本文關鍵詞： 鄰苯二甲酸二丁酯 吸附 微生物 礦物 DMPP　出處：《湖南農(nóng)業(yè)大學》2015年碩士論文　論文類型：學位論文

【摘要】：鄰苯二甲酸二丁酯(DBP)作為鄰苯二甲酸酯類的一種,是環(huán)境中較為常見的環(huán)境激素類有機污染物。本文從DBP染毒后的土壤污染樣品中篩選出一株對DBP有較高耐受性的微生物伯克霍爾德菌(Burkholderia),研究其對土壤中DBP的吸附行為和機制,及其與三種礦物(針鐵礦、高嶺土、蒙脫石)組成的復合體系對DBP的吸附行為和機制。在研究Burkholderia對DBP的吸附行為時,從吸附動力學、吸附等溫模型擬合、解吸等方面進行分析,并考察了pH值、不同濃度的3,4-二甲基吡唑磷酸鹽(DMPP)等因素對菌體吸附DBP的影響,結(jié)合傅立葉轉(zhuǎn)換紅外光譜(FTIR)對Burkholderia吸附DBP前后的表面基團的變化進行了分析；在研究細菌-礦物復合體系吸附DBP時,考察了復合體系的吸附量(率)和解吸量(率),及細菌礦物之間不同比例、不同DMPP濃度的影響。結(jié)合掃描電子顯微鏡(Scanning Electron Microscope, SEM)觀察Burkholderia與三種礦物復合后的表面形貌；同時通過三種復合體系疏水性實測值與預估值之間的對比,進一步闡明了復合體系的吸附機理。通過以上大量實驗初步探明了DBP在Burkholderia和三種礦物界面的吸附行為及機制。本文的研究結(jié)論有如下幾點：(1) Burkholderia吸附DBP的過程符合Freundlich吸附等溫模型,相關性R2=0.94,吸附等溫式為：qe=2A6×Ce1/1.02。(2)解吸實驗發(fā)現(xiàn),Burkholderia對DBP的吸附有物理吸附和化學吸附,其中主要是化學吸附。(3)紅外光譜分析結(jié)果表明,細菌對DBP的吸附主要是通過C-H的伸縮振動而相互作用在一起的。同時,細菌表面的活性基團和細胞壁也參與了吸附行為。(4)在1：1的混合比例下,復合體對DBP吸附的實測值與理論值之間的差異最大,對DBP吸附的影響較大。細菌-針鐵礦和細菌-蒙脫石的實際吸附量比理論值分別提高了1.10 mg/g和1.83 mg/g,而細菌-高嶺土則下降了1.16 mg/g。(5)通過掃描電鏡觀察發(fā)現(xiàn),微生物與三種礦物結(jié)合形態(tài)不同,與針鐵礦和蒙脫石結(jié)合緊密,與高嶺土結(jié)合松散。(6)不同濃度的DMPP加入對細菌的生長、DBP的吸附和復合體系吸附DBP均有影響,且2 mmol/L的DMPP可以較大提高細菌-蒙脫石復合體系對DBP的吸附。
[Abstract]:Dibutyl phthalate (DBP) is a kind of phthalate ester. It is a common environmental hormone organic pollutant in the environment. In this paper, a microorganism with high tolerance to DBP was isolated from soil contaminated samples exposed to DBP. Burkholderia. The adsorption behavior and mechanism of DBP in soil and its relationship with three minerals (goethite and kaolin) were studied. The adsorption behavior and mechanism of DBP in montmorillonite (MMT) composite system. The adsorption kinetics and isotherm model of DBP adsorption by Burkholderia were fitted. The effects of pH value and different concentration of 3o 4- dimethylpyrazole phosphate on the adsorption of DBP were investigated. The changes of surface groups before and after Burkholderia adsorption on DBP were analyzed by Fourier transform infrared spectroscopy (FTIR). In the study of the adsorption of DBP by bacteria-mineral complex system, the adsorption capacity (rate) and desorption capacity (ratio) of the composite system were investigated, as well as the different ratios between bacteria and minerals. Combined with scanning electron microscope (SEM), scanning Electron Microscope. The surface morphology of Burkholderia combined with three minerals was observed. At the same time, the hydrophobicity of the three composite systems was compared with the estimated values. The adsorption mechanism of the composite system was further elucidated. The adsorption behavior and mechanism of DBP at the interface of Burkholderia and the three minerals were preliminarily proved by a large number of experiments mentioned above. The conclusion of this paper is as follows:. The following points:. (. 1) the process of DBP adsorption by Burkholderia accords with the isotherm model of Freundlich adsorption. The desorption experiment showed that the adsorption isotherm equation was: 1: QE 2A6 脳 ce 1 / 1.02.2.2. The adsorption of DBP by Burkholderia includes physical adsorption and chemisorption, mainly chemisorption. The adsorption of DBP by bacteria is mainly through C-H stretching vibration and interaction. At the same time. The active group and cell wall on the surface of bacteria also participated in the adsorption behavior. 4) under the mixed ratio of 1: 1, the difference between the measured values and the theoretical values of DBP adsorption by the complex was the largest. The adsorption capacity of bacteria-goethite and bacteria-montmorillonite was 1.10 mg/g and 1.83 mg/g higher than the theoretical values, respectively. On the other hand, bacteria-kaolin decreased by 1.16 mg / g 路L ~ (5). By scanning electron microscope, it was found that the microorganism binds closely with goethite and montmorillonite in different forms with the three minerals. The addition of different concentrations of DMPP to kaolinite could affect the adsorption of DBP on the growth of bacteria and the adsorption of DBP in the composite system. The adsorption of DBP on the bacteria-montmorillonite composite system could be greatly improved by 2 mmol/L DMPP.
【學位授予單位】：湖南農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：X592;X172

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