的Cd含量顯著下降,但細(xì)胞壁中Cd含量的分配比例增大而細(xì)胞液中Cd含量的分配比例則減小。在含2.7μmol·L~(- 1)Cd和30 mmol·L~(- 1)K的培養(yǎng)液中分別添加5 mmol·L~(- 1)TEA或0.2 mmol·L~(- 1)La Cl3后,2個(gè)品種幼苗根系和地上部的Cd和K含量均顯著下降,其中,La Cl3處理組的根系Cd含量降幅高于TEA處理組,但La Cl3處理組的根系K含量降幅則低于TEA組。NSCCs對(duì)品種‘T優(yōu)705’幼苗根系和地上部Cd吸收的貢獻(xiàn)率顯著低于品種‘湘早秈24’幼苗,而K專性通道對(duì)品種‘T優(yōu)705’幼苗根系K吸收和地上部Cd吸收的貢獻(xiàn)率則顯著低于品種‘湘早秈24’幼苗。研究結(jié)果顯示:添加外源K可緩解Cd對(duì)水稻幼苗生長(zhǎng)的抑制作用,并通過提高細(xì)胞壁與Cd的結(jié)合能力來降低細(xì)胞液中Cd的積累,以此減弱幼苗對(duì)Cd的吸收和轉(zhuǎn)運(yùn)能力;幼苗體內(nèi)的K和Cd均可通過K專性通道和NSCCs轉(zhuǎn)運(yùn),其中,K吸收和轉(zhuǎn)運(yùn)主要通過K專性通道完成,而Cd吸收和轉(zhuǎn)運(yùn)主要通過NSCCs完成。此外,品種‘T優(yōu)705’可能具有多種離子通道參與Cd的吸收和轉(zhuǎn)運(yùn),而品種‘湘早秈24’主要依賴NSCCs參與Cd的吸收和轉(zhuǎn)運(yùn),且后者對(duì)K的吸收和積累強(qiáng)于前者。
[Abstract]:In this paper, the relative growth amount of seedlings of rice Oryza sativa Linn.) and low CD accumulation cultivar 'Xiangzaoxian' was studied under different concentrations of Cd(0.0 and 2.7 渭 mol 路L ~ (-1) CD and K030 and 60 mmol 路L ~ (-1) K ~ (-1), respectively, and the low CD accumulation variety 'Xiangzaoxian' ('Xiangzaoxian 24') was used as the experimental material, and the relative growth amount of the two cultivars was studied under different concentrations of Cd(0.0 and Cd(0.0 (2.7 渭 mol 路L ~ (-1) CD) and K030 and 60 mmol 路L ~ (-1) K). The CD content and its subcellular distribution in roots and shoots were compared, and the CD and K contents in roots and shoots were analyzed after adding ion channel active inhibitors TEA and La Cl3. The effects of NSCCs (non-selective cationic channel) and K specific channel on CD and K uptake contribution rates in roots and shoots of two cultivars were compared. The results showed that compared with single CD treatment group (2.7 渭 mol 路L ~ (-1) mol 路L ~ (-1) CD), Cd-K double treatment group was 2.7 渭 mol 路L ~ (-1) ~ (-1) Cd-30 mmol 路L ~ (-1) mmol 路L ~ (-1). The relative growth of seedlings of two cultivars, 1K and 2.7 渭 mol 路L ~ (-1) Cd-60 mmol 路L ~ (-1), increased significantly. However, CD content in root and shoot decreased significantly, and CD content in root cell wall and cell fluid decreased significantly with the increase of K concentration. However, the distribution ratio of CD content in cell wall increased and that in cell fluid decreased. After adding 5 mmol 路L ~ (-1) tea or 0.2 mmol 路L ~ (-1) La Cl3 to the culture medium containing 2.7 渭 mol 路L ~ (-1) ~ (-1) and 30 mmol 路L ~ (-1) K, respectively, the roots and shoot of the two cultivars were obtained. The contents of CD and K decreased significantly. The decrease of CD content in roots of La Cl3 treatment group was higher than that of TEA treatment group, but that of La Cl3 treatment group was lower than that of TEA group. NSCCs contribution rate to root and shoot CD uptake of the seedling was significantly lower than that of 'Xiangzao indica rice 24' seedling. The contribution of K specific pathway to root K uptake and shoot CD uptake was significantly lower than that of 'Xiangzao Indica 24' seedling. The results showed that exogenous K could alleviate the inhibition of CD on rice seedling growth. CD accumulation in the cell fluid was reduced by increasing the binding ability of the cell wall to CD, thus the CD uptake and transport ability of the seedlings were weakened, and both K and CD were transported through K specific channels and NSCCs. The absorption and transport of K and CD are mainly through K specific channels, and CD uptake and transport are mainly through NSCCs. In addition, the variety TY705'may have a variety of ion channels involved in CD absorption and transport. The variety 'Xiangzao indica' mainly depends on NSCCs to participate in CD absorption and transport, and the latter's absorption and accumulation of K is stronger than that of the former.
【作者單位】： 農(nóng)業(yè)部環(huán)境保護(hù)科研監(jiān)測(cè)所生態(tài)毒理與環(huán)境修復(fù)研究中心;天津師范大學(xué)生命科學(xué)學(xué)院天津市動(dòng)植物抗性重點(diǎn)實(shí)驗(yàn)室;河南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：2015年中國(guó)農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目(2015-cxgc-lzq) 國(guó)家農(nóng)業(yè)部公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201403015)
【分類號(hào)】：S511.21;X503.231

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